A kind of agaric fungus clean basidiospore collection method

Abstract

The invention discloses a method for collecting clean basidiospores of agaric fungi, which comprises: dipping a cotton ball into 70% ethanol to wipe the surface of the fruiting body, cutting off the stipe from the joint between the stipe and the cap, and placing the cap upside down on the superstructure. In the clean bench; fold the sulfuric acid paper in half twice and cut off the right angle, unfold the sulfuric acid paper and put it into the bottom of the petri dish, take two rubber bands that can be autoclaved on the petri dish, and place the cap on the rubber band , with gills or fungi facing down, cover the petri dish and let it stand for 4-24 hours; remove the fruiting body cap, remove the rubber band, and fold the sulfuric acid paper in half twice so that the side containing the spores is on the On the inside, spread the sulfuric acid paper into a funnel shape and place it above the mouth of the centrifuge tube, rinse the inside of the sulfuric acid paper evenly with sterile water to collect basidiospores. The invention can obtain clean basidiospores, has high spore recovery rate and simple operation.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a method for collecting clean basidiospores of agaric fungi. Background technique [0002] Basidiospores are important materials for the isolation and cultivation of agaric fungi, genetic improvement and ecological habit research, and their morphological characteristics are also an important basis for the identification of agaric fungi. The collection of pure basidiospores is extremely critical for the study of agaric fungi. At present, the collection methods of agaric fungus basidiospores mainly include bagging collection method, spore print collection method, ejection collection method and so on. In terms of collection methods of basidiospores, Li Rongtong mentioned in "Acta Edible Fungi Sinica" 2010 17(1): 44-47 "Collection and Germination Conditions of Sclerotia Pleurotus Basidiospores" that the use of gill-fold washing method to collect sclerotia Pleurotus s...

AI Technical Summary

Problems solved by technology

In terms of collection methods of basidiospores, Li Rongtong mentioned in "Acta Edible Fungi Sinica" 2010 17(1): 44-47 "Collection and Germination Conditions of Sclerotia Pleurotus Basidiospores" that the use of gill-fold washing method to collect sclerotia Pleurotus spores Spores, in this method, the gills are in direct contact with the spore collection liquid, which increases the probability of contamination. In addition, this method is not suitable for the collection of basidiospores of the genus Boletus agaricus containing bacteria pores

Chinese Patent Publication No. CN103688746A disclosed "A Method for Collecting and Cultivating Edible Mushroom Spores" on April 02, 2014. This method is a traditional spore printing method. Since the sporocarp is in contact with the collection paper during the spore collection process, it is difficult to ensure collection. The cleanliness of the spores, in addition the spores collected by the collection paper are difficult to transfer; Chinese Patent Publication No. CN105039178A disclosed on November 11, 2015 "a method for the germination of the basidiospores of Dictyostelium oberatus". The solid tissue adheres to the inner wall of the test tube, so it is difficult to ensure the cleanliness of the spores; in addition, due to the diameter of the test tube, most spores of the fruiting bodies cannot be collected; at the same time, it is difficult to transfer the spores collected in the test tube; Chinese Patent Publication No. CN 106754624A disclosed "a method for the germination of Ganoderma lucidum basidiospores" on May 31, 2017. The collection environment of this method is relatively closed, and the fruiting body is easy to rot, so it is not suitable for the collection of agaric fungus basidiospores; There are many physical contacts, and it is difficult to obtain clean basidiospores