Unlock instant, AI-driven research and patent intelligence for your innovation.

Efficient enrichment device for pathogenic microorganisms in water environment and enrichment device thereof

A technology for pathogenic microorganisms and water environment, which is applied in the methods of supporting/immobilizing microorganisms, biochemical equipment and methods, and methods of sampling biological materials, etc., which can solve the problems affecting the repeated reuse of adsorbent materials and the limited ability to reuse filter membranes. , Cumbersome regeneration of adsorbent materials, etc., to achieve the effect of wide use, strong wear resistance and high precision

Pending Publication Date: 2018-11-30
INST OF ENVIRONMENTAL MEDICINE & OCCUPATIONAL MEDICINE ACAD OF MILITARY MEDICINE ACAD OF MILITARY SCI
View PDF13 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Filtration is currently the most commonly used method for enriching pathogenic microorganisms. Generally, microfiltration membranes, ultrafiltration membranes, etc. are the main methods. However, due to the limited ability of the filtration membrane to be reused, its cost is relatively high.
The flocculation method requires the use of a large amount of flocculant to achieve the purpose of enrichment, and the use of a large amount of flocculant will cause certain potential harm to the environment
In the adsorption method, the regeneration of the adsorbent material is cumbersome, which affects the repeated use of the adsorbent material.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Efficient enrichment device for pathogenic microorganisms in water environment and enrichment device thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Enrichment of Cryptosporidium oocysts and Giardia cysts in 100L tap water

[0053] Take 100L of urban tap water, and after autoclaving at 121°C, add Cryptosporidium oocysts and Giardia cysts to artificially pollute the tap water so that the concentrations reach 1 / L respectively. Select a filter element with a pore size of 1.0 μm, start the peristaltic pump 3 for water sample filtration, control the flow rate of the water sample at 2L / min, make the water sample enter the filter element sleeve 2 from the water sample inlet 4, and filter through the filter element 1, after the water sample is filtered Stop filtration with peristaltic pump 3.

[0054] Afterwards, put the eluent into the eluent tank 9, start the peristaltic pump 7 for elution, make the eluent pass through the middle gap of the filter element and elute the enriched microorganisms by backflushing, and wait until the eluent completely enters After the gap between filter element 1 and filter element sleeve 2, r...

Embodiment 2

[0056] Enrichment of Salmonella and Shigella in 100L Tap Water

[0057] Take 100L of urban tap water, after autoclaving at 121°C, add Salmonella and Shigella bacterial liquids, and artificially pollute the tap water so that the concentrations reach 10CFU / L respectively. Select a filter element with a pore size of 0.45 μm, start the peristaltic pump 3 for water sample filtration, control the flow rate of the water sample at 2L / min, make the water sample enter the filter element sleeve 2 from the water sample inlet 4, and filter through the filter element 1, after the water sample is filtered Stop filtration with peristaltic pump 3.

[0058] Afterwards, put a certain amount of eluent into the eluent tank 9, start the peristaltic pump 7 for eluting, make the eluent pass through the middle space of the filter element 1 and carry out the elution of the enriched microorganisms by backflushing, and wait for the elution After all the liquid enters the gap between the filter element 1...

Embodiment 3

[0060] Enrichment of Leptospira in 100L Tap Water

[0061] Take 100L of urban tap water, after autoclaving at 121°C, add Leptospira worms, artificially pollute the water sample, and make the concentration reach 1 / L. Select a filter element with a pore size of 1.0 μm, start the peristaltic pump 3 for water sample filtration, control the flow rate of the water sample at 2L / min, make the water sample enter the filter element sleeve 2 from the water sample inlet 4, and filter through the filter element 1, after the water sample is filtered Stop filtration with peristaltic pump 3.

[0062] Afterwards, put a certain amount of eluent into the eluent tank 9, start the peristaltic pump 7 for eluting, make the eluent pass through the middle space of the filter element and carry out the elution of the enriched microorganisms by backflushing. After all of them enter the gap between the filter element 1 and the filter element sleeve 2, remove the filter element sleeve 2 and the filter ele...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
recovery rateaaaaaaaaaa
recovery rateaaaaaaaaaa
Login to View More

Abstract

The invention relates to an efficient enrichment device for pathogenic microorganisms in a water environment, and belongs to the technical field of water environment microbiological detection. The enrichment device comprises a filtering part and a control part, a filter element in the filtering part is placed in an inner cavity of a filter element sleeve and space is formed between the filter element and the inner cavity wall of the filter element sleeve. A water sample water inlet is formed in the top end of the filter element sleeve. A water sample water outlet is formed in the bottom end ofthe filter element. The water sample water outlet passes through the inner wall of the filter element sleeve and extends to the outer side. The control part includes a tee, a water sample filtrationcontrol part and an elution control part. The water sample filtration control part communicates with a first check valve and a peristaltic pump for water sample filtration in sequence according to thewater sample outflow sequence. The elution control part sequentially communicates with an eluant tank, a peristaltic pump for elution and a second check valve according to the flow direction of elution fluid. The water sample water outlet, the first check valve and the second check valve communicate with each other through the tee. The efficient enrichment of the pathogenic microorganisms with alow concentration in the water environment is realized, and technical support is provided for the detection and monitoring of waterborne diseases.

Description

technical field [0001] The invention belongs to the technical field of pathogenic microorganism detection in water environment, and in particular relates to a high-efficiency enrichment device and an enrichment method for pathogenic microorganism in water environment. Background technique [0002] Various pathogenic microorganisms (including bacteria, protozoa, viruses, etc.) existing in the water environment are the main cause of the outbreak of water-borne diseases, which not only endanger the health of the population, but also cause serious economic losses, and even cause serious adverse social conditions. influences. The water-borne transmission of pathogenic microorganisms often leads to mass outbreaks and presents a global distribution, among which pathogenic bacteria, parasites, viruses, etc. are the main incentives. According to statistics, there are more than 1.3 million deaths from diarrhea in children under 5 years of age worldwide due to pathogenic microorganism...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/12
CPCC12M33/14
Inventor 尹静王华然杨忠委李君文金敏杨栋谌志强
Owner INST OF ENVIRONMENTAL MEDICINE & OCCUPATIONAL MEDICINE ACAD OF MILITARY MEDICINE ACAD OF MILITARY SCI