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A Method for Evaluating the Effects of Electromagnetic Radiation on the Energy Metabolism of Organisms Using MFC
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A technology of energy metabolism and electromagnetic radiation, which is applied in microorganism-based methods, biochemical equipment and methods, and the determination/inspection of microorganisms. It can solve the problems of low experimental reproducibility, inconsistent experimental methods and experimental standards, and reliable experimental results. Questions such as reliability and credibility, to achieve the effect of sensitive response, good repeatability and simple operation
Active Publication Date: 2022-05-13
FUJIAN ACAD OF MEDICAL SCI
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However, so far, no biomarkers that can be used to indicate the effect of electromagnetic fields on the energy metabolism of organisms have been found
The main reason is that the above studies need to lyse the cells, so the experimental results cannot reflect the influence of electromagnetic fields on the energy metabolism of organisms in real time.
In addition, due to the inconsistency of experimental conditions (such as electromagnetic field strength), experimental methods, and experimental standards, the reproducibility of some experiments is low, leading to doubts about the reliability and credibility of experimental results
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[0029] The embodiments described below are exemplary and are intended to explain the present invention, but should not be construed as limiting the present invention.
[0030] Example 1:
[0031] (1) Strain cultivation: the electrogenic microorganismShewanella Oneida ( Shewane1la oneidensis MR-1, ATCC700550) were streaked and inoculated in solid LB medium (tryptone 10 g / L, yeast extract 5 g / L, NaCl 10 g / L, agarpowder 15 g, pH adjusted to 7.0), cultured at 30°C After 24 h, a single colony was picked and placed in liquid LB medium, 30°C, 180 rpm, and cultured for 12 h.
[0032] (2) Preparation of MFC inoculum solution: centrifuge the bacterial solution in (1) at 6000 r / min at 4°C for 2 min, remove the supernatant under anaerobic conditions, and obtain bacterial cells, use PBS buffer solution, the composition is ( / L): Na 2 HPO 4 12H 2 O 22.2 g; NaH 2 PO 4 2H 2 O5.93 g; KCl 0.13 g, pH7.0, washed three times, and resuspended in 0.5 mL to obtain MFC inoculum.
[0033] ...
Embodiment 2
[0039] (1) Strain cultivation: the electrogenic microorganismGeobactersulfur-reducing ( Geobactersulfur reducers PCA, DSMZ 12127) were inoculated in liquid NBAF medium (fumaric acid 4.64 g / L, NaHCO 3 2.50 g / L, NH 4 Cl 0.25 g / L, NaH 2 PO 4 2H 2 O 0.68 g / L, KCl 0.10 g / L, vitamin mother solution 10 ml / L, trace element mother solution 10 ml / L, sodium acetate 0.85 g / L, pH adjusted to 7.0), the inoculation amount of the bacterial solution is the amount of the inoculation medium 10%, cultured at 30°C for 18 h.
[0040] (2) Preparation of MFC inoculum solution: centrifuge the bacterial solution in (1) at 6000 r / min at 4°C for 2 min, remove the supernatant under anaerobic conditions, and obtain bacterial cells, use PBS buffer solution, the composition is ( / L): Na 2 HPO 4 12H 2 O 22.2 g; NaH 2 PO 4 2H 2 O5.93 g; KCl 0.13 g, washed three times, and resuspended in 0.5 mL to obtain the MFC inoculum.
[0041] (3) Preparation of MFC electrode solution: MFC catholyte is PBS ...
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technical field [0001] The invention belongs to the field of microbial fuel cells, and in particular relates to a method for evaluating the influence of electromagnetic radiation on the energy metabolism of organisms by using MFC. Background technique [0002] The process of electromagnetic field propagating in space at a certain speed is electromagnetic radiation. Electromagnetic radiation is an objective existence. As a kind of energy flow, human beings cannot directly feel it, but it is present all the time and everywhere. When the intensity of electromagnetic radiation exceeds the standard of environmental protection, it will produce negative effects and cause different lesions and hazards of the human body. This part of the radiation exceeding the standard electromagnetic field intensity is called electromagnetic radiation pollution. Different from chemical pollution and biological pollution, electromagnetic radiation pollution is energy pollution, and there will be no...
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