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Manufacturing process of ACE active peptide for reducing blood fat

A manufacturing process and active peptide technology, which is applied in the field of ACE active peptide manufacturing process, can solve the problems of not being able to ensure the purity of active peptides, and achieve the effects of low production cost, low impurity content, and high purity

Inactive Publication Date: 2018-12-07
ANHUI SHUNXIN SHENGYUAN BIOLOGICAL FOOD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current preparation methods of active peptides cannot ensure the purity of active peptides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A manufacturing process of an ACE active peptide for reducing blood fat, comprising the following steps:

[0033] S1. Extract the raw protein, denature the raw protein with strong acid, add trichloroacetic acid to the raw protein for easy acidification, the concentration of trichloroacetic acid is 8%, soak and acidify for 1 hour, and then carry out centrifugal precipitation through a centrifuge and collect precipitation;

[0034] S2. Repeatedly washing the precipitate with clean water, so that the pH of the precipitate in the water is 6-7;

[0035] S3. Add trinitrobenzenesulfonic acid with a concentration of 4% to the precipitate, then use a shaker to shake, react for 1 hour, use a liquid extractor to take out the mixed solution, and then use a high-speed spectrometer to detect trinitrobenzene derivatives in the mixed solution The wavelength of the solution was taken several times until the largest characteristic absorption peak appeared, so that the protein in the pre...

Embodiment 2

[0043] A manufacturing process of an ACE active peptide for reducing blood fat, comprising the following steps:

[0044] S1. Extract the raw protein, denature the raw protein with strong acid, add trichloroacetic acid to the raw protein for easy acidification, the concentration of trichloroacetic acid is 10%, soak and acidify for 1.5h, and then carry out centrifugal precipitation through a centrifuge, collect sediment;

[0045] S2. Repeatedly washing the precipitate with clean water, so that the pH of the precipitate in the water is 6-7;

[0046] S3. Add trinitrobenzenesulfonic acid with a concentration of 5% to the precipitation, then use a shaker to shake, react for 1.5h, use a liquid taker to take out the mixed solution, and then use a high-speed spectrometer to detect the derivative of trinitrobenzene in the mixed solution According to the wavelength of the substance, the liquid was taken and tested several times until the largest characteristic absorption peak appeared, ...

Embodiment 3

[0054] A manufacturing process of an ACE active peptide for reducing blood fat, comprising the following steps:

[0055] S1. Extract the raw protein, denature the raw protein with strong acid, add trichloroacetic acid to the raw protein for easy acidification, the concentration of trichloroacetic acid is 12%, soak and acidify for 2 hours, and then carry out centrifugal precipitation through a centrifuge, collect precipitation;

[0056] S2. Repeatedly washing the precipitate with clean water, so that the pH of the precipitate in the water is 6-7;

[0057]S3. Add trinitrobenzenesulfonic acid with a concentration of 4%-6% to the precipitation, then use a shaker to shake, react for 2 hours, use a liquid extractor to take out the mixed solution, and then use a high-speed spectrometer to detect trinitro in the mixed solution The wavelength of the benzene derivatives is detected by taking liquid several times until the largest characteristic absorption peak appears, so that the prot...

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Abstract

The invention discloses a manufacturing process of an ACE (angiotensin converting enzyme) active peptide for reducing blood fat. The manufacturing process comprises the following steps: (S1) extracting a raw material protein, and denaturing the raw material protein through strong acid; (S2) repeatedly flushing precipitates through clean water; (S3) adding trinitrobenzene sulfonic acid with a concentration of 4%-6% into the precipitates, so as to obtain a peptide solution; (S4) putting the peptide solution into water at 85-100 DEG C, and carrying out water-bath enzyme deactivation; and (S5) centrifuging a mixed solution A by virtue of a centrifugal machine, and carrying out ultrafiltration, so as to obtain clear hydrolysate; (S6) adding a discoloration and odor-removal agent into hydrolysate, and stirring by virtue of a stirring machine, so as to obtain a primary peptide solution; (S7) enabling the primary peptide solution to flow through anion and cation exchange resin for primarily desalination, so as to obtain a fine peptide solution; (S8) carrying out vacuum drying on the fine peptide solution, so as to obtain a finished product; and (S9) detecting the purity of the active peptide in the finished product by virtue of a liquid chromatographic analysis method, so as to obtain the high-purity ACE active peptide.

Description

technical field [0001] The invention relates to the technical field of ACE active peptides, in particular to a manufacturing process of an ACE active peptide for lowering blood lipids. Background technique [0002] ACE is rich in angiotensin-converting enzyme. Peptide is a compound of two or more amino acids connected by peptide bonds. It plays an important physiological role in the human body and exerts physiological functions. Active peptides are called active peptides. ACE active peptide has good solubility and fluidity, fast absorption and high utilization rate in the human body; it can also resist high blood pressure, resist cholesterol, resist thrombosis, improve lipid metabolism, eliminate excess fat, and promote the recovery of red blood cells. Helps recover from fatigue, promotes the absorption of calcium, phosphorus and other trace elements. The current preparation method of active peptide cannot ensure the purity of active peptide. Therefore, we propose a manufa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/48A61P3/06
CPCC12N9/485A61P3/06C12Y304/15001
Inventor 张榆敏
Owner ANHUI SHUNXIN SHENGYUAN BIOLOGICAL FOOD CO LTD
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