Magnetic microsphere carrier used for adsorbing endotoxin and preparation method thereof

A magnetic microsphere and endotoxin technology, applied in the field of medicine, can solve the problems of human health threats, high treatment costs, and family burdens, and achieve the effects of no side effects and adverse reactions, low production costs, and low recurrence rate

Pending Publication Date: 2019-01-15
ZIBO KANGBEI MEDICAL DEVICES
View PDF7 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The high cost of sepsis treatment and poor prognosis not only poses a huge threat to human health, but also brings a heavy burden to the patient's family

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Magnetic microsphere carrier used for adsorbing endotoxin and preparation method thereof
  • Magnetic microsphere carrier used for adsorbing endotoxin and preparation method thereof
  • Magnetic microsphere carrier used for adsorbing endotoxin and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0021] The present invention also provides a method for preparing a magnetic microsphere carrier for absorbing endotoxin, comprising the steps of:

[0022] The magnetic material is uniformly mixed by ball milling, and then made into a spherical substance; wherein, the magnetic material includes iron oxide and silicon, and the mass ratio of iron oxide and silicon is (7-8):1;

[0023] Wrap the polymer layer on the surface of the spherical substance to obtain magnetic microspheres; the raw material components of the polymer layer are calculated in parts by weight, including: 10-12 parts by weight of PVP, 5-7 parts by weight of cellulose acetate, and 2-5 parts by weight of collodion 1-3 parts by weight of cellulose, 8-10 parts by weight of gelatin, 5-6 parts by weight of polystyrene and 7-9 parts by weight of albumin; the mass ratio of spherical substance and polymer layer is 1:(2-3 );

[0024] Put the magnetic microspheres in 0.1-0.2 mol / L antibody ligand aqueous solution, adjus...

Embodiment 1

[0027] This embodiment provides a magnetic microsphere carrier for adsorbing endotoxin. The particle size of the magnetic microsphere carrier is 10 nm to 100 μm. The magnetic microsphere carrier includes an inner core, an intermediate layer and an outer layer; wherein, the inner core has superparamagnetism, The raw material components include iron oxide and silicon; the middle layer is a polymer layer, and the raw material components include PVP, cellulose acetate, collodion, cellulose, gelatin, polystyrene and albumin; the outer layer is an antibody ligand-based layer, The antibody ligand is polymyxin B ligand.

Embodiment 2

[0029] This embodiment provides a method for preparing a magnetic microsphere carrier for adsorbing endotoxin, comprising steps:

[0030] The magnetic material is mixed evenly by ball milling, and then made into a spherical substance; wherein, the magnetic material includes iron oxide and silicon, and the mass ratio of iron oxide and silicon is 7.5:1;

[0031] Wrap the polymer layer on the surface of the spherical substance to obtain magnetic microspheres; the raw material components of the polymer layer include: 11 parts by weight of PVP, 6 parts by weight of cellulose acetate, 3 parts by weight of collodion, 2 parts by weight of cellulose parts by weight, 9 parts by weight of gelatin, 5.5 parts by weight of polystyrene and 8 parts by weight of albumin; the mass ratio of the spherical substance to the polymer layer is 1:2.5;

[0032] Put the magnetic microspheres in 0.15 mol / L antibody ligand aqueous solution, adjust the pH to 9.7, shake and react overnight at 53°C, filter af...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
Login to view more

Abstract

The invention relates to a magnetic microsphere carrier used for adsorbing endotoxin and a preparation method thereof. The magnetic microsphere carrier comprises a kernel, an interlayer and an outer layer, wherein the kernel is superparamagnetic, and is prepared from the raw material components of ferric oxide and silicon; the interlayer is a high-molecular layer which is prepared from the raw material components of PVP (Polyvinyl Pyrrolidone), cellulose acetate, collodion, cellulose, gelatin, polystyrene and albumin; the outer layer is an antibody ligand-carrying base layer, wherein an antibody ligand is a polymyxin B ligand. By adopting the magnetic microsphere carrier provided by the invention, specific toxin in a patient can be effectively adsorbed, and endotoxin is cleared away by using extracorporeal circulation; the magnetic microsphere carrier is released into human body blood through the extracorporeal circulation to finish the adsorption, and the magnetic adsorption is finished before the magnetic microsphere carrier enters the human body, so that the living quality of the patient is improved.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a magnetic microsphere carrier for absorbing endotoxin and a preparation method thereof. Background technique [0002] Sepsis refers to the systemic inflammatory response syndrome caused by infection factors, which can lead to organ dysfunction and / or circulatory disturbance in severe cases, and is a common complication of severe trauma, burns, shock, infection and major surgery; Sepsis is also common in patients with chronic diseases such as diabetic chronic obstructive bronchitis, leukemia, aplastic anemia, and patients with urinary calculi. According to the severity of sepsis, it can be divided into sepsis, severe sepsis and septic shock, and further development can lead to multiple organ dysfunction syndrome. Endotoxin (LPS) can directly or indirectly stimulate macrophages, neutrophils, and endothelial cells in the body to produce a series of inflammatory cytokines and vasoactive fac...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/26B01J20/28B01J20/30A61M1/36
CPCA61M1/36B01J20/02B01J20/06B01J20/22B01J20/26B01J20/28009B01J20/28019
Inventor 吴越
Owner ZIBO KANGBEI MEDICAL DEVICES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap