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Lactic acid bacteria fermentation liquid rich in natural bromelain, and preparation method thereof

A technology of lactic acid bacteria fermentation broth and pineapple enzyme, which is applied in the application, animal feed, animal feed and other directions, can solve the problems of failing to reach the international advanced level and low viable bacteria, and achieve improved product value, high enzyme activity, and enhanced immunity. Effect

Pending Publication Date: 2019-02-12
湛江市粤凯生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In current production technology, oligosaccharides are often added to promote the proliferation of lactic acid bacteria, but the amount of live bacteria in the product is still low, failing to reach the international advanced level (10 10 ~10 12 cfu / g)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0018] The present invention is a kind of preparation method of the lactic acid bacteria fermented liquid that is rich in natural pineapple enzyme, comprises the following steps:

[0019] (1) Mix soybean molasses and water evenly and sterilize, and the water content in the mixed solution is 70-80wt%. Soybean molasses is a by-product obtained during the production of concentrated soybean protein, preferably with a total sugar content of more than 40% on a dry basis. In the present invention, the selected soybean molasses complex contains soybean oligosaccharides, soybean isoflavones and various trace elements, which has an auxiliary effect on the expression of the later effect of the product and is superior to other molasses.

[0020] (2) Add raw pineapple juice and lactic acid bacteria seed culture solution to the mixed solution obtained in step (1), the addition ratio of raw squeezed pineapple juice is 3 to 10% of the mixed solution, and the addition ratio of lactic acid bact...

Embodiment 1

[0026] (1) press the juice after cleaning and sterilizing the pineapple, and then perform centrifugation to obtain raw pineapple juice;

[0027] (2) Add 300 kilograms of soybean molasses per ton of tap water, stir and mix well, and pass through ozone to sterilize.

[0028] (3) Add pineapple juice to the sterilized mixed solution, add 80 kg of raw pineapple juice per ton of mixed solution and stir evenly, then add Lactobacillus seed solution, the number of live bacteria in the seed solution is 10 9 cfu / g, add 500ml of seed solution per ton.

[0029] (4) Anaerobic fermentation at a constant temperature of 37 degrees Celsius for 48 hours;

[0030] (5) Centrifuge the bacterial liquid after fermentation, bottle the separated fermented liquid, cover and seal it, leave it to stand for 7 days, and ship it.

[0031] Product testing indicators are shown in Table 1.

[0032] Table 1.

[0033] pH

Embodiment 2

[0040] In the step (2) of comparative example 1, add 100 kilograms of raw squeezed pineapple juices to every ton of mixed liquor simultaneously.

[0041] Other steps are the same as Comparative Example 1. The obtained product parameters are compared in Table 2.

[0042] Table 2.

[0043] project

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Abstract

The invention discloses lactic acid bacteria fermentation liquid rich in natural bromelain, and a preparation method thereof. The preparation method includes the steps of evenly mixing soybean molasses with water while sterilizing, and adding squeezed pineapple juice and lactic acid bacteria seed culture liquid, wherein the adding amount of the squeezed pineapple juice accounts for 3-10 per cent of the mixed solution, and the adding amount of the lactic acid bacteria seed culture liquid accounts for 0.3-0.5 per mill of the soy molasses mixed solution; performing constant-temperature anaerobicfermentation to obtain the lactic acid bacteria fermentation liquid rich in natural bromelain. The lactic acid bacteria fermentation liquid has the advantages that the pineapple juice is applied to lactic acid bacteria fermentation, so that the number of living bacteria of the lactic acid bacteria fermentation liquid can be improved remarkably; the product value of the lactic acid bacteria fermentation liquid can be increased, and the lactic acid bacteria fermentation liquid has high enzyme activity and organic acids, can allow the bromelain to play a role when being added into animal feed inthe subsequent stages and has the effects of stimulating appetite, repairing intestinal tracts, enhancing the immunity and promoting the growth of the farmed animals.

Description

technical field [0001] The invention relates to the technical field of biological fermentation, in particular to a preparation method of lactic acid bacteria fermented liquid rich in natural pineapple enzymes. Background technique [0002] Pineapple fruit is rich in protease, flavor substances and nutrients, and is mainly used to be processed into canned food and beverages, but the commodity value is low and the effect of pineapple enzymes has not been exerted. The application of pineapple juice in breeding feed can not only increase the growth rate of animals, improve breast milk nutrition, but also improve animal meat. However, because pineapple juice is easy to deteriorate without treatment, it is inconvenient to store and long-distance transportation, which affects its development and utilization. [0003] Lactic acid bacteria exist in the intestinal tract of humans and animals, which can help digestion, absorb nutrients, regulate electrolyte balance, and inhibit the rep...

Claims

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Application Information

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IPC IPC(8): A23K10/12
CPCA23K10/12
Inventor 林冲王亚军
Owner 湛江市粤凯生物科技有限公司
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