43 results about "Isoprene synthase" patented technology

The invention features methods for producing isoprene from cultured cells having increased expression levels and/or activity levels of a mevalonate kinase polypeptide and an isoprene synthase polypeptide. The invention also provides methods for producing isoprene from cultured cells having reduced accumulation of intermediates (such as mevalonate, isopentenyl diphosphate, 3,3-dimethylallyl diphosphate, geranyl diphosphate, or farnesyl diphosphate) in the biosynthesis of isoprene or isoprenoids that may otherwise cause undesirable amounts of growth inhibition, toxicity, or cell death. The resulting isoprene compositions may have increased yields and/or purity of isoprene.

The invention provides for compositions and methods for producing isoprene using isoprene synthase variants with improved properties.

The invention provides for compositions and methods for producing isoprene by using recombinantly engineered cells that utilize a system of dual IspG enzymes in addition to isoprene synthase.

The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved specific productivity. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in host cells.

The present invention provides means useful for establishing an excellent isoprene monomer production system. Specifically, the present invention provides a polynucleotide of the following (a), (b), or (c):

• (a) a polynucleotide comprising (i) the nucleotide sequence represented by SEQ ID NO:1, or (ii) the nucleotide sequence consisting of the nucleotide residues at positions 133 to 1785 in the nucleotide sequence represented by SEQ ID NO:1;
• (b) a polynucleotide that comprises a nucleotide sequence having 90% or more identity to the nucleotide sequence of (i) or (ii) above, and encodes a protein having an isoprene synthase activity; or
• (c) a polynucleotide that hybridizes under a stringent condition with a polynucleotide consisting of a nucleotide sequence complementary to the nucleotide sequence of (i) or (ii) above, and encodes a protein having an isoprene synthase activity; and the like.

The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.

This document describes biochemical pathways for producing butadiene by forming two vinyl groups in a butadiene synthesis substrate. These pathways described herein rely on enzymes such as mevalonate diphosphate decarboxylase, isoprene synthase, and dehydratases for the final enzymatic step.

Provided are a novel isoprene synthase derived from sweet potato and a method of preparing isoprene using the same, and more specifically, a novel isoprene synthase derived from sweet potato, a gene encoding the isoprene synthase, a host cell transformed with the gene, and a method of preparing isoprene using the same. The isoprene synthase of the present invention may have higher isoprene productivity as compared to isoprene synthases known in the related art to thereby be effectively used in isoprene biosynthesis and preparation of an isoprene polymer using the same.

The invention discloses an isoprene synthase and a coding gene thereof, an expression vector, engineering bacteria and a method for producing isoprene and application, and belongs to the technical field of genetic engineering. The nucleotide sequence of the isoprene synthase gene IspSib is as shown in SEQ ID NO 2, and the amino acid sequence of the isoprene synthase is as shown in SEQ ID NO. 1. The invention also provides a prokaryotic expression vector containing the isoprene synthase gene and co-transformation of the prokaryotic expression vector and a downstream expression vector of mevalonate pathway to obtain an isoprene-producing fungus The present invention also provides a method for fermentative production of isoprene and the above-mentioned isoprene synthase gene, isoprene synthase, enzyme having isoprene synthase activity, prokaryotic expression vector thereof and The application of engineering bacteria in the production of isoprene. The isoprene synthase enzyme activity provided by the present invention is high and has high affinity for the substrate DMAPP.

The invention provides a method for improving an isoprene synthesis capability of saccharomyces cerevisiae. An IspS (isoprene synthase) gene sequence SEQ ID NO: 1 from a white poplar is cloned to a plasmid through enzyme digestion and connection and is converted into a saccharomyces cerevisiae parent body, so that isoprene is synthesized in the saccharomyces cerevisiae; a transcriptional level of IspS gene is enhanced through GAL4 over-expression; the catalytic activity of IspS is improved through orthogenesis. The saccharomyces cerevisiae and the isoprene synthase are combined together, so that the yield of isoprene is improved to be 8.2 times as much as the original yield.

The invention provides a useful means for establishing an excellent isoprene monomer production system. Specifically, the invention provides polynucleotides and the like of (a), (b), or (c) below: (a) polynucleotide containing (i) a base sequence represented by SEQ ID NO: 1 or (ii) a base sequence comprising nucleotide residue numbers 133-1785 in the base sequence represented by SEQ ID NO: 1; (b) polynucleotide containing a base sequence having 90% or greater identity with the base sequences (i) or (ii) above, and encoding a protein having isoprene synthase activity; or (c) a polynucleotide that hybridizes with a polynucleotide comprising a base sequence complementary to the base sequences (i) or (ii) above, under stringent conditions, and encodes a protein having isoprene synthase activity.

The invention relates to an isoprene genetic engineering producing strain and anapplication thereof. By introducing an exogenous mevalonic acid path in a hot cell and overexpressing isoprene synthase, more preferably enhancing a methyl erythritol phosphate in the host cell, isoprene is efficiently produced by applying a prokaryotic cell.

The present disclosure relates to a novel method, expression vectors, and host cells for producing abienol by converting geranylgeranyl diphosphate (GGPP) to abienol in the presence of a combination of a class II diterpene synthase and a bifunctional class I/II abienol synthase.

Modified isoprene synthases that have one or more mutations of a given amino acid residue(s) in any amino acid sequence of

• • (a) the amino acid sequence of SEQ ID NO:4,
  • (b) an amino acid sequence having one or several amino acid substitutions, deletions, insertions or additions in the amino acid sequence of SEQ ID NO:4, or
  • (c) an amino acid sequence having 90% or more identity to the amino acid sequence of SEQ ID NO:4,

and has an isoprene synthetic activity are useful for preparing isoprene monomer in improved yields.

The invention discloses a method for producing isoprene by in vitro enzymatic reaction and an application of isoprene and belongs to the technical field of biological engineering. The method disclosed by the invention comprises the following steps of respectively introducing an obtained mevalonate kinase gene, a phosphomevalonate kinase gene, a mevalonate-pyrophosphate decarboxylase gene, an isopentenyl pyrophosphate isomerase gene and an isoprene synthase into host bacteria, carrying out fermental cultivation to obtain five recombinant bacteria, respectively carrying out ultrasonic disruption on each thallus to obtain a crude enzyme solution, mixing five crude enzyme solutions to obtain a reaction enzyme solution and adding a reaction substrate solution into the reaction enzyme solution to in vitro synthesize isoprene. By the method disclosed by the invention, the in-vitro synthesis of isoprene from mevalonic acid by virtue of enzymatic reaction is achieved and the synthesis of isoprene by precisely in vitro controlling the content of metabolic enzyme is realized.