3 results about "Isoprene synthase" patented technology

The present invention relates to a transformant having isoprene-producing ability The transformant of the present invention has superior isoprene-producing ability by introduction, into methanotrophic bacteria, a nucleotide sequence encoding exogenous isoprene synthase, a nucleotide sequence encoding exogenous isopentenyl diphosphate isomerase, and a nucleotide sequence encoding exogenous 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, and thus can be effectively used for isoprene biosynthesis.

This invention discloses a method for constructing an enzyme cascade reactor using phosphorylated SBA-15 as a carrier to catalyze the synthesis of isoprene from mevalonic acid, belonging to the field of biocatalysis and synthetic biology. This method constructs a co-immobilized phosphatase cascade reactor consisting of mevalonate kinase (MVK), phosphate mevalonate kinase (PMK), mevalonate diphosphate decarboxylase (MVD), isopentenyl pyrophosphate isomerase (IDI), and isoprene synthase (ISPS), achieving highly efficient cascade catalysis from mevalonic acid (MVA) to isoprene. By immobilizing the five enzymes on a phosphorylated mesoporous SBA-15 carrier through hydrogen bonding, the stability, reusability, and catalytic efficiency of the enzymes are significantly improved, solving the problems of easy inactivation and difficulty in recovery of free enzymes. Simultaneously, optimized reaction conditions enable efficient, low-cost, and sustainable preparation of isoprene, suitable for the industrial production of bio-based isoprene.

The invention discloses a recombinant saccharomyces cerevisiae engineering bacterium which carries a gene expression cassette containing a hydroxymethyl glutaryl-coenzyme A reductase coding gene and an isoprene synthetase coding gene, and discloses the recombinant saccharomyces cerevisiae engineering bacterium which is constructed by adopting the engineering bacterium and is used for producing dammarendiol. The invention discloses a dammarendiol gene carrier which carries a gene expression cassette containing a dammarendiol synthetase gene, and further discloses a method for producing dammarendiol. According to the saccharomyces cerevisiae engineering strain constructed through synthetic biology and genetic engineering technical means, the dammarendiol synthetase can be efficiently recombined and expressed, then a precursor, namely the dammarendiol, of ginsenoside protopanoxadiol is synthesized, and a foundation is laid for finally achieving ginsenoside biosynthesis and large-scale industrial production.