44 results about "Isoprene synthase" patented technology

To provide a series of techniques capable of producing isoprene from syngas or the like. Provided is a recombinant cell prepared by introducing a nucleic acid encoding isoprene synthase into a host cell having an isopentenyl diphosphate synthesis ability by a non-mevalonate pathway, wherein the nucleic acid is expressed in the host cell, and the recombinant cell is capable of producing isoprene from at least one C1 compound selected from the group consisting of carbon monoxide, carbon dioxide, formic acid, and methanol. As the host cell, a Clostridium bacterium or a Moorella bacterium is exemplified. Also provided is a method for producing isoprene using the recombinant cell.

Provided are recombinant cells that are anaerobic archaea, wherein: the recombinant cells have a gene that encodes isoprene synthase, a gene that encodes monoterpene synthase, a gene that encodes sesquiterpene synthase, a gene that encodes diterpene synthase, a gene that encodes squalene synthase, or a gene that encodes phytoene synthase as a first foreign gene; and the recombinant cells are capable of expressing the first foreign gene and producing isoprene or terpenes having 10, 15, 20, 30, or 40 carbon atoms.

The present invention provides methods for establishing superior isoprene monomer production systems. Specifically, the present invention provides polynucleotides and analogs thereof of the following (a), (b) or (c): (a) polynucleotides comprising (i) nucleosides represented by SEQ ID NO:1 acid sequence, or (ii) a nucleotide sequence consisting of the 133rd to 1785th nucleotide residues in the nucleotide sequence represented by SEQ ID NO:1; (b) a polynucleotide comprising A nucleotide sequence having 90% or more identity to the nucleotide sequence of (i) or (ii) above, and the polynucleotide encodes a protein having isoprene synthase activity; or (c) A polynucleotide that hybridizes under stringent conditions to a polynucleotide consisting of a nucleotide sequence complementary to the nucleotide sequence of (i) or (ii) above, and the polynucleotide encodes a polynucleotide having isoprene synthesis Enzymatically active protein.

The invention relates to an isoprene synthetase gene and applications of the isoprene synthetase gene, for solving the technical problem that the efficiency for synthesizing isoprene by adopting engineering bacteria is not high. The invention provides the isoprene synthetase gene, protein expressed by the isoprene synthetase gene, a prokaryotic expression vector containing the isoprene synthetase gene, engineering bacteria, as well as preparation method and applications of the engineering bacteria capable of producing isoprene. The isoprene synthetase gene can be widely applied to the preparation field of isoprene.