Anti-WSSV peptide LvHcS52 from blood blue protein of penaeus vannamei and application thereof

Abstract

The invention relates to an anti-WSSV peptide LvHcS52 from blood blue protein of penaeus vannamei. The amino acid sequence is shown as SEQ ID NO:1; the coding gene is shown as SEQ ID NO:2; the short peptide LvHcS52 can obviously inhibit the WSSV immediate early gene wsv069 and late stage gene wsv421 transcription at both the animal level and the in vitro cell level; meanwhile, the WSSV proliferation can also be obviously inhibited in the body; the STAT signal path in the prawn blood cells can be activated; the transcription of the downstream antimicrobial peptide of the signal path can be triggered so that the anti-WSSV function is achieved. The anti-WSSV peptide LvHcS52 provided by the invention can be used as a preparation for inhibiting WSSV, and can be added into feed as additives so as to improve the anti-WSSV capability of the penaeus vannamei.

Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a short peptide LvHcS52 derived from Litopenaeus vannamei hemocyanin for inhibiting White Spot Syndrome Virus (WSSV), its encoding gene and application. Background technique

[0002] White spot syndrome virus (WSSV) is the most harmful virus in the shrimp farming industry. The virus is highly contagious and has a high lethal rate. After prawns are infected with WSSV, the mortality rate can reach 100% within 7-10 days , has caused huge economic losses to the shrimp farming industry worldwide in the past two decades. It has not been fully controlled so far and has become one of the main obstacles to the development of the shrimp farming industry.

[0003] At present, methods such as cultivating species with strong disease resistance and cutting off the transmission route of WSSV are adopted in the production of prawns to prevent the spread of WSSV disease. At the same time, many scho...

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