Preparation method of zinc-rich lactobacillus plantarum additive
A technology of Lactobacillus plantarum and additives, applied in the field of feed additives, can solve the problems of imperfect manufacturing process, difficulty in large-scale promotion and use, and high production cost
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Embodiment 1
[0034] A preferred embodiment of the present invention provides a kind of preparation method of high-zinc-resistant Lactobacillus plantarum strain, comprising the following steps:
[0035] 1) Lactobacillus plantarum is inoculated on the improved MRS solid medium containing different zinc concentrations by the method of coating the plate, to determine the tolerance of its zinc ions;
[0036] 2) With the determined zinc ion tolerance concentration as the initial concentration, inoculate Lactobacillus plantarum on the improved MRS liquid medium containing zinc ions for cultivation, and then use the inoculation loop to pick the bacteria liquid in the improved MRS containing the same zinc ion concentration. Streak culture is carried out on the solid plate medium, which is the first generation; pick the larger colony on the solid plate and insert it into the improved MRS liquid medium containing zinc ions for culture, and then use the inoculation loop to pick the bacterial liquid in ...
Embodiment 2
[0040] The present embodiment provides a kind of preparation method of zinc-rich Lactobacillus plantarum additive, comprising the following steps:
[0041] 1) Strain culture on slant: Streak inoculation of high zinc-resistant and zinc-rich Lactobacillus plantarum on the slant of the improved MRS solid medium with a sugar content of 2-5%, a zinc content of 20-500ug / ml, and a pH value of 5.5-7.0 On, culture temperature 35-40 ℃, anaerobic culture 36-48h;
[0042] 2) First-level liquid seed culture: add 5ml of sterilized physiological saline to the Lactobacillus plantarum test tube on the slope, wash and mix the colonies on the slope, draw 2.5ml of the bacteria solution and inoculate it with sugar content 2-5%, zinc content 20 -500ug / ml, pH value 5.5-7.0 250mL improved MRS liquid medium Erlenmeyer flask, culture temperature 35-40°C, anaerobic culture 15-24h, to obtain a first-class liquid seed culture medium;
[0043] 3) Secondary liquid seed culture: Inoculate 200ml of primary liq...
Embodiment 3
[0047] This example provides a detection method and result of the effect of zinc-rich Lactobacillus plantarum additive on the growth performance of growing and finishing pigs.
[0048] 1. Experimental animals
[0049] Experimental animals: Select 120 “Du × Chang × Da” three-way hybrid pigs with a body weight of about 18 kg, and randomly divide them into 3 groups according to the principles of the same genetic background, similar body weight, and the same litter, with 4 replicates in each group, and 10 replicates in each replicate. head.
[0050] 2. Test materials
[0051] 1) Zinc-rich Lactobacillus plantarum powder products
[0052] The method of Example 2 was used to prepare the zinc-rich Lactobacillus plantarum bacterial powder product, and the zinc content was 3000 mg / kg.
[0053] 2) Test diet
[0054] The diet formula includes: corn 55.24%, barley 7%, expanded soybean 6.23%, dehulled soybean meal 20.68%, subflour 6%, cotton meal 4%, soybean oil 0.5%, salt 0.35%, and vi...
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