Method for purifying mupirocin

A purification method and mupirocin technology, applied in the field of drug synthesis, can solve the problems of non-compliance with GMP production requirements, difficulty in large-scale industrial production, and high production costs, and meet the needs of large-scale production, with high production efficiency and low cost. Effect

Active Publication Date: 2018-12-21
福建康鸿生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Adopt this method to be loaded down with trivial details process, complicated operation, high production cost, and does not meet the GMP production requirement, it is difficult to carry out large-scale industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The invention provides an embodiment of a purification method of mupirocin.

[0029] A method for purifying mupirocin, comprising the following steps:

[0030] 1) Membrane filtration of the fermentation liquid: a ceramic membrane with a pore size of 50 nm was used to separate the mupirocin fermentation liquid through membrane filtration to obtain a filtrate with a filtration yield of 95%.

[0031] 2) Filtrate membrane concentration: at 0-5°C, the filtrate obtained in step 1) is membrane-concentrated with a molecular weight 200 Dalton nanofiltration membrane, and concentrated until the content of mupirocin in the concentrated solution is 50000-60000ug / mL ;

[0032] 3) Solvent extraction: use butyl acetate to extract the concentrated solution obtained in step 2) to obtain an extract containing mupirocin; the total added volume of the butyl acetate is 1 times the volume of the concentrated solution, and the extraction yield 98%.

[0033] 4) Dehydration with dehydrating ...

Embodiment 2

[0038] A method for purifying mupirocin, comprising the following steps:

[0039] 1) Membrane filtration of the fermentation liquid: the mupirocin fermentation liquid was subjected to membrane filtration for solid-liquid separation with an ultrafiltration membrane to obtain a filtrate with a filtration yield of 98%.

[0040] 2) Filtrate membrane concentration: at 25-30°C, the filtrate obtained in step 1) is membrane-concentrated with a reverse osmosis membrane until the content of mupirocin in the concentrated solution is 45000-55000ug / mL;

[0041] 3) Solvent extraction: use butyl acetate to extract the concentrated solution obtained in step 2) to obtain an extract containing mupirocin; the total added volume of the butyl acetate is 2 times the volume of the concentrated solution, and the extraction yield 99%.

[0042] 4) Dehydration with dehydrating agent: add dehydrating agent calcium sulfate to the extract obtained in step 3) for dehydration, and the added weight of the de...

Embodiment 3

[0047] A method for purifying mupirocin, comprising the following steps:

[0048] 1) Membrane filtration of the fermentation liquid: the mupirocin fermentation liquid was subjected to membrane filtration for solid-liquid separation using a hollow fiber membrane to obtain a filtrate with a filtration yield of 96%.

[0049] 2) Filtrate Membrane Concentration: At 10-15°C, the filtrate obtained in step 1) is membrane-concentrated with a molecular weight 200 Dalton nanofiltration membrane until the content of mupirocin in the concentrated solution is 40000-50000ug / mL ;

[0050] 3) Solvent extraction: the concentrated solution obtained in step 2) is extracted with a mixed solvent of ethyl acetate and butyl acetate at a volume ratio of 1:1 to obtain an extract containing mupirocin; the extracted organic solvent The total volume added is 3 times the volume of the concentrate, and the extraction yield is 99%.

[0051]4) dehydrating agent dehydration: adding dehydrating agent calcium ...

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Abstract

The invention discloses a method for purifying mupirocin. The method comprises the following steps: filtering with a fermentation broth membrane; carrying out solid-liquid separation on a mupirocin fermentation broth through membrane filtration so as to obtain filtrate; carrying out membrane concentration on the filtrate, namely carrying out membrane concentration on the filtrate obtained in the step 1) till the content of mupirocin in the concentrated liquid is 40000-60000mu g / mL; further carrying out extraction, dehydration, concentration and crystallization, thereby obtaining the mupirocin.By adopting the method disclosed by the invention, large-scale industrial production of the mupirocin can be achieved, GMP (Good Manufacturing Practice) production requirements can be met, conventional production procedures can be simplified, the production efficiency can be improved, and environmental protection is realized and pollution can be reduced.

Description

technical field [0001] The invention relates to the field of drug synthesis, in particular to a method for purifying mupirocin. Background technique [0002] Mupirocin, also known as pseudomonic acid A, is a gram-positive bacteria (such as Staphylococcus aureus, Streptococcus pyogenes, Streptococcus pneumoniae) pneumoniae), Klebsiella pneumoniae) and some Gram-negative bacteria (such as Haemophilus influenzae, Neisseria gonorrhoeae) have growth-inhibitory antibiotics [A.Ward, D.M. Campoli-Richards, Drugs 32, 425-444 (1986)]. By inhibiting isoleucine-tRNA synthase, it affects the peptide synthesis of pathogenic bacteria. An advantageous feature of this antibiotic is that it has very low toxicity to humans and animals and it is negative in the Ames (Ames) test. At present, mupirocin is mostly used in human therapy to treat skin infections (such as pustules) pyoderma), nasal and outer ear infections, acne, burns, eczema, psoriasis, in ulcerative conditions for the treatment ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D407/06
CPCC07D407/06
Inventor 张茂华蒋永飞赖庆云赵正国陈亮
Owner 福建康鸿生物科技有限公司
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