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Detection method of thrombin based on metal ion coordination-molecular imprinted sensor

A metal ion, molecular imprinting technology, applied in the field of thrombin detection, can solve problems such as selectivity and sensitivity need to be improved

Active Publication Date: 2020-07-07
EAST CHINA JIAOTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, although molecularly imprinted electrochemical sensors have made great progress in the application research of biochemical analysis, environmental analysis, food and medicine, their selectivity and sensitivity need to be improved.

Method used

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  • Detection method of thrombin based on metal ion coordination-molecular imprinted sensor
  • Detection method of thrombin based on metal ion coordination-molecular imprinted sensor
  • Detection method of thrombin based on metal ion coordination-molecular imprinted sensor

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Preparation of metal ion coordination-molecularly imprinted sensors based on graphene / gold nanoparticles, the preparation steps are as follows figure 1 shown.

[0028] (1) Using graphite powder as raw material, graphene oxide was prepared by Hummers oxidation method.

[0029] (2) Drop 5 μL of 0.1 mg / mL graphene oxide onto the surface of a clean glassy carbon electrode, and let it dry naturally at room temperature. Then the modified electrode was placed in 0.1mol / L KCl solution, and scanned 20 times in the range of -1.8~0.4V to obtain a reduced graphene modified glassy carbon electrode.

[0030] (3) Put the reduced graphene modified glassy carbon electrode in 0.2-1.0mmol / L HAuCl 4 In the solution, the scanning speed is 20mV / s, the potential range is -1.1~0.2V, and scanning is performed 20 times to obtain a gold nanoparticle / reduced graphene modified glassy carbon electrode.

[0031] (4) Mix 50 μL of 0.2 mg / mL thrombin with 5 μL of 0.01 mol / L zinc nitrate solution and ...

Embodiment 2

[0035] Fabrication of metal ion coordination-molecularly imprinted sensors based on graphene / gold nanoparticles.

[0036] (1) Using graphite powder as raw material, graphene oxide was prepared by Hummers oxidation method.

[0037] (2) Drop 3 μL of 1.0 mg / mL graphene oxide onto the surface of a clean glassy carbon electrode, and let it dry naturally at room temperature. Then the modified electrode was placed in 0.1mol / L KCl solution, and scanned 40 times in the range of -1.8~0.4V to obtain a reduced graphene modified glassy carbon electrode.

[0038] (3) Put the reduced graphene modified glassy carbon electrode in 0.2mmol / L HAuCl4 In the solution, the scanning speed is 50mV / s, the potential range is -1.1~0.2V, and scanning is performed 5 times to obtain gold nanoparticles / reduced graphene modified glassy carbon electrodes.

[0039] (4) Mix 50 μL of 0.5 mg / mL thrombin with 5 μL of 0.10 mol / L nickel nitrate solution and incubate for 1 hour to obtain the nickel ion-thrombin compl...

Embodiment 3

[0043] Fabrication of metal ion coordination-molecularly imprinted sensors based on graphene / gold nanoparticles.

[0044] (1) Using graphite powder as raw material, graphene oxide was prepared by Hummers oxidation method.

[0045] (2) Drop 6 μL of 0.5 mg / mL graphene oxide onto the surface of a clean glassy carbon electrode, and let it dry naturally at room temperature. Then the modified electrode was placed in 0.1mol / L KCl solution and scanned 20 times in the range of -1.8~0.4V to obtain a reduced graphene modified glassy carbon electrode.

[0046] (3) Put the reduced graphene modified glassy carbon electrode in 0.5mmol / L HAuCl 4 In the solution, the scanning speed is 50mV / s, the potential range is -1.1~0.2V, and scanning is performed 15 times to obtain a gold nanoparticle / reduced graphene modified glassy carbon electrode.

[0047] (4) Mix 80 μL of 0.2 mg / mL thrombin with 15 μL of 0.05 mol / L copper sulfate solution and incubate for 2 hours to obtain a copper ion-thrombin com...

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Abstract

A method for detecting thrombin based on a metal ion coordination-molecular imprinting sensor, the method uses a metal ion coordination-molecular imprinting modified electrode as a working electrode, a reference electrode as an Ag / AgCl electrode, and an auxiliary electrode as a platinum electrode, consisting of The three-electrode system realizes the highly sensitive detection of thrombin. The metal ion coordination-molecularly imprinted modified electrode preparation method includes: (1) preparing graphene oxide by oxidation; (2) obtaining reduced graphene modified glassy carbon electrode; (3) obtaining gold nanoparticles / reduced graphene modified glassy carbon electrode; Carbon electrode; (4) Obtain transition metal-thrombin complex; (5) Prepare metal ion coordination-molecularly imprinted modified electrode, which is a sensor for detecting thrombin. The invention provides a method for sensitively detecting non-electroactive substance thrombin with an electrochemical sensor based on metal ion coordination and molecular imprinting.

Description

technical field [0001] The invention relates to a thrombin detection method based on a metal ion coordination-molecular imprinting sensor, which belongs to the technical field of chemical sensing and electroanalysis chemical detection. Background technique [0002] Thrombin is a serine proteolytic enzyme widely present in the blood coagulation system of mammals, which can catalyze the transformation of fibrinogen into fibrin in the body, and plays an extremely important role in wound healing, inflammation, blood coagulation, etc. It is commonly used in local hemostasis of capillary bleeding and tissue healing after surgery. The activity and concentration of thrombin is one of the important indicators to measure the health of the coagulation mechanism. very significant. [0003] In recent years, although molecularly imprinted electrochemical sensors have made great progress in the application research of biochemical analysis, environmental analysis, food and medicine, etc., ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30
CPCG01N27/308
Inventor 杨绍明易小梅刘海锋王少会腾渝柏朝朋张剑
Owner EAST CHINA JIAOTONG UNIVERSITY