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A mold and yeast count test piece, preparation method and application thereof

A test piece, yeast technology, applied in mold and yeast count test piece, the application field of detection of mold and yeast count in food, can solve the problem of small colonies, unable to count, unable to indicate mold and yeast, etc.

Active Publication Date: 2021-10-22
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The outstanding problems are concentrated in three aspects: the spread and growth of mold hyphae affect the counting or even make it impossible to count; Color development cannot indicate all molds and yeasts, it needs to be perfected to prevent missed detection

Method used

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  • A mold and yeast count test piece, preparation method and application thereof
  • A mold and yeast count test piece, preparation method and application thereof
  • A mold and yeast count test piece, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Preparation of mould and yeast enumeration medium

[0032] The original weight components of the mold and yeast counting color detection medium: peptone 15g, glucose 15g, ethanol 10mL, copper sulfate 0.2g, calcium chloride 0.2g, zinc chloride 0.15g, magnesium sulfate 4g, potassium dihydrogen phosphate 0.7 g, ferric sulfate 0.71 g, 5-bromo-4-chloro-3-indole phosphate (BCIP) 0.2 g, p-nitrophenyl palmitate 0.2 g, chloramphenicol 0.1 g, and distilled water 1000 mL.

[0033] The preparation method of mold and yeast counting detection medium is: 15g peptone, 15g glucose, 8mL ethanol, 0.2g copper sulfate, 0.2g calcium chloride, 0.15g zinc chloride, 4g magnesium sulfate, 0.7g potassium dihydrogen phosphate, 0.71g of ferric sulfate was added to 900mL of distilled water, heated and stirred to dissolve, autoclaved at 121°C for 15min, and cooled to room temperature; 0.2g of p-nitrophenyl palmitate was dissolved in 2mL of ethanol, and 0.2g of BCIP was dissolved in 100mL of...

Embodiment 2

[0035] Example 2 Preparation of mold and yeast count test pieces

[0036] 1) The test piece consists of three layers, namely the upper layer of PET silicone film, the middle layer of polypropylene plastic plate with hollow area, and the bottom plate of white checkered copper plate paper. All three parts were cut into 10.5cm×9cm, and the hollow area in the middle (a circle with a diameter of 6cm) was bonded to the bottom plate to form a culture area. The bottom plate was printed with a light orange 1cm×1cm square for easy counting. The upper, middle and lower parts are bonded to each other with polyacrylic resin pressure-sensitive adhesive, and the bonding width is 5mm.

[0037] 2) The compounded cold water soluble gel powder is evenly coated on the inner side of the upper film of the test piece with a thickness of 0.15mm, and the mass of the cold water gel of each test piece is 0.2g.

[0038] 3) With a density of 60g / m 2 , a circular non-woven fabric with a diameter of 6 cm ...

Embodiment 3

[0042] Embodiment 3 utilizes test piece to measure the method for mould and yeast count in food

[0043] 1) Weigh 25g of the food sample to be tested by aseptic operation (the sample to be tested can be solid food such as rice and flour products, biscuits, cakes, etc.) or the food sample to be tested (the sample to be tested can be liquid food such as beverages), add 25mL to 225mL to dilute In a sterile homogenizing bag of the liquid, beat with a homogenizer for 1-2 min to prepare a 1:10 sample homogenate solution. Do a 10-fold serial gradient dilution of the above sample homogenate. According to the degree of contamination of the sample, choose 2 to 3 dilutions, and make two parallel samples for each dilution.

[0044] 2) Take 1 mL of the diluent of the food sample to be tested, lift off the upper film of the test piece, add it to the culture medium area, distribute the sample liquid evenly in the entire culture area, slowly drop the upper film, avoid squeezing your fingers, ...

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Abstract

The invention relates to a mold and yeast counting test piece, a preparation method and its application in detecting the counting of molds and yeasts in food, belonging to the technical field of food detection. The test piece is composed of a transparent plastic upper film with an adhesive lower surface, a plastic middle layer with a hollowed out area, and a bottom plate printed with squares from top to bottom; the temperature range of the above materials is 130 ° C ~ 150 ° C The viscous lower surface of the transparent plastic upper film is coated with a compound cold water soluble gel, and the hollow area is filled with a sterilized non-woven fabric with the same shape as the hollow area and impregnated with the color development medium for counting mold and yeast. The present invention uses the test piece to measure the count of mold and yeast in food. The components of the culture medium can accelerate the proliferation of mold spores and yeast and the formation of colonies, and inhibit the spread and growth of mold hyphae, which solves the problem of mold mycelium proliferation and yeast growth. Technical difficulties due to slow growth resulting in colony coverage, poor enumeration accuracy and long time required.

Description

technical field [0001] The invention belongs to the technical field of food detection, and in particular relates to a mold and yeast counting test piece, a preparation method and its application in detecting mold and yeast counting in food. Background technique [0002] Mold and yeast count measurement indicators are important indicators to reflect the quality of food hygiene, and are used to determine the degree of food contamination. The determination of mold and yeast count is the detection index of beverages, nut products, rice and flour products, cakes and other foods in my country, and it is also an important monitoring item for food risk prediction and hazard assessment. At present, GB4789.15 "National Food Safety Standard for Food Microbiological Inspection Mold and Yeast Counting" adopts the mold and yeast plate counting method. This method requires the preparation of culture medium, sterilization, glassware cleaning, etc. The procedures are cumbersome and the workl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/06
CPCC12Q1/06
Inventor 陈萍刘桐任大勇徐琳琳董娜刘爽修琳毕云枫崔雯雯郑慧纹
Owner JILIN AGRICULTURAL UNIV
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