44 results about "Isoprene synthase" patented technology

To provide a series of techniques capable of producing isoprene from syngas or the like. Provided is a recombinant cell prepared by introducing a nucleic acid encoding isoprene synthase into a host cell having an isopentenyl diphosphate synthesis ability by a non-mevalonate pathway, wherein the nucleic acid is expressed in the host cell, and the recombinant cell is capable of producing isoprene from at least one C1 compound selected from the group consisting of carbon monoxide, carbon dioxide, formic acid, and methanol. As the host cell, a Clostridium bacterium or a Moorella bacterium is exemplified. Also provided is a method for producing isoprene using the recombinant cell.

The present invention provides methods and compositions of polypeptides having isoprene synthase activity with improved performance characteristics. In particular, the present invention provides legume isoprene synthases for increased isoprene production in recombinant host cells.

Provided are recombinant cells that are anaerobic archaea, wherein: the recombinant cells have a gene that encodes isoprene synthase, a gene that encodes monoterpene synthase, a gene that encodes sesquiterpene synthase, a gene that encodes diterpene synthase, a gene that encodes squalene synthase, or a gene that encodes phytoene synthase as a first foreign gene; and the recombinant cells are capable of expressing the first foreign gene and producing isoprene or terpenes having 10, 15, 20, 30, or 40 carbon atoms.

The present invention provides means useful for establishing an excellent isoprene monomer production system. Specifically, the present invention provides a polynucleotide of the following (a), (b), or (c):(a) a polynucleotide comprising (i) the nucleotide sequence represented by SEQ ID NO:1, or (ii) the nucleotide sequence consisting of the nucleotide residues at positions 133 to 1785 in the nucleotide sequence represented by SEQ ID NO:1;(b) a polynucleotide that comprises a nucleotide sequence having 90% or more identity to the nucleotide sequence of (i) or (ii) above, and encodes a protein having an isoprene synthase activity; or(c) a polynucleotide that hybridizes under a stringent condition with a polynucleotide consisting of a nucleotide sequence complementary to the nucleotide sequence of (i) or (ii) above, and encodes a protein having an isoprene synthase activity; and the like.

The invention provides for compositions and methods for producing isoprene by using recombinantly engineered cells that utilize a system of dual IspG enzymes in addition to isoprene synthase.

The present invention relates to a recombinant methanotroph having an ability to produce isoprene and a method for producing isoprene using the same, and more particularly to a recombinant methanotroph having an ability to produce isoprene wherein a gene encoding an isoprene synthase having a homology of at least 70% to the amino acid sequence of Ipomoea batatas isoprene synthase is introduced into the recombinant methanotroph, and a method for producing isoprene using the recombinant methanotroph. The use of a recombinant methanotroph according to the present invention enables isoprene to be produced in high yield by using methane gas or methanol which is obtained from waste such as natural gas, biomass, municipal waste or the like as a carbon source.

The present invention provides methods for establishing superior isoprene monomer production systems. Specifically, the present invention provides polynucleotides and analogs thereof of the following (a), (b) or (c): (a) polynucleotides comprising (i) nucleosides represented by SEQ ID NO:1 acid sequence, or (ii) a nucleotide sequence consisting of the 133rd to 1785th nucleotide residues in the nucleotide sequence represented by SEQ ID NO:1; (b) a polynucleotide comprising A nucleotide sequence having 90% or more identity to the nucleotide sequence of (i) or (ii) above, and the polynucleotide encodes a protein having isoprene synthase activity; or (c) A polynucleotide that hybridizes under stringent conditions to a polynucleotide consisting of a nucleotide sequence complementary to the nucleotide sequence of (i) or (ii) above, and the polynucleotide encodes a polynucleotide having isoprene synthesis Enzymatically active protein.

Provided are a novel isoprene synthase derived from sweet potato and a method of preparing isoprene using the same, and more specifically, a novel isoprene synthase derived from sweet potato, a gene encoding the isoprene synthase, a host cell transformed with the gene, and a method of preparing isoprene using the same. The isoprene synthase of the present invention may have higher isoprene productivity as compared to isoprene synthases known in the related art to thereby be effectively used in isoprene biosynthesis and preparation of an isoprene polymer using the same.

The invention relates to an isoprene synthetase gene and applications of the isoprene synthetase gene, for solving the technical problem that the efficiency for synthesizing isoprene by adopting engineering bacteria is not high. The invention provides the isoprene synthetase gene, protein expressed by the isoprene synthetase gene, a prokaryotic expression vector containing the isoprene synthetase gene, engineering bacteria, as well as preparation method and applications of the engineering bacteria capable of producing isoprene. The isoprene synthetase gene can be widely applied to the preparation field of isoprene.