Microbial agent of Bacillus velezensis

A technology of microbial inoculum and bacillus, applied in the directions of microorganisms, microorganisms, biocides, etc., to achieve the effects of large spore production, diverse formulations and convenient use methods

Active Publication Date: 2018-12-07
NORTH CHINA PHARMA GROUP AINO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bacillus Velez is a new species of Bacillus named by Ruiz-Garcfa in 2005. It is a new type of biocontrol bacteria. In recent years, relevant research has gradually increased. Some researchers have found that Bacillu...

Method used

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  • Microbial agent of Bacillus velezensis
  • Microbial agent of Bacillus velezensis
  • Microbial agent of Bacillus velezensis

Examples

Experimental program
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Effect test

preparation example Construction

[0043] Two, the preparation of the former powder of Bacillus Velez 1721

[0044] Using Bacillus Velez 1721 as an active ingredient can be used to make a variety of microbial preparations. To prepare the bacteria for the preparation of microbial inoculum, it is necessary to prepare the original powder of Bacillus Velez 1721. The preparation steps of the original powder are as follows:

[0045] A. Inoculate Bacillus Velez 1721 into LB liquid medium, shake culture to bacterial density OD 600 The value is 0.6-0.8, and the strain is obtained;

[0046] The content of tryptone in the LB liquid medium is 9-11g / L, the content of yeast extract is 4-6g / L, the content of sodium chloride is 9-11g / L, and the pH value of the LB medium is 7.0-7.2;

[0047] B. Insert the bacterial classification in step A into the seed tank by 9~11% of the medium volume used in the seed tank, the cultivation temperature of the seed tank is 35~37°C, the tank pressure is 0.05~0.07MPa, and the ventilation ratio ...

Embodiment 1

[0073] The preparation steps of Bacillus 1721 granules are as follows:

[0074] Prepare granules according to the above steps, and the specific composition content and process parameters are shown in the following table:

[0075] Table 2

[0076] project

Embodiment 2

[0078] The preparation steps of Bacillus Velez 1721 wettable powder are as follows:

[0079] Prepare the wettable powder according to the above steps, and the specific component content and process parameters are shown in the following table:

[0080] table 3

[0081]

[0082]

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Abstract

The invention discloses a microbial agent of Bacillus velezensis, belonging to the field of development and application of microorganisms. The Bacillus velezensis is Bacillus velezensis 1721, which has been preserved in China General Microbiological Culture Collection Cente on January 29, 2018, with an accession number of CGMCC No. 15324. The Bacillus velezensis 1721 provided by the invention maybe made into a wettable powder, a suspending agent, a water dispersible granule or a granule, and has high antibacterial activity in each dosage form; when acting on peanuts, the microbial agent of Bacillus velezensis 1721 can effectively prevent and treat root rot of peanuts and helps to reduce the usage and residual amounts of chemicals in agricultural products; and the microbial agent has the advantages of safety in use, no residues and convenient use.

Description

technical field [0001] The invention relates to bacillus veles, in particular to a microbial agent for preventing and treating plant diseases. Background technique [0002] With the requirements of intensification and sustainable development of agricultural production, crop diseases and insect pests have become the bottleneck restricting the sustainable development of modern agriculture. Various fungal diseases have caused a large number of crops and commercial crops to reduce or even fail to harvest, causing a lot of losses to the country and people's property. [0003] Peanut, also known as groundnut, belongs to the annual herb of Papilionaceae. Peanuts are known as "vegetable meat", with an oil content of up to 50%, high quality, and a delicate smell. In addition to being edible, it is also used in printing and dyeing and papermaking industries. Peanuts are also a traditional Chinese medicine, suitable for malnutrition, spleen and stomach disorders, cough, phlegm, asthm...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 张珉胡晓敏李春田赵学强苑丽蒲孟肖胡艳霞郎国桥
Owner NORTH CHINA PHARMA GROUP AINO
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