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Extraction and purification method of triterpene acid in seabuckthorns, triterpene acid, triterpenoid saponin and application of triterpene acid and triterpenoid saponin

A purification method and technology of triterpenoid saponins are applied in the directions of chemical instruments and methods, applications, food ingredients containing natural extracts, etc., and can solve the problems of poor separation effect and low purity of ursolic acid and oleanolic acid, and achieve The effect of enhancing immunity, high purity and good solubility

Inactive Publication Date: 2019-01-01
QINGHAI TSINGHUA BIOTRY BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The first purpose of the present invention is to provide a method for extracting and purifying triterpene acids in Seabuckthorn, so as to alleviate the poor separation effect and low purity of ursolic acid and oleanolic acid in the extraction and purification of Seabuckthorn existing in the prior art. question

Method used

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  • Extraction and purification method of triterpene acid in seabuckthorns, triterpene acid, triterpenoid saponin and application of triterpene acid and triterpenoid saponin
  • Extraction and purification method of triterpene acid in seabuckthorns, triterpene acid, triterpenoid saponin and application of triterpene acid and triterpenoid saponin
  • Extraction and purification method of triterpene acid in seabuckthorns, triterpene acid, triterpenoid saponin and application of triterpene acid and triterpenoid saponin

Examples

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Effect test

Embodiment 1

[0105] This embodiment provides a method for extracting and purifying triterpene acids in Seabuckthorn, comprising the following steps:

[0106] (a) Dry seabuckthorn pericarp at 75°C for 30 minutes, crush it to a particle size of 90 mesh, use 75% ethanol as the extractant, the mass ratio of seabuckthorn peel and 75% ethanol is 1:10, and ultrasonically extract for 30 minutes at room temperature to obtain the extract ;

[0107] (b) process extract with 1.5mol / L sodium hydroxide solution earlier, adjust pH to 11, then process extract with 1.5mol / L hydrochloric acid solution, adjust pH to 3, obtain ursolic acid and oleanolic acid solid, Rinse ursolic acid and oleanolic acid solids with water for 3 times, dissolve ursolic acid and oleanolic acid solids in 75% absolute ethanol to obtain a mixed solution;

[0108] (c) The macroporous resin is packed into a column, and the mass ratio of D101 macroporous resin and X-5 macroporous resin in the macroporous resin is 3:13, and ursolic aci...

Embodiment 2

[0111] This embodiment provides a method for extracting and purifying triterpene acids in Seabuckthorn, comprising the following steps:

[0112] (a) Dry seabuckthorn pericarp at 75°C for 30 minutes, crush it to a particle size of 90 mesh, use 75% ethanol as the extractant, the mass ratio of seabuckthorn peel and 75% ethanol is 1:10, and ultrasonically extract for 30 minutes at room temperature to obtain the extract ;

[0113] (b) process extract with 1.5mol / L sodium hydroxide solution earlier, adjust pH to 11, then process extract with 1.5mol / L hydrochloric acid solution, adjust pH to 3, obtain ursolic acid and oleanolic acid solid, Rinse ursolic acid and oleanolic acid solids with water for 3 times, dissolve ursolic acid and oleanolic acid solids in 75% absolute ethanol to obtain a mixed solution;

[0114] (c) The macroporous resin is packed into a column, the mass ratio of D101 macroporous resin and X-5 macroporous resin in the macroporous resin is 3:10, and ursolic acid an...

Embodiment 3

[0117] This embodiment provides a method for extracting and purifying triterpene acids in Seabuckthorn, comprising the following steps:

[0118] (a) Dry seabuckthorn pericarp at 75°C for 30 minutes, crush it to a particle size of 90 mesh, use 75% ethanol as the extractant, the mass ratio of seabuckthorn peel and 75% ethanol is 1:10, and ultrasonically extract for 30 minutes at room temperature to obtain the extract ;

[0119] (b) process extract with 1.5mol / L sodium hydroxide solution earlier, adjust pH to 11, then process extract with 1.5mol / L hydrochloric acid solution, adjust pH to 3, obtain ursolic acid and oleanolic acid solid, Rinse ursolic acid and oleanolic acid solids with water for 3 times, dissolve ursolic acid and oleanolic acid solids in 75% absolute ethanol to obtain a mixed solution;

[0120] (c) The macroporous resin is packed into a column, the mass ratio of D101 macroporous resin and X-5 macroporous resin in the macroporous resin is 1:15, and ursolic acid an...

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Abstract

The invention provides an extraction and purification method of triterpene acid in seabuckthorns, triterpene acid, triterpenoid saponin and application of the triterpene acid and the triterpenoid saponin and relates to the technical field of extraction and purification. The extraction and purification method of the triterpene acid in the seabuckthorns comprises the following steps of (a) extracting ursolic acid, oleanolic acid and derivatives thereof in seabuckthorn peels with an organic solvent to obtain an extract; (b) treating the extract with an alkali solution firstly, then treating the extract with an acid solution to obtain ursolic acid solid and oleanolic acid solid, and dissolving the ursolic acid solid and the oleanolic acid solid into the organic solvent to obtain a mixed solution; (c) adsorbing the ursolic acid and the oleanolic acid in the mixed solution with macroporous resin, and performing elution and concentration with an eluent after adsorption is finished to obtain acrude product; and (d) adopting HPLC or HSCCC to perform separation on the ursolic acid and the oleanolic acid in the crude product. In the extraction and purification method provided by the invention, the separation effect of the ursolic acid and the oleanolic acid is good, the purity of the ursolic acid reaches up to 97.6 percent or above, and the purity of the oleanolic acid reaches up to 98.2percent or above.

Description

technical field [0001] The invention relates to the technical field of extraction and purification, in particular to a method for extracting and purifying triterpene acids from Hippophae rhamnoides, triterpene acids and triterpene saponins and applications thereof. Background technique [0002] As a local characteristic crop variety, seabuckthorn can prevent and fix sand and bring good economic and sociological value to the local social and economic development. The triterpene acids in seabuckthorn mostly exist in plants in the free state or in the form of glycosides or esters, and are almost insoluble or hardly soluble in water. The triterpene acids in seabuckthorn are mainly ursolic acid and oleanolic acid, which have the functions of protecting the liver, lowering blood sugar and anti-oxidation of the skin. However, the current extraction and purification of ursolic acid and oleanolic acid in seabuckthorn has poor separation effect and low purity. [0003] In view of th...

Claims

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Application Information

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IPC IPC(8): C07J63/00C07H1/00C07H15/256A61P1/16A61P3/10A61P3/06A61P3/04A61P9/10A23L33/11
CPCA23L33/11A23V2002/00A61P1/16A61P3/04A61P3/06A61P3/10A61P9/10C07H1/00C07H15/256C07J63/008A23V2200/302A23V2200/328A23V2200/332A23V2200/326A23V2250/21
Inventor 龚晨于雪李婷吴鹏
Owner QINGHAI TSINGHUA BIOTRY BIO TECH
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