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Application of tilapia protein peptide to preparation of immune preparation

A technology of immune preparations and tilapia, which is applied in the direction of hydrolyzed protein components, peptide/protein components, medical preparations containing active ingredients, etc., and can solve problems such as environmental pollution and waste of resources

Inactive Publication Date: 2019-02-19
汕尾市维明生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, the scale of the tilapia processing industry is also continuously expanding, and the leftovers in the tilapia processing process are mostly buried and discarded as waste, resulting in a great waste of resources and directly polluting the environment

Method used

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  • Application of tilapia protein peptide to preparation of immune preparation
  • Application of tilapia protein peptide to preparation of immune preparation
  • Application of tilapia protein peptide to preparation of immune preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The preparation of the protein peptide of embodiment 1 tilapia

[0047] Method for enzymatically hydrolyzing tilapia protein peptides from tilapia leftovers:

[0048] (1) Put the leftovers of tilapia into boiling water and cook for 10 minutes, take it out and cool, and obtain the tilapia material.

[0049] (2) Pulverize the cooled tilapia material, wash and stir with a 40-mesh mesh frame, and repeat 3-4 times.

[0050] (3) Mix according to the ratio of the mass of crushed tilapia material to the volume of water of 1:5, use a colloid mill to refine the pulp, adjust the system temperature to 45°C-60°C, pH 7.0-9.0, add trypsin Mass concentration 0.1-0.3%, stirring enzymolysis for 1h, then adding flavor protease mass concentration 0.1-0.3%, stirring enzymolysis for 3-6h, heating at 80°C-95°C for 10-25min, centrifuging to get supernatant.

[0051] (4) Concentrate the supernatant by nanofiltration, decolorize the obtained nanofiltration concentrate with activated carbon, evap...

Embodiment 2

[0055] In this experiment, the immunopromoting activity of tilapia protein peptide was verified.

[0056] 1. Experimental raw materials and related reagents

[0057] The tilapia protein peptides used in this experiment are from Shanwei Weiming Biotechnology Co., Ltd. (batch number 20180508-1). The preparation method is as shown in Example 1. The content of oligopeptides: more than 95.1%, detected and analyzed by ESI-LCMS mass spectrometry Its molecular weight range: 470-1417Da (such as diagram 2-1 with 2-2 shown); high-glucose DMEM medium (article number SH30022.01), penicillin, and streptomycin were purchased from Hyclone Company of the United States; fetal bovine serum was purchased from BI Company; NO detection kit, CCK-8 kit, and cell lysate were purchased from The iNOS monoclonal antibody and goat rabbit secondary antibody were purchased from CST Company in Germany; the ECL luminescent liquid was purchased from Thermo Fisher Scientific Company; LPS and other commonly u...

Embodiment 3

[0085] Except for the batch of tilapia protein peptide purchased from Shanwei Weiming Biotechnology Co., Ltd. (the content of oligopeptide: more than 95.1%, and the molecular weight range of ESI-LCMS mass spectrometry: 470-1417Da), the rest were Same as Example 1. NO release see Figure 5 .

[0086] Example 3

[0087] Except for the batch of tilapia protein peptide purchased from Shanwei Weiming Biotechnology Co., Ltd. (the content of oligopeptide: more than 95.1%, and the molecular weight range of ESI-LCMS mass spectrometry: 470-1417Da), the rest were Same as Example 1. NO release see Image 6 .

[0088] Such as Image 6 with image 3As shown, the present invention uses different batches of commercially available tilapia protein peptides, all of which have the effect of increasing NO release, which means that the tilapia protein peptide has a good immune regulation effect.

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Abstract

The invention relates to the technical field of bioactive peptides and provides application of a tilapia protein peptide to preparation of an immune preparation. The tilapia protein peptide refers toenzymolysis polypeptide obtained by enzymolysis of tilapia protein. According to researches, the tilapia protein peptide is capable of effectively inducing RAW264.7 cell iNOS expression and increasingNO release amount, and due to dose dependence, the tilapia protein peptide is excellent in immunoregulatory activity and can be used as a raw material for the immune preparation. Further, according to the researches, the tilapia protein peptide has no evident influences on RAW264.7 cell activity under immunoregulatory dosage, namely the tilapia protein peptide has no cytotoxicity when giving playto an immunoregulatory effect. The tilapia protein peptide is preferably derived from enzymatic hydrolysates of tilapia leftovers, and a novel application approach is provided for recycling of tilapia byproducts.

Description

technical field [0001] The invention relates to the technical field of bioactive peptides, in particular to the application of a tilapia protein peptide in the preparation of immune preparations. Background technique [0002] Tilapia is commonly known as South Sea crucian carp, African larvae, and apple fish. It is native to Lake Tanganyika in Africa. It can survive in seawater and freshwater, and has excellent adaptability and strong fecundity. Tilapia has high protein content and good protein quality. It contains various amino acids needed by the human body, and also contains vitamin A, vitamin D and various minerals needed by the human body, and has rich nutritional value. [0003] At present, bioactive peptides have become a research hotspot in the food science community, and there are many studies on the use of tilapia to extract active peptides. Liang Yongjun et al. extracted collagen from tilapia skin to separate and prepare tilapia active peptides, which have good a...

Claims

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Application Information

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IPC IPC(8): A61K38/01A61P37/04
CPCA61K38/014A61P37/04
Inventor 韩庆国黄文浩苑泽堃
Owner 汕尾市维明生物科技有限公司
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