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Root knot nematode disease prevention pseudomonas strain Jdn1 and microbial agent thereof

A technology of root-knot nematode disease and pseudomonas, applied in the field of microorganisms, can solve the problems of lack of root-knot nematode resistance, etc., and achieve the effects of saving costs, reducing the number of root-knots, and reducing the number of root-knots

Inactive Publication Date: 2019-03-05
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is a lack of Pseudomonas strains and bacterial agents for preventing and controlling root-knot nematode disease that are not easy to cause root-knot nematodes to develop resistance and do not pollute the environment

Method used

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  • Root knot nematode disease prevention pseudomonas strain Jdn1 and microbial agent thereof
  • Root knot nematode disease prevention pseudomonas strain Jdn1 and microbial agent thereof
  • Root knot nematode disease prevention pseudomonas strain Jdn1 and microbial agent thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Physiological and biochemical tests of strains and identification of 16S rRNA molecules

[0027] Such as figure 1 As shown, the Pseudomonas strain Jdn1 was activated and cultured on the NA plate at a temperature of 28°C, NA medium: 3g of beef extract (Beef Extract), 10g of peptone (Peptone), 5g of sodium chloride (NaCl), Agar powder (Agar) 15g, pH7.0.

[0028] After culturing for 20-24 hours, it was observed that the single colony of Jdn1 was light yellow, nearly round, transparent, with neat edges and smooth surface. Gram staining of strain Jdn1 is red, which is Gram-negative bacteria. In the aerobic test, the strain Jdn1 grew along the surface of the culture medium, which indicated that the strain Jdn1 was an aerobic bacterium. Physiological and biochemical tests are oxidase, catalase, propionic acid, acetic acid, formic acid, L-lactic acid, L-malic acid, citric acid, α-ketoglutarate, α-D-glucose, D-mannose, sucrose, glycerol All were positive; gelatin liquefactio...

Embodiment 2

[0033] The preparation method steps of root-knot nematode second instar larvae are as follows:

[0034] (1) Cut the tomato root into pieces and put it into a bottle, add an appropriate amount of 20% sodium hypochlorite solution for immersion, and shake vigorously for 3 minutes.

[0035] (2) Pour the tomato roots into the standard sieves (30 mesh, 100 mesh, 300 mesh, 500 mesh) listed in descending order of sieve density, and wash the eggs onto the 500-mesh standard sieve and collect them.

[0036] (3) The collected eggs are sterilized with 10% sodium hypochlorite solution and washed with sterilized water.

[0037] (4) Put the sterilized nematode eggs into a sterilized device to hatch, and start to collect the hatched nematodes for later use.

[0038] The Pseudomonas bacterial strain of preventing and treating root-knot nematode disease of the present invention, this bacterial strain is Pseudomonas (Pseudomonassp.); Preservation name is Pseudomonas Jdn1; Preserved in China Micr...

Embodiment 3

[0050] Determination of potted control effect

[0051] Irrigate OD to the roots of 3-week-old tomato seedlings 600 For 1 Pseudomonas inoculum, each strain was filled with 10ml. Seedlings poured with an equal volume of sterilized water at the root were used as controls. One week later, 600 nematodes were inoculated on the roots of tomato seedlings. 8 seedlings per treatment, repeated 3 times. After 2 weeks of culture at 25°C, 16h: 8h (light: dark), count the number of root knots and calculate the control effect.

[0052] Control effect (%)=[(control group root-knot number-treatment group root-knot number) / control group root-knot number]×100%

[0053] Table 1 is the determination of potted control effect of Pseudomonas Jdn1 bacterial agent on root-knot nematode disease;

[0054] Table 1

[0055]

[0056] As shown in Table 1, it can be concluded that after 2 weeks of inoculation with 600 root-knot nematodes, the pot experiment results showed that the number of root-knots...

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Abstract

The invention discloses a root knot nematode disease prevention pseudomonas new species. The strain is a pseudomonas with the collection name of pseudomonas Jdn1 and collected in a China General Microbiological Culture Collection Center (CGMCC) at No. 3, yard 1, Beichen west road, Chaoyang district, Beijing on June 9, 2017, and the collection number of the strain is 14226. The single colony of thepseudomonas is faint yellow, nearly circular, transparent, neat in edge and smooth in surface, the pseudomonas strain is gram-stained to be red and is a Gram negative bacterium, and the pseudomonas strain grows along the surface of a culture medium in an aerobic test result and is an aerobic bacterium. The pseudomonas strain and the microbial agent thereof are good in root knot nematode disease prevention effects and environmentally friendly.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a new species of Pseudomonas sp. capable of preventing and treating root-knot nematode and its bacterial agent. Background technique [0002] Since Berkeley in 1855 in a greenhouse in England, it was found that the roots of diseased tomato plants had root knots. Since then, due to the convenience of transportation, the globalization of trade, and the rapid development of the economy, the spread and damage of root-knot nematodes have spread across space and spread all over the world. Diseases caused by root-knot nematodes cost the world more than $100 billion in annual economic losses. There are many types of root-knot nematodes, and there are four main types that cause serious damage to crops, namely, southern root-knot nematode (Meloidogyne incognita), peanut root-knot nematode (Meloidogyne arenaria), Java root-knot nematode (Meloidogyne javcmica) and norther...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P5/00C12R1/38
CPCA01N63/10C12N1/20C12N1/205C12R2001/38
Inventor 魏利辉张金凤周冬梅聂国嫒卢晓雪赵添润
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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