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In enzymology, an isoprene synthase (EC 4.2.3.27) is an enzyme that catalyzes the chemical reaction dimethylallyl pyrophosphate ⇌ isoprene + diphosphate Hence, this enzyme has one substrate, dimethylallyl pyrophosphate, and two products, isoprene and pyrophosphate. This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on phosphates. The systematic name of this enzyme class is dimethylallyl-pyrophosphate pyrophosphate-lyase (isoprene-forming).
Method for the synthesis of isoprene from mevalonate catalyzed by a phosphokinase cascade reactor
This invention discloses a method for constructing an enzyme cascade reactor using phosphorylated SBA-15 as a carrier to catalyze the synthesis of isoprene from mevalonic acid, belonging to the field of biocatalysis and synthetic biology. This method constructs a co-immobilized phosphatase cascade reactor consisting of mevalonate kinase (MVK), phosphate mevalonate kinase (PMK), mevalonate diphosphate decarboxylase (MVD), isopentenyl pyrophosphate isomerase (IDI), and isoprene synthase (ISPS), achieving highly efficient cascade catalysis from mevalonic acid (MVA) to isoprene. By immobilizing the five enzymes on a phosphorylated mesoporous SBA-15 carrier through hydrogen bonding, the stability, reusability, and catalytic efficiency of the enzymes are significantly improved, solving the problems of easy inactivation and difficulty in recovery of free enzymes. Simultaneously, optimized reaction conditions enable efficient, low-cost, and sustainable preparation of isoprene, suitable for the industrial production of bio-based isoprene.
Owner:QINGDAO UNIV OF SCI & TECH