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Antidote for treating blood circulation snake venom and preparation method thereof

A technology for antidote and snake venom, applied in the field of medicine, can solve the problems of high price of antivenom, inconvenient to store for a long time, and infrequent storage, etc., to achieve easy storage and portability, low cost, and low requirements for storage conditions. Effect

Inactive Publication Date: 2019-03-08
CHONGQING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, antivenom is considered to be an effective drug for treating snakebite, but antivenom is not perfect. Because antivenom is expensive and not easy to store for a long time, general hospitals do not often stockpile it, and once a person is bitten by a snake Immediate medication is required after injury, and it is difficult to treat in the first place in mountainous areas or underdeveloped areas with inconvenient transportation; at the same time, antivenom is prepared by immunizing animals, and miscellaneous proteins in serum may cause side effects, such as allergies Sexual shock, pyrogenic reaction and serum sickness, etc., so there are great limitations in application

Method used

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  • Antidote for treating blood circulation snake venom and preparation method thereof
  • Antidote for treating blood circulation snake venom and preparation method thereof
  • Antidote for treating blood circulation snake venom and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: 2.5g of ethyl gallate was mixed with 100g of water to make an injection. The venom of Agkistrodon akistrosus is a blood circulation-like snake venom, so Viper akistros venom was selected for experimental proof.

[0023] 1. Inhibitory effect of ethyl gallate on subcutaneous hemorrhage in mice induced by Agkistrodon venom

[0024] 30 Kunming mice (20±2) g were randomly divided into 5 groups. Each mouse in the snake venom group was subcutaneously injected with 100 μL Agkistrodon venom (0.2 μg / μL); each mouse in the control group was injected with 100 μL normal saline. Three hours after the injection, the animals were killed by deep anesthesia with ether, and the subcutaneous hemorrhage was observed by dissection.

[0025] Inhibition test: different mass ratios of snake venom and ethyl gallate (m(snake venom):m(ethyl gallate), 1:25, 1:50, 1:100) were pre-incubated at 37°C for 30 minutes, and then followed the above method for activity detection. The hemorrhagic...

Embodiment 2

[0037] 1. Preparation: Dissolve 20g of ethyl gallate, 2.5g of tea polyphenols, 1.0g of tannic acid, 2.5g of vitamin C, and 3.0g of disodium edetate in 1000g of water and mix evenly .

[0038]2. Experiment a: Select 40 Kunming male mice (20±2) g, divide them into 5 groups at random, and inject 100 μL of Agkistrodon venom (0.5, 1.0, 1.5, 2.0, 2.5 mg / kg), observe the number of dead mice within 24 h. Statistical analysis of the half-lethal dose of Agkistrodon halys venom was performed using the Pbobit module of SPSS software. To study the lethal effect of neutralizing snake venom, 20 Kunming male mice (20±2) g were selected and randomly divided into 2 groups. Mice were subcutaneously injected with 100ul 2LD50 Agkistrodon venom, and 200ul of this antidote was injected 5 minutes later for treatment. A normal saline treatment group was also set as a control, and the survival status of the mice within 24 hours was recorded.

[0039] Experiment b: 40 Kunming male mice (20±2) g were...

Embodiment 3

[0044] 1. Preparation: Dissolve 25g of ethyl gallate, 1.5g of tea polyphenols, 1.25g of tannic acid, 2.0g of vitamin C, 4.0g of disodium edetate in 1000g of water and mix evenly .

[0045] 2. Experiment a: Select 40 Kunming male mice (20±2) g, divide them into 5 groups at random, and inject 100 μL of Agkistrodon venom (0.5, 1.0, 1.5, 2.0, 2.5 mg / kg), observe the number of dead mice within 24 h. Statistical analysis of the half-lethal dose of Agkistrodon halys venom was performed using the Pbobit module of SPSS software. To study the lethal effect of neutralizing snake venom, 20 Kunming male mice (20±2) g were selected and randomly divided into 2 groups. Mice were subcutaneously injected with 100ul 2LD50 Agkistrodon venom, and 200ul of this antidote was injected 5 minutes later for treatment. A normal saline treatment group was also set as a control, and the survival status of the mice within 24 hours was recorded.

[0046] Experiment b: 40 Kunming male mice (20±2) g were s...

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Abstract

The invention discloses an antidote for treating blood circulation snake venom. The antidote comprises the raw material of ethyl gallate. The preparation method of the antidote comprises the steps offully dissolving 20-25 parts by weight of ethyl gallate, 1.5-2.5 parts by weight of tea polyphenol, 1.0-1.25 parts by weight of tannic acid, 2.0-2.5 parts by weight of vitamin C, 3.0-4.0 parts by weight of ethylenediamine tetraacetic acid disodium salt in 1000 parts by weight of water; and uniformly mixing the substances to obtain the antidote; or fully dissolving 20-25 parts by weight of ethyl gallate in 1000 parts by weight of water; and uniformly mixing the substances to obtain the antidote. According to the antidote and the preparation method thereof disclosed by the invention, the prepared antidote can effectively detoxify the blood circulation snake venom; the preparation method is simple; the cost is lower; the requirement for a storage condition is not high; and the antidote can bestored at normal temperature, is easy to store and carry, can be stored by an individual or a hospital and does not have side effects.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to an antidote for treating blood circulation snake-like venom and a preparation method thereof. Background technique [0002] After being poisoned by blood circulation toxins, they act on the blood system and can cause serious local damage in a short period of time, causing bleeding, edema, and necrosis of muscle tissue. If not treated in time, it will lead to permanent disability or death. At present, antivenom is considered to be an effective drug for treating snakebite, but antivenom is not perfect. Because antivenom is expensive and not easy to store for a long time, general hospitals do not often stockpile it, and once a person is bitten by a snake Immediate medication is required after injury, and it is difficult to treat in the first place in mountainous areas or underdeveloped areas with inconvenient transportation; at the same time, antivenom is prepared by im...

Claims

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Application Information

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IPC IPC(8): A61K36/82A61K31/353A61K31/7024A61K31/375A61K31/198A61P39/02
CPCA61K31/198A61K31/353A61K31/375A61K31/7024A61K36/82A61P39/02A61K2300/00
Inventor 和七一余晓东
Owner CHONGQING NORMAL UNIVERSITY
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