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High-density enriching method of autotrophic nitrifying bacterial consortium

A technology of nitrifying bacteria and nitrosifying bacteria, which is applied in chemical instruments and methods, bacteria, biological water/sewage treatment, etc., can solve the problems of automatic supplementation and the impact of carbon source supplementation, and achieve The effect of improving nitrification ability and solving ammonia nitrogen problem

Active Publication Date: 2018-12-21
SHENYANG RES INST OF CHEM IND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this type of problem has not been well solved in the prior art, especially in the supplementary process of inorganic carbon sources, the impact of supplementary carbon sources on the pH of the system has not been well resolved, and in the supplementary process of inorganic nitrogen sources, there is no Realize automatic replenishment

Method used

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  • High-density enriching method of autotrophic nitrifying bacterial consortium

Examples

Experimental program
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Effect test

Embodiment 1

[0030] The reaction device includes a fermenter, a carbon source storage tank, a peristaltic pump A, a pH electrode, a control system, a nitrogen source storage tank, a peristaltic pump B, a dissolved oxygen electrode, a temperature probe, a stirring motor, an air compressor, and a heater. The fermenter is inserted with a pH electrode, a dissolved oxygen electrode and a temperature probe, and the pH electrode, the dissolved oxygen electrode and the temperature probe are respectively connected to the control system through wires, and the fermenter is respectively connected to the external mixer, peristaltic pump A, and peristaltic pump B through wires. , the air compressor and the heater are connected, and the peristaltic pump A, peristaltic pump B, air compressor and heater are respectively connected to the control system through wires. In addition, the peristaltic pump A is connected to the carbon source storage tank and the peristaltic pump through pipelines. B passes through...

Embodiment 2

[0038] When Na 2 CO 3 When the cumulative amount X value of the solution consumption reaches V / 4 (L), the expansion is over, and the nitrification rate of the expansion solution is > 160 mg (NO 3 -N) / L / h. Stop stirring and stand still for 1 hour to collect the autotrophic nitrifying bacteria consortium, and the nitrification rate per unit mass of the harvested consortium is >600 mg (NO 3 -N) / g / h. Example 2 100L-scale automatic expansion of nitrosifying bacteria and nitrifying bacteria copolymers

[0039] The volume of the fermenter is 100L, the consortium of nitrosifying bacteria and nitrifying bacteria is used as the inoculum, the inoculum size is 1%, and the initial volume of the medium is V=80L. Stirring speed is 220r / min, keeps constant in the expansion cultivation process, and the inorganic carbon source is 10% Na 2 CO 3 solution, the inorganic nitrogen source is 20% NH 4 Cl solution. During the expansion process, the dissolved oxygen DO was controlled at 20%, and...

Embodiment 3

[0047] Example 3: Automatic enrichment and expansion of 100L-scale activated sludge autotrophic nitrifying bacteria flora

[0048] The volume of the fermenter is 100L, the activated sludge of the pesticide wastewater biochemical pool is used as the inoculum, the inoculum size is 10%, and the initial volume of the medium is V=80L. Stirring speed is 220r / min, keeps constant in the expansion cultivation process, and the inorganic carbon source is 10% Na 2 CO 3 solution, the inorganic nitrogen source is 20% NH 4 Cl solution. During the expansion process, the dissolved oxygen DO was controlled at 20%, and the pH was controlled at 7.50. Among them, the initial medium of autotrophic nitrifying bacteria per liter of Na in water 2 CO 3 2g-4g, MgSO 4 0.2g-0.4g, KCl 0.2g-0.4g, phosphoric acid to adjust the pH between 7.5-8.0, NH 4 Cl 0.5g-1.0g, initial NH 4 -N concentration 150mg / L-300mg / L, NO in the initial medium 3 -N concentration is controlled at 0mg / L-10mg / L.

[0049] Du...

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Abstract

The invention belongs to the field of microbial fermentation and specifically relates to a high-density enriching method of an autotrophic nitrifying bacterial consortium and application of the obtained autotrophic nitrifying bacterial consortium to sewage treatment. The high-density enriching method of the autotrophic nitrifying bacterial consortium comprises the steps of inoculating thalli in anautotrophic nitrifying bacteria initial culture medium; culturing the thalli under a stirring condition by controlling the dissolved oxygen (DO) at 20-40%, at the temperature of 28-32 DEG C and the pH value of 7.5-8.0; according to a relation between NO3-N cumulated amount and inorganic carbon source consumption in the culturing process, monitoring the change of pH value in the culturing processby a pH electrode to supplement an inorganic nitrogen source and the inorganic carbon source to a culture system, and controlling the ammonia nitrogen concentration in the culturing process at 150-300mg / L; and enabling the pH value of the system to be 7.5-8.0 by the supplemented carbon source to further realize high-density automatic propagation of the autotrophic nitrifying bacterial consortium.The high-density enriching method of the autotrophic nitrifying bacterial consortium, provided by the invention, has the advantage that a problem of the consortium of poor ammonia-nitrogen treating effect caused by reasons, such as poor strain adaptation ability, added strain loss, insufficient added strain number and high cost, in the ammonia-nitrogen waste water treatment application process iseffectively solved.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a high-density enrichment method of an autotrophic nitrifying bacteria consortium and the application of the obtained high-density autotrophic nitrifying bacteria consortium to sewage treatment. Background technique [0002] Autotrophic nitrifying bacteria are collectively referred to as nitrosifying bacteria and nitrifying bacteria consortia. These microorganisms can convert ammonia nitrogen into nitrite nitrogen and then into nitrate nitrogen, and play an important role in the natural N element cycle. Along with the development of microorganism separation technology, this kind of microorganism is applied in various fields, as patent CN101039692A discloses a kind of consortium composed of Nitrosomonaseutropea and Nitrobacter victoria in aquaculture. Subsequently, this type of consortium was widely used in the treatment of ammonia nitrogen wastewater, but the app...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34
CPCC12N1/20C02F3/34
Inventor 杨德玉安鹏赵彭年方广君王加友朱婧涵丁一凡
Owner SHENYANG RES INST OF CHEM IND
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