Method for extracting and purifying anti-hypoxic polysaccharides from Qaidam agaricus bitorquis

Abstract

The invention discloses a method for extracting and purifying anti-hypoxic polysaccharides from Qaidam agaricus bitorquis. The method mainly comprises the following steps: extracting crude polysaccharides by an ultrasonic method, discoloring the crude polysaccharides with activated carbon, purifying and eluting various single components by DEAE-52 cellulose column chromatography, removing small molecule substances by utilizing a dialysis membrane, and finally performing rotary evaporation and concentration on the various single components, thereby obtaining the purified single components of polysaccharides. The method has the advantages of high efficiency and high yield, pigment macromolecular substances, proteins and other impurities can be effectively removed, adsorbable ionic substancesare separated by the DEAE cellulose column chromatography, and neutral polysaccharides can smoothly flow out, so that the crude products are removed to obtain refined products, and the separation aimis achieved. The yield of fruiting body crude polysaccharides subjected to ultrasonic extraction reaches 128.25 mg / g; the discoloring ratio of the discolored crude polysaccharides by activated carbonis 76.5%; 3-4 single components of polysaccharides can be separated by the DEAE-52 cellulose column chromatography. The purification degree of the pure polysaccharides of Qaidam agaricus bitorquis can be improved and increased, and the finished polysaccharides derived from the Qaidam agaricus bitorquis at high purity can be obtained.

Description

technical field [0001] The invention relates to the technical field of biochemistry, in particular to a method for extracting and purifying polysaccharides from Qaidam phagaphyta mushrooms. Background technique [0002] Qaidam big fat mushroom is a wild large edible fungus with very important classification under the special ecological environment conditions of the Qaidam Basin in the Qinghai-Tibet Plateau. Because it grows in a harsh environment, it has the characteristics of huge fruiting bodies, strong stress resistance, firm underground, and low-temperature fruiting. It is a special strain of big fat mushrooms. The fruit body of Qaidam big fat mushroom contains 24.0% crude protein, 2.10% crude fat, 17.32% crude fiber, 34.91% carbohydrate and 13.65% ash. According to the solubility of edible fungus polysaccharides, polar macromolecular compounds are easily soluble in water but insoluble in ethanol. At present, the commonly used extraction methods are: hot water extracti...

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Problems solved by technology

However, because Qaidam big fat mushroom is a special fungus that grows in the Qaidam Basin of the Qinghai-Tibet Plateau, there is almost no research on it abroad, and although there are many research reports on it in China, it has not been publicly adopted. The technical scheme for extracting and purifying the Qaidam big fat mushroom polysaccharide by the above method

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