Avian adenovirus, quadruple vaccine and preparation method thereof

An avian adenovirus, avian influenza virus technology, applied in the direction of biochemical equipment and methods, viruses, vaccines, etc., can solve the problems of economic loss, harm, severe immunosuppression, etc., achieve small immune dose, rapid antibody production, immunogenicity Good results

Active Publication Date: 2018-12-28
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Chicken infectious bursal disease is an acute and highly contagious infectio

Method used

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  • Avian adenovirus, quadruple vaccine and preparation method thereof
  • Avian adenovirus, quadruple vaccine and preparation method thereof
  • Avian adenovirus, quadruple vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Screening of Antigen Strain (I Avian Adenovirus Group I Serum 4DC Strain) Used for Seedling Production

[0029] In 2015, liver disease material was taken from a dead chicken in a chicken farm in Dachang, Hebei (necropsy showed hepatomegaly, necrosis, and a large amount of yellow fluid in the pericardium), and was inoculated with the yolk sac route for 6 days after grinding, homogenizing, centrifuging, and filtering Age SPF chicken embryos were used for virus isolation. Chicken embryos were all dead 96 hours after inoculation with the disease material, and the embryo body of the chicken embryos had dysplasia, congestion and hemorrhage, liver mottled necrosis and other lesions. The dead embryos were harvested and homogenized with saline at 1:3 to make a virus suspension. The virus suspension continued to be passed on chicken embryos for 2 times, and then inoculated with LMH cells for 3 generations to obtain an isolated virus strain adapted to the growth of LMH c...

Embodiment 2

[0032] Embodiment 2 Preparation and inspection of chicken Newcastle disease, avian influenza, infectious bursal and avian adenovirus quadruple vaccine

[0033] 1. Preparation of Antigen Solution for Seedling Production

[0034] 1.1 Preparation of avian influenza (H9 subtype) antigen solution

[0035] 1.1.1 Avian influenza (H9 subtype) BX13 strain was isolated and preserved by the Animal Husbandry and Veterinary Research Institute of Beijing Academy of Agriculture and Forestry Sciences in 2013 from a sick chicken flock with H9 subtype avian influenza in Beijing. After systematic identification, it was proved to be H9N2 subtype type of avian influenza virus. The propagation of virus gets the poison seed for production, and makes appropriate dilution (such as 10 -3 to 10 -5diluted), inoculate 0.1 mL into the allantoic cavity of each embryo, seal the pinhole, and continue to incubate at 36-37°C without turning the eggs. Incubation and observation Within 48 hours after inoculat...

Embodiment 3

[0087] 1. Preparation of inactivated vaccine with oil adjuvant

[0088] 1. Oil phase preparation

[0089] Take 94 parts by weight of white oil for injection, add 2% (weight ratio) of aluminum stearate, heat, stir while adding, until completely transparent, then add 6 parts by weight of Siben-80, after mixing, autoclave spare.

[0090] 2. Aqueous phase preparation

[0091] Mix the concentrated and inactivated NDV, AIV, IBDV and FADV virus liquid in an appropriate ratio (1:1:3:1), so that the NDV antigen content in each final product is ≥10 8.2 EID 50 、AIV antigen content ≥10 8.2 EID 50 、IBDV antigen content ≥10 8.2 TCID 50 、FADV antigen content ≥10 7.2 TCID 50 Take 96 parts by weight of the mixed antigen solution, add 4 parts by weight of sterilized Tween-80, and shake well until the Tween-80 is completely dissolved.

[0092] 3. Emulsification

[0093] Pour 2 parts by weight of the oil phase into the emulsification tank, stir slowly while slowly adding 1 part of the ...

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Abstract

The invention provides a novel avian adenovirus, a quadruple vaccine and a preparation method thereof. The novel avian adenovirus is avian adenovirus group I serum type 4 DC strain, which was preserved in General Microbiology Center of China Microbe Preservation Management Committee on April 25, 2018 and was assigned the accession number CGMCC No. 15589; the quadruple vaccine is a quadruple vaccine against Newcastle disease, avian influenza, infectious bursal disease and group I type 4 aviadenovirus DC strain. The quadruple vaccine has good immunogenicity, the antibody production is quick after immunity, the generated antibody titer is high, the maintenance time is long, the immunizing dose is small, a selected adjuvant is easy to inject, and four diseases can be prevented and controlled with one needle. The vaccine has the advantages of being efficient and good in safety.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a quadruple vaccine of avian adenovirus, a new type of Newcastle disease, avian influenza (H9 subtype), infectious bursa and avian adenovirus and a preparation method thereof. Background technique [0002] Newcastle disease is caused by Newcastle disease virus, a highly contagious and acute septicemia infectious disease of poultry characterized by respiratory and gastrointestinal mucous membrane bleeding. Simultaneous infection with other pathogens such as H9 subtype avian influenza can cause endogenous infection, which will reduce the egg production of laying hens and increase the mortality of breeding chickens. It is one of the main diseases that endanger my country's poultry industry. [0003] H9 subtype avian influenza is a low-pathogenic disease, but mixed infection with other pathogens, especially Newcastle disease and E. hazards. [000...

Claims

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Application Information

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IPC IPC(8): C12N7/00A61K39/235A61K39/17A61K39/145A61K39/12A61K39/39A61P31/20A61P31/14A61P31/16C12R1/93
CPCA61K39/12A61K39/39A61K2039/5252A61K2039/541A61K2039/70A61P31/14A61P31/16A61P31/20C12N7/00C12N2710/10221C12N2710/10234C12N2720/10034C12N2760/16134C12N2760/18134A61K2300/00
Inventor 章振华
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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