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Bacteria cinerea prevention actinomycete strain W15 and actinomycete agent thereof

A technology of botrytis cinerea and actinomycetes, applied in the field of microorganisms, to achieve the effect of reducing and saving costs

Inactive Publication Date: 2019-03-05
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is a lack of an actinomycete strain and an actinomycete agent for preventing and treating Botrytis cinerea that is not easy to cause resistance to Botrytis cinerea and does not pollute the environment.

Method used

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  • Bacteria cinerea prevention actinomycete strain W15 and actinomycete agent thereof
  • Bacteria cinerea prevention actinomycete strain W15 and actinomycete agent thereof
  • Bacteria cinerea prevention actinomycete strain W15 and actinomycete agent thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Identification of the 16S rRNA molecule of the strain

[0024] Actinomycetes strain W15 was activated and cultured on Gaoshi No. 1 plate at a temperature of 28°C. Gaoshi No. 1 medium: soluble starch 19.9-20.1g, sodium chloride 0.49-0.51g, potassium nitrate 0.9-1.1g , dipotassium hydrogen phosphate 0.49-0.51g, magnesium sulfate 0.49-0.51g, ferrous sulfate 0.009-0.011g, agar 15-20g composition, pH is 7.2-7.4, the above components are distilled to 1000ml with distilled water.

[0025] After culturing for 2-4 days, it was found that the single colony of W15 was round, brick red, and the surface was dry and opaque; the mycelium was thin, the growth was slow, and the colony was dense in texture and had a muddy smell. Gram staining of strain W15 was purple and positive.

[0026] The morphological characteristics of strain W15 were most similar to those of Streptomyces sp. in the identification manual of common bacterial systems.

[0027] Using the total DNA of the W15 genome...

Embodiment 2

[0030] The invention relates to an actinomycete strain for preventing and treating Botrytis cinerea, the strain is Streptomyces sp., and the name is Actinomyces W15.

[0031] The single colony of the actinomycete strain is round, brick red, and the surface is dry and opaque; the hyphae are thin, the growth is slow, and the colony is dense in texture and has a muddy smell. The Gram staining of the actinomycetes strain is purple, which is positive.

[0032] The actinomycete bacterial agent for preventing and treating Botrytis cinerea prepared by the actinomycete of the present invention. Its active ingredient is at least one of the following (a), (b) and (c):

[0033] (a) actinomycetes described in claim 1;

[0034] (b) the fermentation supernatant of actinomycetes described in claim 1;

[0035] (c) the fermentation culture of actinomycetes described in claim 1.

[0036] The preparation method of the actinomycetes bacterial agent of preventing and treating botrytis cinerea o...

Embodiment 3

[0047] Determination of control effect of detached leaves

[0048] Cultivate the actinomycetes until the predetermined time for use. Healthy leaves of the same size were collected from neatly growing tomato plants, soaked in 0.5% Tween 20 for about 10 seconds, rinsed gently with sterile water, took them out and dried them in an ultra-clean workbench. After the leaves are dried, soak them in the actinomycetes agent for 1 min, take them out and dry them in the ultra-clean workbench (leaves soaked in the supernatant of the actinomycetes fermentation medium are used as a control), and inoculate the leaves with tomato ash with a diameter of 5 mm. For fungus fungi, each treatment was repeated 8 times. At 25°C, relative humidity 80-90%, and dark conditions for 48 hours, measure the diameter of the lesion and evaluate the control effect. The formula for calculating the control effect is: control effect (%)=(the lesion diameter of the control group−the lesion diameter of the treatmen...

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Abstract

The invention discloses a bacteria cinerea prevention actinomycete strain, and belongs to the technical field of plant protection. The strain is a Streptomyces sp. and named as an actinomycete W15. The single colony of the actinomycete strain is circular and brick-red, the surface of the strain is dry and non-transparent, hyphae are thin and slowly grow, and the colony is compact in texture and has muddy and fishy smell. The actinomycete strain is gram-stained to be purple and positive. The prevention effect of the W15 agent on gray mold is 60% or more. Bacteria cinerea cannot easily generateresistance, the bacteria cinerea prevention actinomycete strain and the actinomycete agent thereof are environmentally friendly, and the agent can be used by root-irrigation after tomatoes germinate.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to an actinomycete strain W15 for preventing and treating Botrytis cinerea and an actinomycete bacterial agent thereof. Background technique [0002] Tomato is one of the most important vegetables in agricultural production. Botrytis cinerea caused by Botrytis cinerea is a worldwide disease that endangers tomato production. Botrytis cinerea is a fungal disease caused by Botrytis cinerea, which has become a worldwide fungal disease. Botrytis cinerea can harm leaves, flowers and fruits, among which fruit is the most important, and the fruit rot rate in seriously diseased fields can reach 40%. Botrytis cinerea can infect 235 kinds of vegetables, fruit trees and other economic crops. Once it becomes ill, it will cause a large amount of production reduction or even no harvest, causing great harm to agricultural production. [0003] Botrytis cinerea is a common disease of crops in open fie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/465
CPCA01N63/00C12N1/20C12R2001/465C12N1/205
Inventor 魏利辉卢晓雪张金凤冯辉陈曦
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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