Whitening winkle and scar removal restoration skin care cream and preparation method thereof
A skin cream and scar removal technology, which is applied in skin care preparations, skin diseases, pharmaceutical formulas, etc., to achieve the effect of diluting the color of scars, promoting cell regeneration, and speeding up reconstruction
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Embodiment 1 10
[0039] The preparation of embodiment 1 Panax notoginseng callus culture filtrate
[0040] The notoginseng callus culture filtrate was prepared by the following steps:
[0041] (1) Select the tender leaves of the Panax notoginseng plant as explants for inducing callus, soak them in 70% ethanol solution for 5 minutes on the ultra-clean workbench, then rinse them with sterile water for 4 times, and use sterilized filter paper Blot the surface moisture, cut into small pieces of 0.3cm×0.3cm, inoculate in the medium of MS+1mg / L 2,4-D+1mg / L BA+0.5mg / L TDZ+40g / L sucrose for healing Wounded tissue induction, culture conditions: light control is 6h light and 6h dark alternately; light intensity 1500lx, temperature 25±2°C, culture period is 45 days.
[0042] (2) Take the callus with good growth state obtained in step (1), divide it into mung bean size with sterilized tweezers on the ultra-clean workbench, inoculate it into the subculture medium for cultivation, and subculture The mediu...
Embodiment 2
[0043] The preparation of embodiment 2 rose fruit stem cell extract
[0044] Rosa rose stem cell extract is prepared by the following steps:
[0045] (1) Take the young and tender leaves of Rosa roxburghii, soak them in 70% ethanol solution for 5 minutes on the ultra-clean workbench, then rinse them with sterile water for 4 times, blot the surface moisture with sterilized filter paper, cut into 0.3cm A small piece of ×0.3cm is inoculated in the stem cell induction medium. When inoculating, the back of the leaf is contacted with the medium, and cultured at 27°C for 25 days to obtain stem cells derived from the cambium. The stem cell induction The composition of the medium is: potassium nitrate 2500mg / L, zinc sulfate 2mg / L, inositol 230mg / L, thiamine 21mg / L, L-aspartic acid 145mg / L, L-arginine 160mg / L, glycine 78mg / L, α-naphthaleneacetic acid 2mg / L, sucrose 12mg / L, activated carbon 550mg / L and agar 3.8mg / L, the balance is water, and the pH value is 6.5.
[0046] (2) Transfer t...
Embodiment 3
[0049] Embodiment 3 contains the preparation of bioactive factor liposome
[0050] The formula composition containing bioactive factor liposome, by weight, comprises the components described in the following table:
[0051] Table 1 contains the formula composition of bioactive factor liposome
[0052]
[0053] Preparation steps of formula 1 liposome containing bioactive factors:
[0054] (1) Weigh the soybean lecithin, cholesterol, vitamin E and Tween-80 of formula quantity, mix, add dehydrated alcohol, fully stir and dissolve, obtain solution A;
[0055] (2) Dissolve the extract of Rosa rosea stem cells in a PBS buffer solution with a pH of 7.2, stir until completely dissolved, and make a 0.1 mg / mL solution, then add the prescribed amount of notoginseng callus culture filtrate, and mix well , to obtain solution B;
[0056] (3) Under the continuous stirring of the magnetic stirrer, inject solution B into solution A with a syringe, mix thoroughly for 1 hour, then rotate a...
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