Porous carbon material prepared through self-modification of rhodococcus opacus and preparation method and application of material
A cloudy rhodococcus and porous carbon material technology, applied in the preparation/purification of carbon, hybrid capacitor electrodes, etc., can solve the problems of environmental pollution, strong corrosion, long culture time, etc., and achieve environmental friendliness, high specific capacitance, bacteria The effect of a short growth cycle
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Embodiment 1
[0047] (1) Inoculate the Rhodococcus opacus PD630 bacteria stored on the LB slope into LB liquid medium, and cultivate for 18 hours at a temperature of 30° C. to obtain the seed liquid of Rhodococcus opacus PD630; wherein the ratio of the components of the LB liquid medium is For: 10g of peptone, 5g of yeast powder, 10g of sodium chloride, 1L of distilled water; the LB slope is based on the above formula by adding 15g / L of agar;
[0048] (2) Centrifuge the Rhodococcus opacus PD630 seed solution obtained in the previous step at 8000rpm for 5 minutes, discard the supernatant, and collect the thalline;
[0049] (3) Inoculate the collected Rhodococcus opacus PD630 bacterium into a low-nitrogen glucose medium at 2% inoculum size (the volume of the transferred seed solution and the volume of the culture solution after inoculation), at a temperature of 30°C, at a natural pH, Cultivate for 24 hours, and centrifuge at 8000rpm to obtain bacterial cells; wherein the ratio of the componen...
Embodiment 2
[0060] (1) Cultivate according to steps (1) and (2) in Example 1 to obtain the seed liquid of Rhodococcus opacus PD630.
[0061] (2) Centrifuge the obtained Rhodococcus opacus PD630 seed solution at 8000rpm for 5 minutes, discard the supernatant, and collect the thalline;
[0062] (3) The collected Rhodococcus opacus PD630 cells were inoculated in a low-nitrogen glucose medium at a 10% inoculation amount, cultivated for 48 hours at a temperature of 30° C., at a natural pH, and centrifuged at 8000 rpm to obtain bacterial cells; wherein the low The composition ratio of nitrogen glucose medium is: glucose 5g / L, NH 4 Cl0.8g / L, MgSO 4 ·7H 2 O 1.0g / L, CaCl 2 2H 2 O0.015g / L, CoCl 2 ·6H 2 O 0.050mg / L, CuCl 2 2H 2 O0.0050mg / L, EDTA 0.25mg / L, FeSO 4 ·7H 2 O 0.50mg / L, H 3 BO 3 0.015mg / L, MnSO 4 ·H 2 O 0.020mg / L, NiC1 2 ·6H 2 O 0.010mg / L, ZnSO 4 ·7H 2 O0.40mg / L, FeNa-EDTA5.0g / L, NaMoO 4 ·H 2 O 2.0mg / L; K 2 HPO 4 2.14g / L, KH 2 PO 4 1.33g / L.
[0063] (4) Place the...
Embodiment 3
[0068] (1) Cultivate according to steps (1) and (2) in Example 1 to obtain the seed liquid of Rhodococcus opacus PD630.
[0069](2) Centrifuge the obtained Rhodococcus opacus PD630 seed solution at 8000rpm for 5 minutes, discard the supernatant, and collect the thalline;
[0070] (3) The collected Rhodococcus opacus PD630 cells were inoculated in a low-nitrogen glucose medium at a 5% inoculum size, cultivated for 24 hours at a temperature of 30° C., at a natural pH, and centrifuged at 8000 rpm to obtain bacterial cells; wherein the low The composition ratio of nitrogen glucose medium is: glucose 5g / L, NH 4 Cl1.2g / L, MgSO 4 ·7H 2 O 1.0g / L, CaCl 2 2H 2 O0.015g / L, CoCl 2 ·6H 2 O 0.050mg / L, CuCl 2 2H 2 O0.0050mg / L, EDTA 0.25mg / L, FeSO 4 ·7H 2 O 0.50mg / L, H 3 BO 3 0.015mg / L, MnSO 4 ·H 2 O 0.020mg / L, NiC1 2 ·6H 2 O 0.010mg / L, ZnSO 4 ·7H 2 O0.40mg / L, FeNa-EDTA5.0g / L, NaMoO 4 ·H 2 O 2.0mg / L; K 2 HPO 4 2.14g / L, KH 2 PO 4 1.33g / L.
[0071] (4) Place the Rhodo...
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