Method for producing oil rich in polyunsaturated fatty acids by using lignocellulose

Abstract

Aiming at the problems existing in the prior art, the invention provides a process for preparing oil rich in polyunsaturated fatty acids by fermenting Mortierella alpina with lignocellulosic agricultural and forestry wastes as raw materials. The process includes the steps of pretreatment, saccharification, Mortierella alpina fermentation, oil extraction and the like. The fermentation stage of Mortierella alpina in the present invention adopts the method of gradually lowering the temperature, which can significantly increase the ARA output and reduce the proportion of saturated fatty acids in the oil, and the proportion of ARA in the total oil can reach 48%. Compared with the prior art, the process of the present invention adopts the cellulase preparation based on cellulosome-producing bacteria to realize the saccharification of lignocellulose, which significantly reduces the cost of carbon source and the enzyme cost in the saccharification stage, and the saccharification of cellulose Efficiency is 80%‑90%. In addition, the lignocellulose saccharification stage medium and fermentation medium can be recycled, which can significantly save water and reduce the use of chemicals, and has a significant effect of reducing waste water discharge and reducing costs.

Description

technical field

[0001] The invention relates to the field of microbial fermentation engineering, in particular to a method for cultivating Mortierella alpina by using lignocellulose raw materials to produce oil rich in arachidonic acid and other polyunsaturated fatty acids. Background technique

[0002] Mortierella alapina (Mortierella alapina) is a filamentous fungus of the genus Mortierella in the family Mucorales, which is widely distributed in soil, feces and other decaying organic substances. It is currently the fungus that produces arachidonic acid. The only strain with a formal safety assessment (GRAS). Because it can accumulate a considerable proportion of arachidonic acid (ARA or AA), Mortierella chrysogenum, which belongs to the same genus Mortierella, has received widespread attention and is recognized as an excellent strain for arachidonic acid production. Arachidonic Acid (ARA or AA for short) is an essential omega-6 long-chain polyunsaturated fatty acid that m...

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