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A copper metal-organic framework-mimicking enzyme material and its preparation and application

An organic skeleton and enzyme-mimicking technology, applied in the field of enzyme-mimicking, can solve problems such as impeding the promotion and application of natural enzymes, difficulties in the application of natural enzymes, high cost, etc., and achieve excellent catalytic effects, intuitive and obvious effects, and low-cost effects

Active Publication Date: 2021-06-22
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, natural enzymes also have their limitations
Natural enzymes are produced by living cells, so their development must be extracted and purified, but the extraction and purification of natural enzymes is not only difficult but also expensive, which greatly hinders the popularization and application of natural enzymes
At the same time, since most of the natural enzymes are proteins, they are easily inactivated by the interference of environmental temperature, pH, organic reagents and other factors during use, which makes the application of natural enzymes more difficult.

Method used

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  • A copper metal-organic framework-mimicking enzyme material and its preparation and application
  • A copper metal-organic framework-mimicking enzyme material and its preparation and application
  • A copper metal-organic framework-mimicking enzyme material and its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Preparation of sheet-like Cu-MOFs mimic enzyme material

[0028] 1) Add 0.2mmol copper nitrate to 0.5mL N, N-dimethylformamide, and obtain 0.4mol / L solution A after magnetic stirring; add 0.2mmol 5-nitroisophthalic acid to 0.5mL N , in N-dimethylformamide, after magnetic stirring, 0.4mol / L solution B was obtained; solution A and 4mL methanol were added to solution B under stirring conditions, stirred for 20-40 minutes and then placed in a reaction kettle, At 75°C, react at a constant temperature for 48 hours; after the reaction is completed and cooled to room temperature, centrifuge and wash at a speed of 5000rpm, and then dry at 60°C for 24 hours to obtain a sheet-like Cu-MOFs mimic enzyme material (see figure 1 ). 4 mg of this material was dispersed into 20 mL of deionized water to obtain a 0.2 mg / mL Cu-MOFs mimic enzyme aqueous solution.

Embodiment 2

[0029] Example 2 Preparation of sheet Cu-MOFs mimic enzyme material

[0030] The difference from Example 1 is that the amounts of copper nitrate and 5-nitroisophthalic acid are both changed to 0.6 mmol. Add 0.6mmol copper nitrate to 0.5mL N,N-dimethylformamide, and obtain 1.2mol / L solution A after magnetic stirring; add 0.6mmol 5-nitroisophthalic acid to 0.5mL N,N - In dimethylformamide, after magnetic stirring, a solution B of 1.2 mol / L was obtained; solution A and 4 mL of methanol were added to solution B under stirring, and placed in a reaction kettle after stirring for 20 to 40 minutes. At 75°C, react at a constant temperature for 48 hours; after the reaction is completed and cooled to room temperature, centrifuge and wash at a speed of 5000 rpm, and then dry at 60°C for 24 hours to obtain a sheet-like Cu-MOFs simulation material, whose shape and size are the same as Embodiment 1 is consistent.

Embodiment 3

[0031] Example 3 To investigate the catalytic activity of the Cu-MOFs mimic enzyme material as a peroxide mimic enzyme, the following operations were performed:

[0032]Add 200 μL 0.2 mg / mL Cu-MOFs simulated enzyme solution, 200 μL pH=7.0 MOPS buffer solution, 200 μL 5 mM 3,3′,5,5′-tetramethylbenzidine, 80 μL 100 mM H 2 o 2 Solution and deionized water, so that the reaction system was 2mL, and solution C was obtained; 200μL buffer solution with pH=7.0, 200μL 5mM 3,3′,5,5′-tetramethylbenzidine solution, 80μL 100mM H 2 o 2 Solution and deionized water, so that the reaction system is 2mL, to obtain solution D; after reacting at 20°C for 5min, use a UV-visible spectrophotometer to detect the absorption spectra of solutions C and D respectively at 500-800 (see figure 2 ).

[0033] attached figure 2 It is shown that the sheet-like Cu-MOFs mimic enzyme material has peroxide mimic enzyme activity and has good catalytic performance.

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Abstract

The invention discloses a copper metal organic framework (Cu-MOFs) simulated enzyme material and its preparation and application. Its nano-morphology is flake. The Cu-MOFs simulated enzyme material can be prepared by the following method: dissolving copper nitrate in N,N-dimethylformamide to obtain solution A, dissolving 5-nitroisophthalic acid in N,N-dimethylformamide Amide to obtain solution B; add solution A and methanol to solution B under stirring conditions, stir for 20 to 40 minutes, place in a reaction kettle, and react at a constant temperature for 48 to 72 hours at 70 to 80°C; after the reaction, Centrifuge and wash, and then dry at 60°C for 12 to 24 hours to obtain a sheet-like Cu-MOFs simulated enzyme material, which is dispersed in water to obtain a Cu-MOFs simulated enzyme solution. The material has excellent peroxide mimetic enzyme activity, and has the advantages of simple preparation process, low cost, good applicability and the like compared with natural horseradish peroxidase (HRP). This material can be used as a substitute for HRP, and has potential application value in biological detection, immunoassay, clinical diagnosis, etc.

Description

technical field [0001] The invention relates to the field of simulated enzymes, in particular to a copper metal organic framework simulated enzyme material and its preparation method and application. Background technique [0002] Natural enzymes are macromolecular substances widely present in organism cells, most of which are composed of proteins (a few are RNA). Natural enzymes have the advantages of high catalytic efficiency and specificity, play a vital role in the metabolic process of living organisms, and participate in vital activities such as respiration, digestion, and exercise, so they have attracted extensive attention from researchers. However, natural enzymes also have their limitations. Natural enzymes are produced by living cells, so their development must be extracted and purified, but the extraction and purification of natural enzymes is not only difficult but also expensive, which greatly hinders the popularization and application of natural enzymes . At ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J31/22B01J37/10B82Y40/00G01N21/31
CPCB01J31/1691B01J31/2213B01J37/10B82Y40/00G01N21/31
Inventor 汪嘉炎李文英郑岳青
Owner NINGBO UNIV
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