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Method of measuring contents of various organic acids in tobacco and tobacco products.

A technology of tobacco products and organic acids, which is applied in the field of tobacco analysis, can solve the problems of inappropriate low molecular weight organic acid analysis, difficult separation and accurate quantitative analysis, etc., and achieve the effect of high sensitivity and variety

Active Publication Date: 2019-03-01
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In this method, due to the aqueous phase system after saponification, it is not suitable for silanization, and the derivatization method of methyl esterification is used, but the derivatization method of methyl esterification is not suitable for the analysis of low molecular weight organic acids.
[0004] Due to the limitations of methods and instruments in the above detection methods, many kinds of organic acids and organic acid esters, especially low molecular weight organic acids, have not been detected and analyzed.
Among them, low-molecular-weight organic acids are difficult to effectively separate and accurately quantitatively analyze them from higher fatty acids at the same time due to their strong water solubility and strong volatility.

Method used

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  • Method of measuring contents of various organic acids in tobacco and tobacco products.
  • Method of measuring contents of various organic acids in tobacco and tobacco products.
  • Method of measuring contents of various organic acids in tobacco and tobacco products.

Examples

Experimental program
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Effect test

Embodiment 1

[0077] The method for measuring the content of various organic acids in tobacco or tobacco products of the present embodiment comprises the following steps:

[0078] (1) A typical C3F flue-cured tobacco leaf is ground into powder, and then passed through a 40-mesh sieve. Then weigh 1.0g smoke powder into a 50mL round bottom flask, then add 50mL 0.5mol / L NaOH methanol solution and 200μL 500μg / mL trans-2-hexenoic acid methanol solution in turn, reflux at 65°C for 2h, filter with suction, and take Filtrate 25mL, concentrated to dryness under reduced pressure, added 1.0mL water to redissolve to obtain solution A;

[0079] (2) Transfer solution A to a 50mL centrifuge tube, then add 1.0mL concentrated phosphoric acid (85% mass fraction) dropwise in an ice bath, oscillate, and adjust the pH to about 2.0. Then add 15mL of dichloromethane solution, and vortex for 10min;

[0080] (3) Take 1.0 mL of dichloromethane phase, add 200 μL of bis(trimethylsilyl)trifluoroacetamide (BSTFA) deri...

Embodiment 2

[0083] The method for measuring the content of various organic acids in tobacco or tobacco products of the present embodiment comprises the following steps:

[0084] (1) Stripping a typical finished cigarette sample, collecting shredded tobacco and grinding it into powder, the sample is ground into powder, and then passed through a 40-mesh sieve. Then weigh 1.0g smoke powder into a 50mL round bottom flask, then add 50mL 0.5mol / L NaOH methanol solution, 200μL 500μg / mL trans-2-hexenoic acid methanol solution, reflux at 65°C for 2h, filter with suction, and take the filtrate 25mL, concentrated to dryness under reduced pressure, redissolved in 1.0mL water to obtain solution A;

[0085] (2) Transfer solution A to a 50mL centrifuge tube, then add 1.0mL concentrated phosphoric acid (85% mass fraction) dropwise in an ice bath, oscillate, and adjust the pH to about 2.0. Then add 15mL of dichloromethane solution, and vortex for 10min;

[0086] (3) Take 1.0 mL of dichloromethane phase,...

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Abstract

The invention belongs to the technical field of tobacco analysis and particularly relates to a method of measuring contents of various organic acids in tobacco and tobacco products. The method hereinincludes the steps of mixing well a sample under test, a saponifying agent and an internal standard substance, refluxing at 60-70 DEG C, performing solid-liquid separation, concentrating filtrate under reduced pressure to dryness, and dissolving in water to obtain solution A; adjusting pH of the solution A at 0 DEG C to 1.5-2.5, and adding an extracting agent to carry out extracting; adding a silanized derivative into extract to carry out derivation, and using gas chromatography tandem mass spectrometry to perform detection and analysis. A saponifying-silanization-GC-MS / MS analytic method is established for the first time herein and is suitable for analyzing 53 amino acids in an analysis sample; the target analytes include low molecular weight organic acids, half-volatile organic acids andadvanced fatty acids, and the types are more in quantity and more comprehensive. GC-MS / MS analysis parameter sensitivity is higher; the low contents of organic acids in tobacco and tobacco products can be detected.

Description

technical field [0001] The invention belongs to the technical field of tobacco analysis, in particular to a method for measuring the contents of various organic acids in tobacco and tobacco products. Background technique [0002] Organic acids widely exist in tobacco, with a content of 10% to 16%, and play an important role in the aroma and taste of cigarettes. At the same time, the content of organic acid esters in tobacco leaves is also relatively high, which also plays an important role in the sensory quality of cigarettes. For example, low-level fatty acid esters have their own fragrance, while high-level fatty acid esters will crack corresponding acids during the smoking process of cigarettes. Cigarette smoke aroma. Therefore, it is of great significance to establish a method that can simultaneously analyze organic acids and organic esters in tobacco leaves for evaluating the style and quality of tobacco leaves. [0003] In "Optimization of Saponification Conditions a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88
Inventor 余晶晶王冰蔡君兰李东亮张艇秦亚琼崔华鹏陈黎刘绍锋张晓兵
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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