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Double-emitting fluorescent nanoparticle preparation method

A dual-emission fluorescence and nanoparticle technology, which is applied in the field of preparation of dual-emission fluorescence nanoparticles, achieves the effects of mild reaction conditions, good stability, and simple and easy-to-obtain raw materials

Active Publication Date: 2019-01-25
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, there is no report about chemically bonding Rhodamine B to PLGA so that PLGA can emit fluorescence at a certain excitation wavelength and use it to prepare dual fluorescent nanoparticles

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0041] Synthesis of the polylactic acid-glycolic acid copolymer material modified by embodiment 1 rhodamine B

[0042] Dissolve 150 mg of poly(lactic acid-glycolic acid) copolymer completely in 8 mL of dichloromethane, add 3.8 mg of N-hydroxysuccinimide, 110 μL of triethylamine and 6.2 mg of 1-ethyl-3 -(3-Dimethylaminopropyl)carbodiimide hydrochloride, gradually warming up to room temperature and reacting for 10 hours to obtain reaction solution A, add 5 μL ethylenediamine to 3.2mL dichloromethane solution, mix well, and then The dichloromethane solution of ethylenediamine was added dropwise to the reaction solution A, and reacted at room temperature for 20 hours. After the reaction was completed, the reaction system was washed successively with saturated aqueous sodium bicarbonate solution, distilled water and saturated aqueous sodium chloride solution, and then washed with anhydrous Sodium sulfate was dried overnight, filtered, and the filtrate was dried under reduced pressu...

Embodiment 2

[0044] Synthesis of the polylactic acid-glycolic acid copolymer material modified by embodiment 2 rhodamine B

[0045] Dissolve 200mg of poly(lactic acid-glycolic acid) copolymer completely in 10mL of dichloromethane, add 4.6mg of N-hydroxysuccinimide, 120μL of triethylamine and 7.8mg of 1-ethyl-3 -(3-Dimethylaminopropyl)carbodiimide hydrochloride, gradually warming up to room temperature and reacting for 12 hours, to obtain reaction solution A, add 6.7 μL ethylenediamine to 4mL dichloromethane solution, mix well, and then The dichloromethane solution of ethylenediamine was added dropwise to the reaction solution A, and reacted at room temperature for 23 hours. After the reaction was completed, the reaction system was washed successively with saturated aqueous sodium bicarbonate solution, distilled water and saturated aqueous sodium chloride solution, and then washed with anhydrous Sodium sulfate was dried overnight, filtered, and the filtrate was dried under reduced pressure ...

Embodiment 3

[0051] Example 3 Fluorescence Stability Investigation of Rhodamine B Modified Polylactic-Glycolic Acid Copolymer Material

[0052] In order to investigate whether the polylactic acid-glycolic acid copolymer modified by Rhodamine B can emit fluorescence, 5 mg of the polylactic acid-glycolic acid copolymer modified by Rhodamine B prepared in Example 2 was completely dissolved in dichloromethane to obtain a concentration of 0.5mg / mL rhodamine B-modified copolymer dichloromethane solution; take rhodamine B-modified polylactic acid-glycolic acid copolymer 0.4mL dichloromethane solution, add methanol to make a concentration of 4μg / mL rhodamine The methanol solution of the copolymer modified by B is detected by a fluorescence spectrophotometer, and the spectrogram is shown in figure 2 ; The rhodamine B modified polylactic acid-glycolic acid copolymer methanol solution was placed at 37°C and 4°C under natural light for a certain period of time to investigate the fluorescence stabilit...

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Abstract

The invention relates to a double-emitting fluorescent nanoparticle preparation method, which comprises: covalently linking rhodamine B and a polylactic acid-glycolic acid copolymer through an intermediate linking molecule ethylenediamine to prepare a biocompatible material Rhodamine B modified polylactic acid-glycolic acid copolymer with fluorescence emission function, and encapsulating a fluorescent tracer with the fluorescent biocompatible material to obtain the double-emitting fluorescent nanoparticles. According to the present invention, the preparation method has characteristics of simple and easily-available raw materials and mild reaction conditions, and the prepared double-emitting fluorescent nanoparticles have characteristics of smooth and complete surface, uniform particle sizedistribution, good stability and good dual fluorescence emission performance, can achieve visual monitoring, can be used in the fields of clinical diagnosis and drug evaluation, and has good application prospects.

Description

technical field [0001] The invention belongs to the technical field of nanometer preparation, and in particular relates to a method for preparing double-emission fluorescent nanoparticles. Background technique [0002] Polylactic acid-glycolic acid copolymer (PLGA) is a polymer material polymerized by lactic acid and glycolic acid according to a certain ratio, and its degradation products are lactic acid and glycolic acid, which are also human metabolites. Due to its good biocompatibility, biodegradability, stable quality, and adjustable degradation rate, it has been widely used as a carrier material for micro-nano controlled release and targeting systems in recent years. [0003] In order to characterize the behavior of nanoparticles in cells or in vivo, nanoparticles are usually loaded with a certain amount of fluorescent dyes to trace the transport and distribution behavior of the nanoparticles. However, the environment of cells or in vivo is complex. After release, this...

Claims

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Application Information

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IPC IPC(8): C08G63/91C09K11/02C09K11/06
CPCC08G63/912C09K11/025C09K11/06C09K2211/1088
Inventor 卢闻张杰贺浪冲杨晓莹窦桃艳王杰韩成坤
Owner XI AN JIAOTONG UNIV
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