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Method for separating and extracting new compound LW-1 from secondary metabolites of aspergillus terreus

A secondary metabolite, LW-1 technology, applied in the biological field, can solve the problem of not obvious inhibitory activity of Gram-negative bacteria, and achieve the effect of accelerating the release of intracellular substances and improving the extraction rate

Inactive Publication Date: 2018-12-21
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The isolated compound has a strong inhibitory effect on methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus (VRE), and has a certain inhibitory effect on Gram-negative bacteria. The minimum inhibitory concentration MIC is less than 12.5μM, indicating that it can be developed into a potential antibacterial drug, but the inhibitory activity of the fungal secondary metabolite derivatives from Liushan Lake on Gram-negative bacteria is not obvious

Method used

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  • Method for separating and extracting new compound LW-1 from secondary metabolites of aspergillus terreus
  • Method for separating and extracting new compound LW-1 from secondary metabolites of aspergillus terreus
  • Method for separating and extracting new compound LW-1 from secondary metabolites of aspergillus terreus

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Embodiment 1

[0021] A new compound LW-1 isolated from secondary metabolites of Aspergillus terreus is a compound represented by formula I, its stereoisomer or a pharmaceutically accessible salt, and formula I has the following structure:

[0022]

[0023] A method for preparing a new compound LW-1 isolated from secondary metabolites of Aspergillus terreus, comprising spore preparation, strain fermentation, secondary metabolite extraction and separation and purification, the specific steps are:

[0024] Spore preparation: put the glycerin cryopreservation tube of Aspergillus terreus (PDA liquid freezing solution freshly prepared with 20% glycerol and 3.3% seawater) stored in a -80°C ultra-low temperature refrigerator in a clean operating table, recover for 10 minutes, and burn Dip the sterilized inoculation loop into the resuscitated cryopreservation solution, and evenly streak in the freshly prepared PDA slant solid medium. Then, place the slant medium in a constant temperature incubator...

Embodiment 2

[0030] A new compound LW-1 isolated and extracted from secondary metabolites of Aspergillus terreus is a compound represented by formula I, its stereoisomer or a pharmaceutically accessible salt, characterized in that formula I has the following structure:

[0031]

[0032] A method for preparing a new compound LW-1 isolated from secondary metabolites of Aspergillus terreus, comprising spore preparation, strain fermentation, secondary metabolite extraction and separation and purification, the specific steps are:

[0033]Spore preparation: put the glycerin cryopreservation tube of Aspergillus terreus (PDA liquid freezing solution freshly prepared with 20% glycerol and 3.3% seawater) stored in a -80°C ultra-low temperature refrigerator in a clean operating table, recover for 10 minutes, and burn Dip the sterilized inoculation loop into the resuscitated cryopreservation solution, and evenly streak in the freshly prepared PDA slant solid medium. Then, place the slant medium in a...

Embodiment 3

[0038] A new compound LW-1 isolated and extracted from secondary metabolites of Aspergillus terreus is a compound represented by formula I, its stereoisomer or a pharmaceutically accessible salt, characterized in that formula I has the following structure:

[0039]

[0040] A method for preparing a new compound LW-1 isolated from secondary metabolites of Aspergillus terreus, comprising spore preparation, strain fermentation, secondary metabolite extraction and separation and purification, the specific steps are:

[0041] Spore preparation: put the glycerin cryopreservation tube of Aspergillus terreus (PDA liquid freezing solution freshly prepared with 20% glycerol and 3.3% seawater) stored in a -80°C ultra-low temperature refrigerator in a clean operating table, recover for 10 minutes, and burn Dip the sterilized inoculation loop into the resuscitated cryopreservation solution, and evenly streak in the freshly prepared PDA slant solid medium. Then, place the slant medium in ...

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Abstract

The invention discloses a method for separating and extracting a new compound LW-1 from secondary metabolites of aspergillus terreus. The method comprises the steps of spore preparation, strain fermentation, secondary metabolite extraction as well as separation and purification, wherein the strain fermentation comprises the following steps: adding an epigenetic modification regulator SAHA, paricalcitol and triglyceride into a fungus culture medium, stirring uniformly, standing, and carrying out fermentation culture. The method provided by the invention has the beneficial effects that the paricalcitol and the triglyceride are added into the culture medium, and realize collaborative gain with the epigenetic modification regulator SAHA, so that epigenetic modification is performed on the aspergillus terreus, the aspergillus terreus is promoted to generate the diverse secondary metabolites, and the new secondary metabolite LW-1 is accordingly obtained by means of separation; the new secondary metabolite LW-1, obtained by using the method, of the aspergillus terreus as well as stereoisomers or pharmaceutically acceptable salts of the new secondary metabolite LW-1 have long-acting antibacterial and anti-inflammatory effects.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a new compound LW-1 isolated and extracted from secondary metabolites of Aspergillus terreus. Background technique [0002] As we all know, microorganisms have long been an important source of active substances such as antibiotics and enzyme inhibitors. Microorganisms have become a hotspot in the field of natural product research due to their large-scale fermentation and cultivation, no raw material restrictions, and no environmental damage. In recent years, the probability of obtaining new compounds from terrestrial microorganisms has been greatly reduced. However, due to the specific living environment of marine-derived microorganisms, in addition to producing metabolites similar to those on land, sometimes some new compounds with specific structures can also be produced, showing a huge The prospect of development and utilization has aroused extensive attention of scholars in the ...

Claims

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Application Information

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IPC IPC(8): C07D241/18C12P17/12A61P29/00C12R1/66
CPCA61P29/00C07D241/18C12P17/12
Inventor 孙坤来刘文王斌初学梅梁丽丽陈荫赵玉勤
Owner ZHEJIANG OCEAN UNIV
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