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A detection kit for α2-macroglobulin (α2-mg)

A technology for detecting kits and macroglobulins, applied in biological testing, material inspection products, etc., can solve problems such as poor stability and low sensitivity, and achieve the effects of improving stability, strong anti-interference, and maintaining antibody stability

Active Publication Date: 2020-04-28
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the above problems, the object of the present invention is to provide a detection kit for α2-macroglobulin (α2-MG), which adopts latex-enhanced immune turbidimetric method, which overcomes the disadvantages of low sensitivity and poor stability of common immune turbidimetric method , can effectively detect the content of α2-macroglobulin (α2-MG), and has the advantages of high sensitivity and good stability; another purpose of the present invention is to provide the preparation and use method of the detection kit

Method used

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  • A detection kit for α2-macroglobulin (α2-mg)
  • A detection kit for α2-macroglobulin (α2-mg)
  • A detection kit for α2-macroglobulin (α2-mg)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The detection reagents for α2-macroglobulin (α2-MG) provided in this example include reagent R1 and reagent R2:

[0042] The components of reagent R1 include:

[0043] ACES (N-carbamoylmethylethanesulfonic acid) buffer (pH=7.2, 25°C) 65mmol / L,

[0044] NaCl 9g / L,

[0045] PEG-6000 15g / L,

[0046] Sodium undecylcarboxymethyl imidazoline acetate 0.5g / L,

[0047] Preservative sodium pyrithione 0.5g / L;

[0048] The components of reagent R2 include:

[0049] ACES (N-carbamoylmethylethanesulfonic acid) buffer (pH=7.3, 25°C) 70mmol / L,

[0050] Goat anti-human α2-macroglobulin (α2-MG) antibody coated latex particles 0.25% (W / V),

[0051] Casein 0.5g / L,

[0052] Gelatin 0.1g / L,

[0053] Glycerin 10g / L,

[0054] Trehalose 15g / L,

[0055] Sodium undecylcarboxymethyl imidazoline acetate 0.3g / L,

[0056] Preservative sodium pyrithione 0.5g / L.

[0057] The usage method of this embodiment reagent:

[0058] The α2-macroglobulin (α2-MG) detection kit described in this examp...

Embodiment 2

[0060] The α2-macroglobulin (α2-MG) detection reagent described in this example includes reagent R1 and reagent R2 (the detection method is the same as that in Example 1):

[0061] The components of reagent R1 include:

[0062] ACES (N-carbamoylmethylethanesulfonic acid) buffer (pH=7.2, 25°C) 65mmol / L,

[0063] NaCl 9g / L

[0064] PEG-6000 18g / L,

[0065] Sodium undecylcarboxymethyl imidazoline acetate 0.5g / L,

[0066] Preservative sodium pyrithione 0.5g / L;

[0067] The components of reagent R2 include:

[0068] ACES (N-carbamoylmethylethanesulfonic acid) buffer (pH=7.3, 25°C) 70mmol / L,

[0069] Goat anti-human α2-macroglobulin (α2-MG) antibody coated latex particles 0.30% (W / V),

[0070] Casein 0.5g / L,

[0071] Gelatin 0.1g / L,

[0072] Glycerin 15g / L,

[0073] Trehalose 20g / L,

[0074] Sodium undecylcarboxymethyl imidazoline acetate 0.3g / L,

[0075] Preservative sodium pyrithione 0.5g / L.

Embodiment 3

[0077] The detection reagent for α2 macroglobulin (α2-MG) described in this example includes reagent R1 and reagent R2 (the detection method is the same as that in Example 1):

[0078] The components of reagent R1 include:

[0079] ACES (N-carbamoylmethylethanesulfonic acid) buffer (pH=7.2, 25°C) 65mmol / L,

[0080] NaCl 9g / L

[0081] PEG-6000 20g / L,

[0082] Sodium undecylcarboxymethyl imidazoline acetate 0.5g / L,

[0083] Preservative sodium pyrithione 0.5g / L;

[0084] The components of reagent R2 include:

[0085] ACES (N-carbamoylmethylethanesulfonic acid) buffer (pH=7.3, 25°C) 70mmol / L,

[0086] Goat anti-human α2-macroglobulin (α2-MG) antibody coated latex particles 0.40% (W / V)

[0087] Casein 0.5g / L

[0088] Gelatin 0.1g / L,

[0089] Glycerin 20g / L,

[0090] Trehalose 25g / L,

[0091] Sodium undecylcarboxymethyl imidazoline acetate 0.3g / L,

[0092] Preservative sodium pyrithione 0.5g / L.

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Abstract

The invention relates to the technical field of in vitro immunodiagnosis detection of alpha 2-macroglobulin (alpha 2-MG), in particular to an alpha 2-MG detection kit. A reagent R1 contains a buffer solution, NaCl, PEG-6000, undecylcarboxymethyl sodium type imidazoline acetate, and a preservative, a reagent R2 contains a buffer solution, goat anti-human alpha 2-MG antibody-coated latex particles,casein, gelatin, glycerin, trehalose, undecylcarboxymethyl sodium type imidazoline acetate, preservative. The kit disclosed by the invention significantly improves the stability and linear range of the reagent, and significantly enhances the sensitivity and accuracy of the reagent.

Description

technical field [0001] The invention belongs to the technical field of in vitro immunodiagnosis and detection, and in particular relates to a kit for detecting α2-macroglobulin (α2-MG) by latex-enhanced immune turbidimetry and methods for its preparation and use. Background technique [0002] α2-macroglobulin is the protein with the largest molecular weight in plasma. Synthesized in hepatocytes and mononuclear phagocytes, with a half-life of about 5 days, but when combined with proteolytic enzymes to form a complex, its clearance rate is accelerated. The characteristic of α2-MG is that it can combine with various molecules and ions, especially it can combine with many proteolytic enzymes to affect the activity of these enzymes, and play a role in selectively maintaining the activity of certain proteases. may be of significance. In addition, it is closely related to the development and function of lymphoreticular system cells. [0003] Determination of α2-macroglobulin con...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 李志明胡晓飞赵民王美丽
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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