Preparation method of highly ordered phospholipid vesicle array

A highly ordered, phospholipid technology, used in the preparation of test samples, etc., can solve the problems of disordered phospholipid vesicles and uneven size of phospholipid vesicles, and achieve simple and rapid preparation, short preparation time, and simple method. Effect

Active Publication Date: 2019-02-01
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to solve the problem that the phospholipid vesicles prepared by the existing large-area electrode are disordered and the size of the phospholipid vesicles is not uniform, and to provide a method for preparing a highly ordered phospholipid vesicle array

Method used

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  • Preparation method of highly ordered phospholipid vesicle array
  • Preparation method of highly ordered phospholipid vesicle array
  • Preparation method of highly ordered phospholipid vesicle array

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Experimental program
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specific Embodiment approach 1

[0025] Embodiment 1: This embodiment is a method for preparing a highly ordered phospholipid vesicle array, which is completed in the following steps:

[0026] 1. Electrode preparation and cleaning:

[0027] First, the striped microelectrode was prepared on the surface of ITO conductive glass by photolithography to obtain the striped microelectrode substrate; the striped microelectrode substrate was put into a sodium carbonate solution with a mass fraction of 1% to 1.5% and ultrasonically washed for 1min to 3min, and ultrasonically washed for 1min to 3min. The power is 250W ~ 300W, and then use distilled water to wash 2 to 3 times, and finally use nitrogen to dry to obtain the striped microelectrode substrate after cleaning;

[0028] The striped microelectrode described in step 1 is an array formed by a plurality of strip electrodes connected in parallel, wherein the width of each strip electrode is 10 μm to 50 μm, and the distance between every two strip electrodes is 10 μm t...

specific Embodiment approach 2

[0042] Specific embodiment two: the difference between this embodiment and specific embodiment one is: adopt photolithography described in step one to prepare striped microelectrode on the surface of ITO conductive glass and finish according to the following steps:

[0043]①. First, ultrasonically clean the ITO conductive glass in absolute ethanol at an ultrasonic power of 250W to 300W for 10min to 15min, then ultrasonically clean the ITO conductive glass in distilled water at an ultrasonic power of 250W to 300W for 10min to 15min before use Blow dry with nitrogen, and finally treat with a plasma cleaning machine for 25s~35s, then put it on a heating plate with a temperature of 105°C~115°C and heat it for 5min~15min to obtain a pretreated ITO conductive glass;

[0044] ②. First, use a plastic sealing machine with a temperature of 110°C to 115°C to press the photoresist on the pretreated ITO conductive glass to form a film, then place a mask with a pattern on the film, and then ...

specific Embodiment approach 3

[0049] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that in step 2 ①, the phospholipids are dissolved in chloroform to obtain a phospholipid solution with a concentration of 0.5 mg / mL-1 mg / mL. Other steps are the same as those in Embodiment 1 or 2.

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Abstract

The invention provides a preparation method of a highly ordered phospholipid vesicle array, and relates to a preparation method of phospholipid vesicles. The object of the invention is to solve the problems that the phospholipid vesicles prepared by an existing large-area electrode are disordered, and the sizes of the phospholipid vesicles are not uniform. The method comprises the following steps:first, preparation and cleaning of an electrode; second, coating and drying of a phospholipid dry film; third, assembly; and fourth, performing an electric vesicle forming process to obtain the highly ordered phospholipid vesicle array. By adoption of the preparation method provided by the invention, the simple and rapid preparation of the phospholipid vesicles with highly ordered arrangement anduniform size is creatively achieved, the preparation time is short, and the preparation method is suitable for industrial production. The invention provides the preparation method of the highly ordered phospholipid vesicle array.

Description

technical field [0001] The invention relates to a preparation method of phospholipid vesicles. Background technique [0002] As a closed matrix model of cells, phospholipid vesicles are excellent templates for analyzing the fine structure of cells and simulating the biological functions of cells, and are also excellent models for studying membrane osmotic pressure, membrane curvature and tension. Among them, the electroporation method is a common method to prepare phospholipid vesicles with cell size, but the phospholipid vesicles formed on the traditional planar substrate are disordered, which makes it difficult for experimental research that requires phospholipid vesicles to be dispersed and ordered. Contents of the invention [0003] The purpose of the present invention is to solve the problem that the phospholipid vesicles prepared by the existing large-area electrode are disordered and the phospholipid vesicles are not uniform in size, and provide a method for prepari...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
CPCG01N1/28
Inventor 毕洪梅史国滨
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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