Preparation method of cinnamaldehyde essential oil liposome antibacterial double-layer membrane with regulated releasing
A technology of oil liposome and cinnamaldehyde essence, which is applied in the field of food antibacterial packaging, can solve the problems of inability to achieve antibacterial packaging and the like, and achieve the effect of improving mechanical strength
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[0044] Example 1:
[0045] 1. Preparation of heat-sensitive liposomes
[0046] (1) Dissolve 0.4g dipalmitoylphosphatidylglycerol, 0.2g distearoylphosphatidylcholine, and 0.2g cholesterol in 10mL of chloroform, add 200μl of cinnamaldehyde essential oil, and dissolve it with ultrasound, then pour it into a circle Bottom flask.
[0047] (2) Place the round-bottom flask in a rotary evaporator, set the temperature of the rotary evaporator to 40°C, and evaporate the solvent by rotary evaporation under reduced pressure of 0.08MPa for 40 minutes. The inner wall of the round-bottom flask has a honeycomb film.
[0048] Add 0.1 Mm of PBS buffer solution to the round-bottomed flask, hydrate and spin-evaporate at 0.02MPa for 40 minutes to obtain a hydrated solution.
[0049] (3) The resulting hydrated solution was sonicated in a closed probe ultrasonic instrument at 400W for 10 minutes, and centrifuged, and a 0.22 μm filter membrane was passed through the membrane to obtain heat-sensitive liposomes...
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