Culture medium and method for carrying out lotus embryo rescue and offspring fast growth and development

A growth and rooting medium technology, applied in botany equipment and methods, horticultural methods, plant regeneration, etc., can solve the problem of sexual reproduction of immature lotus seeds, save manpower, material resources and time costs, simple operation, The effect of high reproduction coefficient

Active Publication Date: 2018-11-23
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem that immature lotus seeds cannot be sexually reproduced by conventional methods, the present invention provides a culture medium and

Method used

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  • Culture medium and method for carrying out lotus embryo rescue and offspring fast growth and development
  • Culture medium and method for carrying out lotus embryo rescue and offspring fast growth and development
  • Culture medium and method for carrying out lotus embryo rescue and offspring fast growth and development

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Preparation of embryo rescue medium: add 2.0mg / L 2,4-D, 0.5mg / L 6-BA, 1.0mg / L KT, 80mg / L VC, 30g / L to MS liquid medium sucrose, 1.16g / L plant gel, adjust the pH to be 5.8, and sterilize for subsequent use.

[0020] Preparation of rooting medium: Add 2.0mg / L NAA, 0.5mg / L 6-BA, 1.0mg / L KT, 30g / L sucrose, 1.16g / L plant gel to MS liquid medium , adjust the pH to 5.8, and sterilize for later use.

[0021] Adjust the content of 2,4-D, 6-BA, KT, VC or NAA in the above medium to prepare corresponding control medium. For the convenience of comparison, each medium is expressed as follows:

[0022] Embryo rescue medium of the present invention: MS+2,4-D 2.0mg / L+6-BA 0.5mg / L+KT 1.0mg / L+VC 80mg / L,

[0023] Embryo rescue medium control 1: MS+2,4-D 1.0mg / L+6-BA 0.5mg / L+KT 1.0mg / L+VC 80mg / L,

[0024] Embryo rescue medium control 2: MS+2,4-D 2.0mg / L+6-BA 0.2mg / L+KT 1.0mg / L+VC 80mg / L,

[0025] Embryo rescue medium control 3: MS+2,4-D 2.0mg / L+6-BA 0.5mg / L+KT 0.5mg / L+VC 80mg / L,

[002...

Embodiment 2

[0039] (1) The pretreatment of lotus embryos to save offspring seeds is as follows: soak immature young lotus seeds in detergent water for 10 minutes, rinse with running water, disinfect with 75% alcohol for 5 minutes, soak and disinfect with 0.05% mercury liter for 8 minutes , washed 5 times with sterile water, and the young lotus embryos in the endosperm were taken as explants for later use.

[0040] (2) The explants obtained after the pretreatment were inoculated in the embryo rescue medium for induction culture, and the culture conditions were: temperature 28° C., light intensity 1500 lux, light 12 h / d.

[0041] (3) Two weeks later, after the immature embryos survived and developed clustered buds, they were transferred to the rooting medium for induction culture. The culture conditions were: temperature 28°C, light intensity 1800lux, light 14h / d, and grow out after one week. The root system becomes a complete test-tube plantlet.

[0042] See Table 1 for the growth of clus...

Embodiment 3

[0050](1) Pretreatment of lotus embryos to save offspring seeds is as follows: soak lotus pods fertilized for 8-10 days with detergent water for 3 minutes, rinse with running water, sterilize with 75% alcohol for 5 minutes, soak and sterilize with 0.05% mercury liter for 8 minutes, no Bacteria were washed 5 times with water, and the ovaries in the lotus pods were taken as explants for subsequent use.

[0051] (2) The explants obtained after the pretreatment were inoculated in the embryo rescue medium for induction culture, and the culture conditions were: temperature 28° C., light intensity 1500 lux, and light 12 h / d.

[0052] (3) Two weeks later, after the immature embryos survived and developed clustered buds, they were transferred to the rooting medium for induction culture. The culture conditions were: temperature 28°C, light intensity 1800lux, light 12h / d, grow out after one week The root system becomes a complete test-tube plantlet.

[0053] See Table 3 for the clustere...

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Abstract

The invention discloses a culture medium and a method for carrying out lotus embryo rescue and offspring fast growth and development, and belongs to the technical field of plant tissue culture and fast propagation technologies. The culture medium comprises an embryo rescue culture medium and a rooting culture medium, wherein the embryo rescue culture medium is prepared from 2.0mg/L of MS (Mass Spectrum)+2,4-D (2,4-Diclorophenoxyacetic acid), 0.5m/L of 6-BA (6-Benzyladenine) and 80m/L of VC (Ascorbic Acid); the rooting culture medium is prepared from 2.0m/L of MS+NAA (Naphthylacetic Acid), 0.5mg/L of 6-BA and 1.0m/L of KT (Kinetin). The method comprises the following steps: pretreating lotus seeds requiring for the embryo rescue, sequentially inoculating the pretreated lotus seeds to the embryo rescue culture medium and the rooting culture medium for carrying out induction culture, enabling the lotus seeds to grow, and rooting. According to the culture medium and the method, disclosed by the invention, the problem that immature lotus seeds cannot grow and propagate through a conventional method is solved, and healthy and fast propagation and growth of tissue culture seedlings can berealized in a short period; the culture medium is convenient to operate, is easy to popularize and can realize large-scale production.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture and rapid propagation, in particular to a culture medium and method for saving lotus embryos and rapid growth and development of offspring. Background technique [0002] Lotus has both edible and ornamental functions, and has great economic value. Lotus can reproduce both asexually and sexually. During sexual reproduction, lotus seeds need to be completely mature naturally, and the skin is brown. After natural dehydration, the water content drops significantly, and the volume becomes smaller than before. If the lotus seeds are immature, the embryo cannot germinate and grow naturally. A large number of Hualien varieties cannot obtain mature lotus seeds under natural conditions. In addition, when carrying out cross-breeding of lotus, it is difficult to obtain mature lotus seeds in some cross-breeding combinations. Therefore, there is an urgent need for an effective technical means t...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 胡中立李晔刁英郑兴汶汪超周明全
Owner WUHAN UNIV
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