Green fluorescent protein-based nanoparticle, preparation method and application thereof in cell imaging and cell nucleus nucleolar staining

A green fluorescent protein and nanoparticle technology, applied in the field of fluorescent protein-based nanomaterials, can solve complex, expensive, and not commercially available problems, and achieve good biocompatibility, no-cleaning imaging, and cost-effective effects
CN109096384AActive Publication Date: 2018-12-28JILIN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JILIN UNIV
Publication Date
2018-12-28

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Abstract

A green fluorescent protein-based nanoparticle, a preparation method and an application thereof in cell imaging and cell nucleus nucleolar staining belong to the field of fluorescent protein-based nanomaterials. The method includes the steps of adding a concentration thioflavin-T aqueous solution as a building molecule in deionized water, adding a bovine serum albumin or human serum albumin aqueous solution, heating the solution and finally cooling the solution to the room temperature to obtain a green fluorescent protein-based nanoparticle T-FPNs solution. An experiment with Hela cells in yeast cells shows that T-FPNs can be used as bioimaging materials and nucleolar localization dyes. The nanoparticle is suitable for large-scale production because a synthesis method is cost-effective, environmentally friendly, simple and biocompatible. The T-FPNs with excellent light stability can stain nucleoli of live cells and fixed cells nucleoli for fast real-time no-clean imaging.
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Description

Technical field

[0001] The invention belongs to the field of fluorescent protein-based nanomaterials, and specifically relates to a green fluorescent protein-based nanoparticle, a preparation method and application thereof in cell imaging and nucleolar staining. Background technique

[0002] In recent years, various nanomaterials with different functions and properties have made great progress in the design and manufacture. Most of the developed nanomaterials (semiconductor quantum dots, fluorescent carbon dots, luminescent silicon nanoparticles, organic small molecules and organic fluorescent nanoparticles, etc.) are used in bioimaging, drug delivery and other medical diagnosis and treatment applications. But at the same time, the application of these materials has been restricted to varying degrees, such as lack of critical biocompatibility, high toxicity to organisms, complex synthesis routes, high costs, and difficulty in comprehensive promotion.

[0003] The nucleolus of the ...

Claims

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