Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of method and application of forming thin film

A film and electrospinning technology, applied in biochemical equipment and methods, filament/thread forming, textiles and papermaking, etc., can solve problems such as easy blowing, poor film stability, and difficult control of strength

Active Publication Date: 2021-04-09
TSINGHUA UNIV
View PDF9 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation of phospholipid bilayers by this method is complex and resource-intensive, which is not conducive to large-scale production
[0004] Existing methods mostly use the method of coating to prepare phospholipid bilayers, first coat a layer of oil phase, then coat the phospholipid layer, and prepare the phospholipid bilayers by blowing bubbles with a pipette gun, although this method has simple procedures and is economical , but it is easy to blow and break, and the strength is difficult to control, which will consume a lot of time for the staff
Patent document CN105637081A discloses a method for forming a lipid bilayer for a nanopore sensing device, and patent document WO2008102121A1 discloses a method for forming a lipid bilayer through a pore. equipment to prepare phospholipid bilayers. Although this method realizes mechanized preparation, the membrane stability is poor, quality standard control is difficult, and resource consumption is too large

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of method and application of forming thin film
  • A kind of method and application of forming thin film
  • A kind of method and application of forming thin film

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0125] Example 1 Preparation of film 1 (chip 10% PS).

[0126] Preparation of the array chip: the whole process of making the array chip is operated in a clean room and a clean room facility. Glass is used as the substrate. The substrate is first cleaned with acetone and IPA ultrasonically to remove oil stains, and then cleaned with oxygen plasma , and finally use a 150°C hot plate to dry. After using electron beam evaporation to plate 10nm chromium and 100nm gold on the substrate, put it into a spin coater at 4000rpm, spin coat for 60s to form a layer of S1813 photoresist (MicroChem Corp.), and then use a hot plate for pre-baking at 95°C After 2 minutes, use a UV lithography machine to expose through the mask, remove the exposed substrate and put it in a positive photolithography development for 120s, then use gold and chromium etching solutions to remove metals other than the electrodes to form a metal layer, and finally use Acetone removes the S1813 photoresist as a chemic...

Embodiment 2

[0129] The preparation of embodiment 2 film 2 (chip 30%PS)

[0130] The preparation of the array chip is the same as in Example 1. Spinning: temperature 40°C, humidity 40%, horizontal distance 15cm, height 41cm, receiving speed 50r / min, translational speed 400mm / min. Stir the 30% PS solution magnetically for 3 hours, and place it for 24 hours; ultrasonically for 15 minutes, draw 3ml with a syringe equipped with a 21-gauge needle, and remove air bubbles. Click the fast-forward button on the control panel of the electrospinning machine to let the liquid spray out, so that the needle is filled with liquid, adjust the relevant parameters on the control panel, connect the negative roller, and wrap a layer of aluminum foil on the roller; adjust the voltage separately The positive voltage is 20KV, and the negative voltage is 2.5KV. When the voltage rises to the corresponding value, click Start. When the aluminum foil turns white obviously, click stop, and turn off the positive and ...

Embodiment 3

[0132] The preparation of embodiment 3 film 3 (chip 10%PS+0.1%CTAB)

[0133] The preparation of the array chip is the same as in Example 1. Spinning: temperature 40°C, humidity 40%, horizontal distance 15cm, height 41cm, receiving speed 50r / min, translational speed 400mm / min. Stir the 10% PS + 0.1% CTAB solution magnetically for 3 hours, and place it for 24 hours; ultrasonically for 15 minutes, draw 3ml with a syringe equipped with a 21-gauge needle, and remove air bubbles. Click the fast-forward button on the control panel of the electrospinning machine to let the liquid spray out, so that the needle is filled with liquid, adjust the relevant parameters on the control panel, connect the negative roller, and wrap a layer of aluminum foil on the roller; adjust the voltage separately The positive voltage is 20KV, and the negative voltage is 2.5KV. When the voltage rises to the corresponding value, click Start. When the aluminum foil turns white obviously, click stop, and turn ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
diameteraaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for forming a film, which comprises the following steps: (1) forming a network structure layer or a porous structure layer on the surface of a substrate with holes; (2) forming a film. The method for forming a thin film can be used to prepare a thin film for sequencing, and the preparation of the thin film for sequencing can save time, reduce cost, and greatly improve production efficiency. The invention also discloses the use of the thin film in biological sequencing and / or detection.

Description

technical field [0001] The invention belongs to the field of biological materials, and in particular relates to a method and application for forming a thin film. Background technique [0002] In 1996, Branton's group demonstrated for the first time that when the current drives single-stranded DNA through the α-hemolysin protein embedded in the phospholipid bilayer, the current will drop instantaneously, proving that nanopore proteins can be used for DNA detection. Biological nanochannels are the most natural nanodevices using transmembrane channels, and their pore sizes are generally only 1 to 10 nanometers, and can only accommodate one molecule (such as DNA, Micro-RNA, polypeptide, sugar, etc.) The aqueous solution system enables real-time, high-throughput, label-free ultrasensitive analysis of individual molecules. At present, the most widely used biological nanochannels are α-hemolysin, MspA nanochannels, Csgg nanochannels and SP1 nanochannels. These nanochannels enable...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): D04H1/728D01D5/00B05D7/02B05D7/24C12Q1/6869
CPCB05D7/02B05D7/24C12Q1/6869D01D5/003D01D5/0084D04H1/728C12Q2565/631
Inventor 白净卫杜娟娟陈志杰
Owner TSINGHUA UNIV