Polypyrrole@ferrocene/gold nanoparticle composite material and preparation method and application thereof

Abstract

The invention discloses a polypyrrole@ferrocene / gold nanoparticle composite material. According to the polypyrrole@ferrocene / gold nanoparticle composite material, ferrocene is coated in a polypyrrolenanosphere by adoption of an in-situ polymerization method, and gold nanoparticles are adsorbed on the surface of a polypyrrole-ferrocene composite material by adoption of an electrostatic adsorptionmethod. A preparation method of the polypyrrole@ferrocene / gold nanoparticle composite material comprises the following steps of: 1) preparing the polypyrrole@ferrocene composite material; 2) preparinggold nanoparticle solution; and 3) preparing the polypyrrole@ferrocene / gold nanoparticle composite material. The polypyrrole@ferrocene / gold nanoparticle composite material is used for application ofimpedance type escherichia coli biological sensor modified electrodes, has a linear range, for detecting escherichia coli, of 1*102-1*107 CFU / mL and has a lowest detection limit of 100 CFU / mL. The polypyrrole@ferrocene / gold nanoparticle composite material has the advantages of being simple to operate, low in cost, convenient to use and high in sensitivity, and has huge potential application valuein the field of food safety and clinic analysis.

Description

technical field [0001] The invention relates to a preparation method of a nanocomposite material, specifically a polypyrrole@ferrocene / gold nanoparticle composite material and its preparation method and application. Background technique [0002] The detection of bacteria in drinking water and food, especially the detection of pathogenic bacteria, is of great significance for the control of infectious diseases, the protection of environmental sanitation and the health of the people. Among the various pathogenic bacteria that spread in water and food, E. coli is the most common one. It is a resident bacterium in the intestines of humans and various animals. It is often excreted from humans and animals with feces and widely spread in nature. Human body infected with some Escherichia coli can cause diseases such as peritonitis, hemorrhagic enteritis, cholecystitis, appendicitis and urethritis, and even death in severe cases. Traditional detection methods for Escherichia coli i...

AI Technical Summary

Problems solved by technology

Ampere-type E. coli biosensors usually need to be combined with peroxidase to detect antibodies, and use peroxidase to catalyze hydrogen peroxide to generate electrochemical signals. Many reagents are required and the process is complicated. Therefore, it is urgent to develop a new ampere Detection method of Escherichia coli biosensor

Images