Isoprene synthase and coding gene thereof, expression vector, engineering bacteria and method for producing isoprene and application

A technology of isoprene synthase and gene, which is applied in the field of genetic engineering, can solve the problems of low affinity of substrate DMAPP, blockage of metabolic flow, and low activity of isoprene synthase, so as to achieve good application prospects and alleviate obstacles. The effect of stagnant flux
CN109097378AActive Publication Date: 2018-12-28QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
Publication Date
2018-12-28

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Abstract

The invention discloses an isoprene synthase and a coding gene thereof, an expression vector, engineering bacteria and a method for producing isoprene and application, and belongs to the technical field of genetic engineering. The nucleotide sequence of the isoprene synthase gene IspSib is as shown in SEQ ID NO 2, and the amino acid sequence of the isoprene synthase is as shown in SEQ ID NO. 1. The invention also provides a prokaryotic expression vector containing the isoprene synthase gene and co-transformation of the prokaryotic expression vector and a downstream expression vector of mevalonate pathway to obtain an isoprene-producing fungus The present invention also provides a method for fermentative production of isoprene and the above-mentioned isoprene synthase gene, isoprene synthase, enzyme having isoprene synthase activity, prokaryotic expression vector thereof and The application of engineering bacteria in the production of isoprene. The isoprene synthase enzyme activity provided by the present invention is high and has high affinity for the substrate DMAPP.
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Description

technical field

[0001] The invention relates to an isoprene synthase, its coding gene, an expression vector, an engineering bacterium, a method and application for producing isoprene, and belongs to the technical field of genetic engineering. Background technique

[0002] Isoprene is an important chemical platform compound, 95% of which is used in synthetic rubber; it is also the second monomer of butyl rubber. In addition, isoprene is also widely used in the fields of pesticides, medicines, spices and adhesives. At present, the source of isoprene is mainly through petroleum-based raw material isopentane, isopentene dehydrogenation method, chemical synthesis method (including isobutylene-formaldehyde method, acetylene-acetone method, propylene dimerization method) and cracking C5 fraction extraction Distillation. However, with the increasing depletion of fossil resources, the source of raw materials is an important bottleneck issue for the preparation of isoprene from petr...

Claims

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