Spore-producing bacillus amyloliquefaciens for inhibiting plant pathogenic fungi and application of spore-producing bacillus amyloliquefaciens

A technology for plant pathogenic fungi and amylolytic spores, which is applied in the fields of microbiology and biology to achieve the effects of high inhibition rate, high antibacterial spectrum, and broad antibacterial spectrum.

Pending Publication Date: 2019-01-04
SICHUAN UNIV +1
View PDF7 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few biocontrol agents that can efficiently antagonize plant pathogenic fungi. Therefore, a microbial strain that can efficiently antagonize plant pathogenic fung

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Spore-producing bacillus amyloliquefaciens for inhibiting plant pathogenic fungi and application of spore-producing bacillus amyloliquefaciens
  • Spore-producing bacillus amyloliquefaciens for inhibiting plant pathogenic fungi and application of spore-producing bacillus amyloliquefaciens
  • Spore-producing bacillus amyloliquefaciens for inhibiting plant pathogenic fungi and application of spore-producing bacillus amyloliquefaciens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Evaluation of the production potential of Bacillus amyloliquefaciens 1841

[0048] After sterilizing 50 ml of NBY medium (pH 7.0) at 120°C and 0.8 atmospheres for 30 min, place it in a 750 ml sterile vial and cool to 20°C. After inoculating Bacillus amyloliquefaciens 1841 in the culture medium at 30°C and 250 rpm / min for 16 h, 5-10 ml of culture was obtained. Microscopy to ensure that there is no contamination, the concentration of the culture product is checked by the microscope, and the number of cells should reach 10 9 cfu / ml.

[0049] After the above-mentioned cultured Bacillus amyloliquefaciens 1841 and the control strain Bacillus amyloliquefaciens CGMCC No. 4777, which has the best antibacterial effect, were cultured on YM medium for 72 hours, the productivity (titer) of the two strains was observed. , The results are shown in Table 1.

[0050] Table 1 Comparison of productivity (titer) between the strain of the invention and the control strain

[0051]

[00...

Embodiment 2

[0053] Example 2 Evaluation of antifungal activity of Bacillus amyloliquefaciens 1841

[0054] The punching method was used to detect Bacillus amyloliquefaciens 1841 and the control strain Bacillus amyloliquefaciens CGMCC NO.4777, against Sclerotinia sclerotiorum, Alternaria tenuis, Fusarium oxysporum, Pyricularia oryzae, Gray leaf spot, Botrytis cinerea The bacteriostatic activity and the bacteriostatic range (mm) are shown in Table 2.

[0055] Table 2 Comparison of antibacterial activity of the present strain and the control strain

[0056]

[0057] Description: 1. Sclerotinia sclerotiorum, Alternaria tenuis, Fusarium oxysporum, Pyricularia oryzae, and Gray leaf spot are the inhibitory range of fungal growth when using a 1:500 diluted bacterial culture solution.

[0058] 2. Botrytis cinerea is the antibacterial growth range when using undiluted bacterial culture solution.

[0059] It can be seen from Table 2 that the ability of Bacillus amyloliquefaciens 1841 of the present invention...

Embodiment 3

[0060] Example 3 Evaluation of antifungal activity of Bacillus amyloliquefaciens 1841 culture solution in different dilutions

[0061] The punching method was used to detect different dilution concentrations of Bacillus amyloliquefaciens 1841 and the control strain Bacillus amyloliquefaciens CGMCC NO.4777 culture solution, co-inoculated with Sclerotinia sclerotiorum, Alternaria tenuis, Fusarium oxysporum, and corn ash The antifungal activity of plaque fungus and the growth of fungi (mm) are shown in Table 3.

[0062] Table 3 Comparison of antifungal activity of the present strain and the control strain under different dilution concentrations of culture solution co-inoculation

[0063]

[0064] It can be seen from Table 3 that the ability of Bacillus amyloliquefaciens 1841 of the present invention to inhibit the growth of Sclerotinia sclerotiorum, Alternaria tenuis, Fusarium oxysporum, and Pyricularia oryzae is better than the control bacterium CGMCC No. 4777, while inhibiting corn T...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to view more

Abstract

The invention relates to microbiology and biotechnology, and particularly discloses spore-producing bacillus amyloliquefaciens for inhibiting plant pathogenic fungi and an application of spore-producing bacillus amyloliquefaciens in prevention and treatment of gray mold. The Bacillus amyloliquefaciens 1841 is preserved in the China General Microbiological Culture Collection Center with preservation number of CGMCC No. 15465 on March 20, 2018. The strain is introduced from Russia and has broad antibacterial spectrum and high inhibition rate. Fermentation liquor and bacteriostatic substances ofthe strain and a preparation prepared from the fermentation liquor are used for preventing and treating gray mold caused by botrytis cinerea and other fungal diseases, and the bacillus amyloliquefaciens has good effect, can be industrially produced, and is simple to use, non-toxic, pollution-free, green, environmentally friendly and the like, and a preparation process is simple.

Description

Technical field [0001] The present invention relates to microbiology and biotechnology, in particular to a strain of Bacillus amyloliquefaciens 1841 for inhibiting the growth of plant pathogenic fungi and its application. Background technique [0002] The preservation of crops including vegetables, fruits and cereals is an important issue in agriculture. The infection of plants by plant pathogenic fungi can cause serious economic and social problems. Pathogenic fungi can affect plants at different stages of plant vegetative growth, and can also cause huge losses during vegetable storage. Every year, 25-50% of crop storage in various countries in the world are infected by fungi, which can cause up to 30% of storage losses. Diseases caused by plant pathogenic fungi can also reduce the quality of food and seed materials. Infected plants also become a source of transmission of various pathogens during the growing season. Chemical fungicides are often used to protect plants agains...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 孙群刘健熊仁科左建英鲁道夫·阿泽贝卡亚娜塔利亚·库泽那塔索娃
Owner SICHUAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap