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Compound biological ink and preparation method thereof

A bio-ink and bioactive molecule technology, applied in the field of composite bio-ink and its preparation, can solve the problems of low mechanical strength, unfavorable application, poor mechanical and structural stability of decellularized matrix hydrogel, etc., and achieve good bioactivity and reliability Effects of 3D printing properties

Inactive Publication Date: 2018-12-21
THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, related studies including the inventors of this application have shown that the mechanical strength of a single decellularized matrix hydrogel is still small enough to be self-supporting to realize the construction of a 3D model
At the same time, its gelation is achieved through the self-assembly of molecules, and its mechanical and structural stability is poor, which is not conducive to long-term application in vivo and in vitro.

Method used

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  • Compound biological ink and preparation method thereof
  • Compound biological ink and preparation method thereof
  • Compound biological ink and preparation method thereof

Examples

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Effect test

Embodiment 1

[0040] A kind of embodiment of composite biological ink of the present invention, its preparation raw material comprises the component of following mass percent concentration: acellular matrix hydrogel 1%, PLGA-PEG-PLGA 30%, factor is β-NGF (final concentration 60ng / ml), the balance is water.

[0041] The preparation method of above-mentioned composite biological ink comprises the steps:

[0042] (1) Preparation of acellular matrix hydrogel: after removing pig heart adipose tissue and connective tissue, use 1% trion x-100 aqueous solution, 7% sodium deoxycholate aqueous solution to repeatedly process successively to obtain acellular tissue; freeze-dry The decellularized tissue is then pulverized, treated with pepsin to obtain a viscous gel solution, then adjusted to pH (7.5) and ionic strength at 2°C, and heated to 39°C to obtain the decellularized matrix hydrogel, and the final concentration is controlled 1%.

[0043] (2) Quantitative PLGA-PEG-PLGA and β-NGF were dissolved ...

Embodiment 2

[0045] A kind of embodiment of composite bio-ink of the present invention, its preparation raw material comprises the component of following mass percent concentration: decellularized matrix hydrogel 1%, double-bond modified hyaluronic acid 2%, factor is VEGF (final concentration 40ng / ml), the balance is water.

[0046] The preparation method of above-mentioned composite biological ink comprises the steps:

[0047] (1) Preparation of acellular matrix hydrogel: After removing porcine placental adipose tissue and connective tissue, use 2% trionx-100 aqueous solution and 5.5% sodium deoxycholate aqueous solution to repeatedly process successively to obtain acellular tissue; The decellularized tissue is then pulverized, treated with pepsin to obtain a viscous gel solution, then adjusted to pH (7.9) and ionic strength at 3°C, and heated to 38°C to obtain the acellular matrix hydrogel, and the final concentration is controlled as 1%.

[0048] (2) Quantitative double-bond-modified ...

Embodiment 3

[0050] A kind of embodiment of composite biological ink of the present invention, its preparation raw material comprises the component of following mass percentage concentration: acellular matrix hydrogel 2%, double bond modified gelatin 8%, factor is BDNF (final concentration is 20ng / ml), the balance is water.

[0051] The preparation method of above-mentioned composite biological ink comprises the steps:

[0052] (1) Preparation of acellular matrix hydrogel: After removing pig heart adipose tissue and connective tissue, use 3% trion x-100 aqueous solution, 4% sodium deoxycholate aqueous solution to repeatedly process successively to obtain acellular tissue; freeze-dry The decellularized tissue is then pulverized, treated with pepsin to obtain a viscous gel solution, then adjusted to pH (7) and ionic strength at 4°C, and heated to 37°C to obtain the decellularized matrix hydrogel, and the final concentration is controlled 2%.

[0053] (2) Quantitative double-bond-modified ...

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Abstract

The invention discloses compound biological ink. Preparation raw materials of the compound biological ink comprise acellular dermis matrix hydrogel, a crosslinking curing agent and a bioactive molecule; the invention further discloses a preparation method of the compound biological ink; the preparation method comprises the following steps: (1) preparing the acellular dermis matrix hydrogel: afterremoving target tissues or fat tissues and connective tissues of organs, repeatedly treating with a 1 to 5 percent trion x-100 water solution and a 1.5 to 7 percent sodium deoxycholate water solutionfor a plurality of times in sequence, so as to obtain acellular tissues; freezing and drying the acellular tissues; then crushing and treating through pepsin to obtain a sticky gel solution; then regulating the pH (Potential of Hydrogen) and ion strength at 2 to 8 DEG C; raising the temperature to 33 to 39 DEG C to obtain the acellular dermis matrix hydrogel; (2) adding the crosslinking curing agent, an active factor and a short peptide, and compounding at 1 to 8 DEG C to obtain the compound biological ink. The compound biological ink prepared by the method disclosed by the invention has goodbioactivity and a 3D (Three Dimensional) printable property.

Description

technical field [0001] The invention relates to the technical field of 3D printing, in particular to a composite biological ink and a preparation method thereof. Background technique [0002] Biological 3D printing technology has the characteristics of high precision, high efficiency, and personalized manufacturing. It has attracted enough attention in the field of biomedicine and has a very broad application prospect. The core technology of bio-3D printing is the three-dimensional controlled assembly process of cells. According to the digital model of organ anatomy, by controlling the 3D assembly of individual cells and cell clusters, it can be integrated into the human body's metabolic system for repairing and replacing lesions. Fabrication of artificial organs of tissues and organs. At present, bio-3D printing has made some progress in the reconstruction of structural tissues including teeth, bones, and cartilage. [0003] So far, the use of 3D printing technology to ma...

Claims

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Application Information

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IPC IPC(8): C09D11/04C09D11/104C09D11/14B33Y70/00
CPCB33Y70/00C09D11/04C09D11/104C09D11/14
Inventor 王涛朱庆棠全大萍刘小林吴泽佳闫立伟
Owner THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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