Application of ceratopyran coumarin compound as agricultural fungicide
A technology of pyranocoumarin and compounds, applied in the field of agriculture, can solve the problems of less active research and no agricultural fungicides, etc., and achieve the effects of favorable environmental protection, excellent bactericidal activity, and long-lasting effect
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Embodiment 1
[0012] Example 1: Compound 3', 4'-diacetylkhelactone prevents and treats agricultural diseases
[0013] The tested strain S. sclerotiorum ( Sclerotioia selerotiorum ) is isolated from Sclerotinia sclerotiorum strain; Rhizoctonia solani ( Thanatephorus cucumeris) It is isolated from rice sheath blight diseased leaves; Botrytis cinerea, also known as Botrytis cinerea, is isolated from strawberry gray mold.
[0014] The test compound 3',4'-diacetylkhellactone was dissolved in methanol to make a 1000 μg / mL mother solution, and stored at 4°C for later use. PDA medium: Cut 200g of peeled potatoes into pieces, boil for 10min, take the juice, add 20g of agar, heat until melted, then add 20g of glucose, and dilute to 1000mL with water.
[0015] The inhibition of mycelium growth was measured by the mycelium growth rate assay method, and the bacteria dish (5 mm in diameter) was taken from the edge of the pre-cultivated test bacteria on the PDA plate, and the mother liquor of the test ...
Embodiment 2
[0020] Weigh the compound and conventional auxiliary materials such as lignin and diatomaceous earth according to the conventional process to prepare a wettable powder with an active ingredient concentration of 2%.
Embodiment 3
[0022] Weigh the compound and conventional auxiliary materials such as calcium dodecylbenzenesulfonate, styrene-ethylphenol polyoxyethylene ether, solvent oil, etc., and make an emulsifiable concentrate with an active ingredient concentration of 2% according to a conventional process.
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