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Isoquinoline alkaloid isolated from red clover, preparation method and use

A technology of isoquinoline alkaloids and red clover, applied in the field of medicine, can solve the problems of large side effects of aspirin, ineffective anti-platelet treatment effect, etc., and achieve the effects of less toxic and side effects, significant anti-platelet aggregation, and novel structure

Active Publication Date: 2019-07-30
NANYANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditionally, aspirin is used for antiplatelet therapy, but aspirin has relatively large side effects and the effect of antiplatelet therapy is not efficient

Method used

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  • Isoquinoline alkaloid isolated from red clover, preparation method and use
  • Isoquinoline alkaloid isolated from red clover, preparation method and use
  • Isoquinoline alkaloid isolated from red clover, preparation method and use

Examples

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Effect test

Embodiment 1

[0033] The present invention provides a kind of preparation method of the new isoquinoline alkaloid that is separated from red clover, comprises the steps:

[0034] S1. Grind the dried red clover, soak it in 95% ethanol for 20 hours, heat and reflux for extraction 3 times, 3 hours each time, dry the filtrate at low temperature until it has no alcohol smell, and obtain the thick paste of red clover ;

[0035] S2, uniformly disperse the thick red clover paste obtained in step S1 in a 1.0% HCl solution, sonicate for 50 minutes, let stand for 18 hours, and filter to obtain the supernatant;

[0036] S3. Adjust the supernatant liquid obtained in step S2 to pH=12 with aqueous ammonia, and extract with organic solvent in turn. The organic solvent is chloroform or acetone, and the extracts are concentrated and dried to obtain chloroform thick paste or acetone thick paste. Born turbidity is used. Determination of the platelet aggregation rate of chloroform thick paste and acetone thick...

Embodiment 2

[0050] The invention provides a preparation method of a novel isoquinoline alkaloid isolated from red clover, comprising the steps of:

[0051] S1. Grind the dried red clover, soak it in 95% ethanol for 20 hours, heat and reflux for extraction 3 times, 3 hours each time, dry the filtrate at low temperature until it has no alcohol smell, and obtain the thick paste of red clover ;

[0052] S2, uniformly disperse the thick red clover paste obtained in step S1 in a 1.0% HCl solution, sonicate for 50 minutes, let stand for 18 hours, and filter to obtain the supernatant;

[0053] S3. Adjust the supernatant obtained in step S2 to pH = 12 with aqueous ammonia, extract with an organic solvent in sequence, and concentrate and dry each extract to obtain a thick paste;

[0054] S4. Dissolve the active part, that is, the thick paste in step S3 with petroleum ether, put it on the silica gel column by wet method, and perform gradient elution with petroleum ether-ethyl acetate elution system...

Embodiment 3

[0067] This embodiment provides a method for preparing a new isoquinoline alkaloid isolated from red clover, comprising the following steps:

[0068] S1. Grind the dried red clover, soak it in 95% ethanol for 20 hours, heat and reflux for extraction 3 times, each time for 3 hours, and dry the filtrate in a low temperature environment until it has no alcohol smell, and the red clover thick paste is obtained ;

[0069] S2, uniformly disperse the thick red clover paste obtained in step S1 in a 1.0% HCl solution, sonicate for 50 minutes, let stand for 18 hours, and filter to obtain the supernatant;

[0070] S3. Adjust the supernatant obtained in step S2 to pH = 12 with aqueous ammonia, extract with an organic solvent in sequence, and concentrate and dry each extract to obtain a thick paste;

[0071] S4. Dissolving the active part, that is, the thick paste in step S3, with petroleum ether, put it on a silica gel column by a wet method, and use a petroleum ether-ethyl acetate eluti...

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Abstract

The invention provides neoisoquinoline alkaloid separated from red clover as well as a preparation method and application thereof. The preparation method comprises the following steps: grinding driedred clover, soaking in ethanol, heating and performing reflux extraction, and drying the filtrate in a low-temperature environment until no alcohol taste exists so as to obtain total thick paste of the red clover; uniformly dispersing the total thick paste of the red clover into a HCl solution, performing ultrasonic treatment, standing and filtering to obtain the supernatant; extracting the supernatant with an organic solvent to obtain thick paste; eluting with a petroleum ether-ethyl acetate elution system, a cyclohexane-ethyl acetate elution system and methanol in sequence, and finally repeatedly eluting with acetonitrile, thereby obtaining the compound. The compound is novel in structure and simple and convenient in preparation method, has an obvious anti-platelet aggregation effect, and has high medicinal value.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a new isoquinoline alkaloid isolated from red clover, a preparation method and application. Background technique [0002] Red clover (Trifolium pratense L.) is a perennial forage grass belonging to the family Leguminosae, also known as red clover, red Dutch clover, etc. Native to Asia Minor and Southeast Europe, widely distributed in temperate and subtropical regions. There are a large number of distributions in Xinjiang, Qinghai, Jilin and Yunnan-Guizhou Plateau in my country. In recent years, more and more attention has been paid to the research on red clover. Studies have found that red clover contains isoflavones, volatile oils, selenium-containing compounds, crude protein, calcium, potassium, magnesium, phosphorus, vitamins, niacin and other nutrients. Modern medical research has found that red clover has various pharmacological effects such as regulating hormone balance,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D491/056A61P7/02
CPCA61P7/02C07D491/056
Inventor 马勤阁魏荣锐桑志培柳文敏
Owner NANYANG NORMAL UNIV
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