Preparation method and application of a kresoxim-like molecularly imprinted polymer
A molecular imprinting, polymer technology, applied in the directions of alkali metal compounds, chemical instruments and methods, ion exchange, etc., can solve problems such as limiting the use of MIPs, and achieve the effects of good selectivity, small particle size and strong adsorption capacity
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[0017] A method for preparing molecularly imprinted polymers of kresoxystrobin, using kresoxystrobin and pyraclostrobin as template molecules, using methacrylic acid as a functional monomer, and using trimethylolpropane trimethacrylate as crosslinking molecules. Linking agent for polymerization reaction, and then remove unpolymerized functional monomers, cross-linking agents and template molecules in the polymer to obtain the final product.
[0018] In the present invention, by selecting p-krysstrobin and pyraclostrobin as template molecules, molecularly imprinted polymer microspheres (MIP) with high "class" specificity and selectivity have been synthesized, and at the same time, it can be realized that p-krysstrobin and pyraclostrobin Detection of pyraclostrobin.
[0019] The selection of functional monomers has a great influence on the adsorption properties of molecularly imprinted polymers, which needs to be selected according to the structure and basic properties of templa...
Embodiment 1
[0042] Example 1 Preparation of Molecularly Imprinted Polymer
[0043] 1. In a 100mL ground glass bottle, add 0.8mmol kysstrobin, 1.2mmol pyraclostrobin, 8mmol functional monomer methacrylic acid and 75mL acetonitrile-toluene solution (7:3, v / v) Set 2h;
[0044] 2. Add 20mmol of trimethylolpropane trimethacrylate and 50mg of 2,2` azobisisobutyronitrile, ultrasonically mix, then pass high-purity nitrogen gas under ice-water bath conditions for 10min, and seal with a ground glass stopper. Polymerization in a water bath at 65°C for 28 hours;
[0045] 3. After cooling to room temperature, centrifuge at 3000r / min for 10min to obtain the precipitated polymer, and perform Soxhlet extraction with a mixed solution of acetic acid and methanol with a volume ratio of 1:9, the extraction time is 20h, and the temperature is 80°C;
[0046] 4. Shake elution with methanol, place the finally obtained polymer beads in a vacuum oven at 60°C and vacuum-dry to constant weight to obtain kresoxim-l...
Embodiment 2
[0047] Example 2 Preparation of Molecularly Imprinted Polymer
[0048] 1. In a 100mL ground glass bottle, add 1mmol kysstrobin, 0.8mmol pyraclostrobin, 4mmol functional monomer methacrylic acid and 65mL acetonitrile-toluene solution (7:3, v / v), and let stand at room temperature 2.5 hours;
[0049] 2. Add 40mmol of trimethylolpropane trimethacrylate and 45mg of 2,2` azobisisobutyronitrile, ultrasonically mix, then pass high-purity nitrogen gas under ice-water bath conditions for 15min, and seal with a ground glass stopper. Polymerization was carried out in a water bath at 72°C for 24 hours;
[0050] 3. After cooling to room temperature, centrifuge at 2000r / min for 8min to obtain the precipitated polymer, and perform Soxhlet extraction with a mixed solution of acetic acid and methanol with a volume ratio of 1:8, the extraction time is 24h, and the temperature is 85°C;
[0051] 4. Shake elution with methanol, place the finally obtained polymer beads in a vacuum oven at 60°C and...
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