Sesquiterpene compound and preparation method and application thereof

A compound, sesquiterpene technology, applied in the field of pinane-type sesquiterpenoids and their separation and purification, can solve the problems of no literature reports

Active Publication Date: 2019-01-18
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] According to literature research, the two sesquiterpene compounds involved i

Method used

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  • Sesquiterpene compound and preparation method and application thereof
  • Sesquiterpene compound and preparation method and application thereof
  • Sesquiterpene compound and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The preparation method of compound 1 and 2 shown in embodiment 1. formula I

[0024] With Trichoderma virens gained according to above-mentioned literature or public circulation channel, get the Trichoderma virens (size 2.0 centimeters * 2.0 centimeters) that grows in the PDA plate culture medium and inoculate in the rice solid culture medium that has sterilized, Standing culture at room temperature for 30 days, the fermentation product was soaked and extracted 3 times with ethyl acetate, and the combined extracts were concentrated to obtain a fermented crude extract;

[0025] The formula of the rice solid medium is: every 100 milliliters of distilled water contains 70 grams of rice, 0.2 grams of corn steep liquor, and 0.3 grams of peptone;

[0026] The crude extract was subjected to vacuum silica gel column chromatography, and the petroleum ether-ethyl acetate with a gradient of 20:1 to 1:1 (v / v, the same below) and the gradient of 20:1 to 1:1 dichloromethane- Methano...

Embodiment 2

[0037] Example 2. Bacteriostatic activity

[0038] The antibacterial activity of compounds 1 and 2 shown in formula I was detected by the minimum inhibitory concentration method. The following bacterial strains were selected for antibacterial activity test: human pathogenic bacteria (1 strain): Escherichia coli (Escherichia coli); aquatic pathogenic bacteria (10 strains): Aeromonas hydrophilia (Aeromonas hydrophilia), catfish Edwardsiella (Edward megi), Edwardsiella tarda, Micrococcus luteus, Pseudomonasaeruginosa, Vibrio alginolyticus, V. anguillarum, Harvestella Vibrio harveyi, V.parahemolyticus, V.vulnificus; agricultural disease fungi (15 strains): tomato early blight (Alternaria solani), wheat root rot (Bipolarissorokiniana ), Ceratobasidium cornigerum, Coniothyrium diplodiella, Colletottichum gloeosporioides, Colletottichum gloeosporioides Penz, Fusarium graminearum, Pepper root rot (Fusarium solani), Fusarium oxysporum, Fusarium oxysporum. sp. cucumebrium Owen, Fusari...

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Abstract

The invention relates to the technical field of microbial medicines, in particular to a cadinane type sesquiterpenoid compound obtained from a fermentation product of Trichoderma virens, and a separation and purification method and application thereof. The molecular formulas of the sesquiterpene compound are C15H16O3 (compound 1) and C15H22O2 (compound 2), respectively, as shown in formulas 1 and2. The compounds 1 and 2 obtained by the invention have relatively good and broad-spectrum antibacterial activity and are expected to be further developed into a suppressing drug for treating infections caused by various bacteria or fungi. The formula is shown in the description.

Description

technical field [0001] The invention relates to the technical field of microbial medicine, in particular to a juniperane-type sesquiterpene compound obtained from a fermentation product of Trichodermavirens, a separation and purification method and application thereof. Background technique [0002] Microbial secondary metabolites have rich structural diversity and significant biological activity, and are an important source of drug lead compounds. Cindinane-type sesquiterpenoids are a class of biologically active compounds mainly produced by fungi. They are sesquiterpenes with a bicyclic structure and have significant antibacterial activity. [0003] According to literature research, the two sesquiterpene compounds involved in the present invention are new compounds, which have not been reported in the literature before. Contents of the invention [0004] The object of the present invention is to provide a method for obtaining juniperane-type sesquiterpenoids from the fer...

Claims

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Application Information

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IPC IPC(8): C07D307/92C07C61/35C12P7/40C12P17/04A01N43/12A01N37/08A01P1/00A01P3/00A61K31/343A61K31/19A61P31/04A61P31/10C12R1/885
CPCA61P31/04A61P31/10A01N37/08A01N43/12A61K31/19A61K31/343C12P7/40C12P17/04C07C61/35C07D307/92C07C2602/10C07B2200/07Y02A50/30
Inventor 王斌贵石小杉李晓明
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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