8 results about "Agkistrodon halys" patented technology

The invention discloses a method for separating and purifying hemocoagulase from snake venom of agkistrodon halys at Changbai Mountain. The method comprises the following steps: sequentially carrying out CAPTO SP ImpRes cation column chromatography and SOURCE 15 PHE hydrophobic column chromatography on a Changbai Mountain agkistrodon halys venom solution, so as to obtain the hemocoagulase. According to the method disclosed by the invention, purification is carried out by adopting different purification principles of cation chromatography and hydrophobic chromatography so as to remove impure protein residues; the problem of few impure protein residues is solved; by improving the chromatography technology and conditions, the use of precipitation, dialysis and other technologies is avoided, and next chromatography is carried out by adjusting the conductivity, the pH value and the like, so that the activity loss is reduced, and the production period is shortened; the method provided by the invention has the advantages of large one-time chromatography sample loading amount, high yield and high purity, and is beneficial to industrial production.

The artificial breeding device comprises a breeding pond (1), a plurality of temporary breeding ponds (2) are arranged on one side of the breeding pond (1), a plurality of adaptation ponds (3) are arranged on one side of the interior of the breeding pond (1), a venom taking chamber (6) is connected to the outer side of the breeding pond (1), a temporary storage chamber (7) is arranged on one side of the venom taking chamber (6), and a sampling opening, an operation window and a transparent observation window are formed in the venom taking chamber (6). Ecological environment simulation modules are arranged in the breeding pond (1) and the temporary breeding pond (2) respectively, and each ecological environment simulation module comprises a land simulation part, a water source simulation part, an illumination simulation part, a temperature simulation part, a ventilation part and a humidity simulation part. According to the breeding method, by dynamically simulating the wild growth environment of the agkistrodon halys, the survival rate of the agkistrodon halys is improved, batch breeding of the agkistrodon halys can be achieved in the feeding pond, the breeding efficiency is improved, the breeding cost is reduced, breeding, growth and poison taking of the agkistrodon halys are all conducted in the artificial breeding device, bacteria entering the artificial breeding device are reduced, diseases are reduced, and the survival rate of the agkistrodon halys is improved. The viper venom taking operation is convenient and safe.

This invention pertains to the field of *Snakehead sinensis*, specifically relating to its characteristic polypeptides and their applications. Specifically, it covers the characteristic polypeptides of *Snakehead sinensis*, a mass spectrometry-based method for authenticity identification, and the application of these characteristic polypeptides in the preparation of drugs for treating rheumatoid arthritis. This invention provides a characteristic polypeptide, QDVDNASLVR, which is found only in *Snakehead sinensis* and can be stably detected. Its amino acid sequence is not reported in the literature, making it a novel polypeptide. An LC-MRM-MS method for authenticity identification based on this characteristic polypeptide is established, achieving accurate differentiation from *Snakehead rubrum* and *Agkistrodon halys*, with a 100% authenticity identification rate. This invention also provides a characteristic polypeptide, SFLGVPFAK, with anti-rheumatoid arthritis activity. Its amino acid sequence is not reported in the literature, making it a novel polypeptide. Its target and pathway can be predicted, or it can be applied to the modification of anti-rheumatoid arthritis active polypeptides.

The invention relates to the field of traditional Chinese medicine preparations and the technical field of medicine analysis, in particular to a traditional Chinese medicine composition for treating liver cancer and intestinal cancer and a preparation method and a detection method thereof. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 200 to 300 parts of herba taraxaci, 200 to 300 parts of herba rabdosiae, 250 to 350 parts of ganoderma lucidum spore powder, 50 to 150 parts of solanum lyratum, 250 to 350 parts of centipede, 200 to 300 parts of radix scutellariae, 250 to 350 parts of oldenlandia diffusa, 250 to 350 parts of herba andrographitis, 400 to 500 parts of phellinus igniarius, 250 to 350 parts of radix tetrastigme, 50 to 200 parts of Chinese mahonia and 100 to 250 parts of agkistrodon halys. The traditional Chinese medicine composition has the effects of clearing away heat and toxic materials, removing blood stasis and phlegm, eliminating sores and removing stasis and is used for treating liver cancer and intestinal cancer.

The invention relates to an external traditional Chinese medicine ointment composition for treating lumbar disc herniation, which can effectively solve the problems of slow effect and limited effect of the existing medicine for treating lumbar disc herniation. The traditional Chinese medicine is prepared from frankincense, asarum, borneol, alangium platanifolium, radix aconiti kusnezoffii, herba chloranthi serrati, radix aconiti, short-pedicel aconite root, herba taxilli, kadsura longepedunculata, radix angelicae pubescentis, garden balsam stem, lycopodium clavatum, radix rehmanniae, prepared rehmannia root, radix dipsaci, safflower, rhizoma smilacis glabrae, cuttlebone, angelica sinensis, sappan wood, paris polyphylla, sea horse, semen momordicae, semen strychni, anisodus acutangulus, astragalus membranaceus, pseudo-ginseng, rhizoma drynariae, herba epimedii, obscured homalomena rhizome, eucommia ulmoides, kadsura pepper stem, acanthopanax, schizophragma integrifolium, the black plaster is prepared from bighead atractylodes rhizome, cinnamon, rhizoma atractylodis, codonopsis pilosula, poria cocos, gentiana macrophylla, hairyvein agrimony, radix sophorae flavescentis, fructus kochiae, common carpesium fruit, coptis chinensis, scutellaria baicalensis, golden cypress, rheum officinale, honeysuckle, radix clematidis, radix paeoniae rubra, long-noded pit viper, ground beetle, liquidambar formosana hance and bulbus fritillariae cirrhosae according to a conventional black method. The traditional Chinese medicine composition has the effects of activating blood, relieving pain, removing stasis and diminishing swelling.

The invention discloses application of agkistrodon halys to preparation of drugs or preparations for simulating vagina prebiotics and balancing vagina beneficial bacteria and harmful bacteria. The invention relates to the field of research of probiotics and prebiotics, aims to recover and maintain microecological balance of vagina and improve natural defense capability of vagina, and particularly relates to a method for preparing a medicine or a preparation capable of effectively balancing beneficial bacteria and harmful bacteria of vagina by taking agkistrodon halys as a prebiotic simulant (mimetic) with homology of medicine and food. The research proves for the first time that the water extract of the agkistrodon halys can promote the colonization of beneficial bacteria in the vagina of a mouse, reduce the colonization of harmful bacteria and show characteristics similar to prebiotics. Therefore, the medicinal and edible prebiotic simulant, namely the agkistrodon halys, can be independently used or a mixture prepared from the agkistrodon halys and other substances can be used for preparing medicines or preparations for promoting the growth of probiotics, enriching beneficial bacteria in vagina and inhibiting harmful bacteria in vagina.

The invention discloses a snake read wine capable of inhibiting cancer cell growth and a preparation method thereof, the snake read wine is prepared by taking white spirit as a main material, adding an agkistrodon halys extract and a zaocys dhumnade extract as auxiliary materials, and performing pretreatment, soaking, clarification, filtration, blending, storage and packaging. The method is simple and suitable for industrialization and large-scale production, zaocys dhumnade and agkistrodon halys are used as main raw materials, the effect of inhibiting various cancer cells is achieved, and good cancer prevention and resisting effects are achieved after long-term drinking.

The invention discloses a traditional Chinese medicine composition for treating scleroderma, which is prepared from the following traditional Chinese medicines in parts by weight: 60-100 parts of agkistrodon halys, 60-100 parts of cobra and 30-50 parts of dragon's blood. In the formula, the agkistrodon halys and the cobra have the effects of dispelling wind, dredging collaterals and resisting rheumatism, the dragon's blood has the effects of removing blood stasis and relieving pain, the formula conforms to the treatment principle of scleroderma for promoting blood circulation, removing blood stasis, dispelling wind and dredging collaterals, and research in recent years shows that the snake medicine still has the effect of regulating the immune function. Clinical experiment research shows that the treatment effect of the Soukang capsule taken by a scleroderma patient is compared with the treatment effect of a patient taking penicillamine in the same period, and the effectiveness and the safety of the Soukang capsule in treating systemic scleroderma are proved, so that the medicine disclosed by the invention has a remarkable effect of treating the scleroderma and has a good application value.