A kind of rhizobacteria and application thereof for preventing and treating late blight of potato
A technology of potato late blight and bacteria, applied in the direction of bacteria, application, fungicides, etc., can solve the problems of reducing crop disease resistance, few growth-promoting bacteria, single function, etc., and achieve good application prospects and the effect of promoting growth
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Embodiment 1
[0037] The identification of embodiment 1 bacterial strain
[0038] The bacterial strain of the present invention is isolated from healthy potato rhizosphere soil by using Lb medium, and the number is FXP04. The morphological, physiological and biochemical characteristics of FXP04 were analyzed with reference to the method of Hamdali et al. (2011).
[0039] Its morphological characteristics are as figure 1 shown. In ISP2 medium, cultured at 28°C for 12 days, the surface of the colony was fluffy and powdery, the aerial hyphae were white, the hyphae in the base were light orange, and there was no soluble pigment ( figure 1 A); spore filaments are flexible, and spores are oval ( figure 1 B). In ISP3 medium, cultured at 28°C for 12 days, the surface of the colony was fluffy and powdery, the aerial hyphae were white, and the hyphae in the base were yellow, without soluble pigment ( figure 1 C). In ISP4 medium, cultivated at 28°C for 12 days, the surface of the colony is flocc...
Embodiment 2
[0050] Inhibition of Example 2 Streptomyces sp.FXP04 to Phytophthora infestans
[0051] Prepare rye solid medium, autoclave at 121°C for 20 minutes, pour it into a plate, place a 0.5 cm diameter Phytophthora infestans stipe in the center of the medium after solidification; A hole was drilled at 2.5 cm on both sides of the Phytophthora stipe, the diameter of the hole was 0.5 cm, and 100 microliters of Streptomycessp.FXP04 bacterial solution in the exponential growth phase was injected into the hole. Incubate in the dark at 21°C. Inhibition rate of Streptomyces sp.FXP04 against Phytophthora infestans = (diameter of colonies cultured in confrontation - diameter of colonies cultured alone) / diameter of colonies cultured alone * 100%.
[0052] From figure 1 It can be seen that when Phytophthora infestans is cultured alone, its colony grows uniformly in all directions, but the growth of Phytophthora infestans in the confrontation direction is severely inhibited in confrontation cul...
Embodiment 3
[0053] The effect of embodiment 3 Streptomyces sp.FXP04 on the growth of cucumber seedlings
[0054] Lb medium, adjust the pH to 7.2-7.4, inoculate the Streptomyces sp.FXP04 seed solution, culture in the dark at 30°C 120 rpm, cultivate to the exponential growth phase, centrifuge at 5000 rpm for 10 minutes, discard the supernatant, and use for precipitation Wash with sterile water, centrifuge again, discard the supernatant, resuspend the precipitate with sterile water, adjust OD 620 to 0.6 (through the dilution plate experiment, when the OD value is 0.6, the bacterial solution concentration is about 1.0×10 8 individual / mL, that is, 100 million / mL). The cucumber seeds were respectively in sterile water, the stock solution of the bacterial suspension with an OD value of 0.6 (hereinafter referred to as stock solution), the stock solution of 1 / 2 concentration (abbreviated 1 / 2), and the stock solution of 1 / 5 concentration (abbreviated 1 / 5), Soak the seeds for 30 minutes, cultivate...
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