74 results about "Agkistrodon halys" patented technology

The invention discloses a method for refining a polyvalent ASV (anti-snake venom) lyophilized serum, pertaining to an immune serum containing antibody which aims at least two kinds of antigens. The serum takes the venom of Ancistrodon acutus, Agkistrodon halys, Pit-vipers, bamboo snakes, adders and the like as the immune antigen which is inoculated into the bodies of animals after degerming and dialysis, and then titer immune serum after purification is used for collecting the antibody which is then desalted and lyophilized; mice are used for evaluating the LD50 of the virulence of the venom and the titer of the ASV serum; the antibody inoculated into the bodies of the animals is one of the venom, toxoid or snake venom or the composition thereof, and glutaraldehyde or formaldehyde is used for toxin detoxication; before immunity, anti-tetanic toxoid condensed antibody for human beings is used for carrying out minim gradual immunization on the animals, or an Emuade adjuvant is used for immunization purpose. The polyvalent ASV lyophilized serum complies with the biological product standards upon the tests and inspections of purity and specific activity, safety, pyrogen, hypersusceptibility, dissolution and the like; moreover, the serum can cure sufferers who are bitten by vipers with the advantages of high purity, low foreign protein, easy preservation and quick dissolving capacity.

The invention provides a formula and a preparation method of a Chinese medicine health-care wine and belongs to a formula and a preparation method of a wine. The health-care wine comprises the following raw materials in parts by weight: 5-25 parts of cordyceps militaris or cordyceps sinensis, 10-30 parts of sealwort, 10-30 parts of kudzuvine root, 10-30 parts of barbary wolfberry fruit, 10-20 parts of poria cocos, 10-25 parts of hawthorn and 5-20 parts of agkistrodon halys. The preparation method of the health-care wine comprises the steps of 1, adding purified water or deionized water into raw edible white spirit and mixing into a wine base to serve as an extraction solvent; 2, mixing 10-30 parts of sealwort with 10-30 parts of barbary wolfberry fruit, moistening by using the wine base and smashing into fine particles; 3, smashing the cordyceps militaris, the kudzuvine root, the poria cocos, the hawthorn and the agkistrodon halys into coarse powders; 4, baking the agkistrodon halys powder in a baking oven; and 5, extracting by adopting a repercolation method, namely, mixing the smashed and processed raw materials according to the formula ratio, adding the water base, uniformly mixing and sealing, uniformly putting the moistened raw materials into a percolation tank, collecting the percolate according to the ratio of collecting 4L percolate each kilogram of the raw materials and clarifying and filtering to obtain the health-care wine. The health-care wine has the efficacies of benefiting qi, tonifying spleen, nourishing liver and kidney, promoting blood circulation, dredging collaterals, dissolving turbidity and lowering lipid.

The present invention provides a method for the treatment and / or prevention of a bacterial related condition comprising administering to a subject a therapeutically effective amount of at least one phospholipase, isoform, derivative, mutant and / or fragment thereof. The phospholipase, isoform, derivative, mutant and / or fragment thereof, may be obtained from at least one venom selected from the group consisting of: Daboia russelli russelli, Daboia russelli siamensis, Daboia russelli pulchella, Crotalus adamanteus, Crotalus durissus terrificus, Pseudechis australis, Agkistrodon halys, Pseudechis guttata, Bitis arietans, Bitis gabonica rhinoceros, Echis carinatus, Acanthopis antarticus, Bungarus candidus, Bothrops asper, Bothrops jararacussu and / or Apis mellifera. The present invention also provides isolated peptides comprising at least one amino acid sequence selected from the group consisting of: SEQ ID NO:1, SEQ ID NO:2, an isoform, derivative, mutant and / or fragment thereof.

The present invention provides a wheat germ cell-free protein synthesis system for high level expression of recombination snake venom kininogenase. According to the system, snake venom kininogenase gene of agkistrodon halys pallas is cloned through reverse transcription, wheat germ cell-free expression plasmid pCS<2+> / VK is constructed, a wheat germ extract is prepared, an in vitro transcription and translation system is established, the snake venom kininogenase is expressed and the system is optimized. The N terminal of the exogenous protein expressed by the expression system has the His protein tag, and a Ni column elution method is adopted to carry out purification, such that the subsequent purification of the recombinant protein is substantially simplified, the purification efficiency is high, and the cost is low. The exogenous protein further has biological activity, and provides the foundation for the next clinical study.

The invention discloses a method for refining a polyvalent ASV (anti-snake venom) lyophilized serum, pertaining to an immune serum containing antibody which aims at least two kinds of antigens. The serum takes the venom of Ancistrodon acutus, Agkistrodon halys, Pit-vipers, bamboo snakes, adders and the like as the immune antigen which is inoculated into the bodies of animals after degerming and dialysis, and then titer immune serum after purification is used for collecting the antibody which is then desalted and lyophilized; mice are used for evaluating the LD50 of the virulence of the venom and the titer of the ASV serum; the antibody inoculated into the bodies of the animals is one of the venom, toxoid or snake venom or the composition thereof, and glutaraldehyde or formaldehyde is used for toxin detoxication; before immunity, anti-tetanic toxoid condensed antibody for human beings is used for carrying out minim gradual immunization on the animals, or an Emuade adjuvant is used for immunization purpose. The polyvalent ASV lyophilized serum complies with the biological product standards upon the tests and inspections of purity and specific activity, safety, pyrogen, hypersusceptibility, dissolution and the like; moreover, the serum can cure sufferers who are bitten by vipers with the advantages of high purity, low foreign protein, easy preservation and quick dissolving capacity.

The invention discloses a Chinese medicinal preparation for promoting qi circulation, promoting blood circulation, softening hard mass and eliminating stagnation for people who have qi-depression and blood-stagnation physique, a preparation method thereof and application thereof. The Chinese medicinal preparation comprises the following raw material medicaments in part by mass: 20 to 60 parts of peach seed, 30 to 50 parts of semen sinapis albae, 40 to 70 parts of kelp, 20 to 70 parts of oyster shell, 30 to 80 parts of mongolian dandelion herb, 40 to 60 parts of malt, 30 to 70 parts of liquoric root, 30 to 70 parts of chicken's gizzard-membrane, 10 to 40 parts of seville orange flower, 10 to 40 parts of reddish orange, 20 to 60 parts of tangerine pith, 30 to 80 parts of tangerine seed, 40 to 80 parts of folium citri reticulatae, 30 to 70 parts of medlar, 50 to 80 parts of asparagus, 20 to 70 parts of lucid ganoderma, 40 to 70 parts of agkistrodon halys and 50 to 80 parts of citrus chirocarpus. The Chinese medicinal preparation for promoting qi circulation, promoting blood circulation, softening hard mass and eliminating stagnation for the people with the qi-depression and blood-stagnation physique is subjected to compatibility by plants which are homologous in medicines and food, does not have side effect, and can relieve or eliminate bad symptoms such as proliferation of mammary glands, swollen breasts, breast distention before menstruation and the like of the people with the qi-depression and blood-stagnation physique.

The invention discloses a method for extracting thrombin-like enzyme from Agkistrodon halys ussuriensis snake venom, which comprises the following steps of: soaking, dissolving and centrifuging snake venom; performing SP Sepharose Fast Flow cation exchange chromatography on supernate; dialyzing eluent containing active ingredients; and performing M-Aminothenyltrotionic Acid-Agarose affinity chromatography on the dialysis sample to obtain the thrombin-like enzyme with a single ingredient. The method can be operated at normal temperature, and has the characteristics of stable process, suitability for mass production, simple operation, short production period, high product purity and the like.

The invention relates to a production method of a snake peptide wine, which is characterized by adopting the technical scheme comprising the following steps of: 1, preparing an enzymatic hydrolysate for later use by selecting living bodies of adult and healthy garter snakes and agkistrodon halys as raw materials through the processes including butchering, removing heads, tails, skins and internal organs, cleaning, mincing, slurrying, denaturing proteins, adjusting concentration and temperature, carrying out enzymatic hydrolysis, killing enzyme, carrying out centrifugal filtration, carrying out ultrafiltration membrane filtration, concentrating and the like; 2, preparing a Chinese medicine liquid for later use by selecting red ginseng, radix astragali, angelica sinensis, atractylodes macrocephala, ligusticum wallichii, medlar, tuckahoe, radix rehmanniae preparata, cinnamon, radix glycyrrhizae and the like as raw materials through the processes including blending, cleaning, removing moisture, adding white spirit for soaking, filtering, and the like; and 3, adding auxiliary materials into the liquids obtained in the steps 1 and 2, mixing, adjusting the concentration, split-charging, checking and packaging to obtain a finished product.

The invention relates to a method for producing snakeskin peptide capsules. The method disclosed by the invention is characterized by adopting the following technical scheme: 1, carrying out slaughtering, head and tail removal, skin taking, cleaning, mincing, pulping, protein denaturation, concentration adjustment, temperature adjustment, enzymolysis, enzyme inactivation, centrifugal filtration, ultrafiltration membrane filtration, microwave vacuum drying and the like on raw materials, wherein adult healthy living Zaocys dhumnades and agkistrodon halys bodies as raw materials, thereby obtaining dry snakeskin peptide powder for standby; 2, carrying out mixing, cleaning, soaking, frying, filtering, microwave vacuum drying and the like on the raw materials, wherein angelica, roots of hairy asiabell, jujube, yam, medlar, tuckahoe, kudzuvine roots, lotus seeds, Gordon euryale seeds, licorice roots and the like are selected as the raw materials, thereby obtaining dry traditional Chinese medicine extract powder for standby; and 3, mixing the dry powder obtained in the step 1 and 2 in a ratio of (1:1)-(1:3), filling the obtained mixture into capsules (hard capsules or soft capsules), and carrying out sorting, split charging, sterilization, inspection and packaging on the obtained products, thereby obtaining finished products. The molecular weight of 60-90% of dry snakeskin peptide powder prepared according to the technical scheme is in the range of 200-5000 Dalton. The snakeskin peptide capsule manufactured according to the invention is health-care food with a strong health care function.

The invention discloses a medicament for treating cerebral infarction, which is prepared from agkistrodon halys, red ginseng, tall gastrodia tuber, astragalus, Chinese angelica, root of red-rooted salvia, earthworm, szechuan lovage rhizome and leech serving as raw materials. The medicament can be prepared into various formulations applied clinically. In the prescription of the invention, the medicament has the advantages of collaboration among monarch, minister, assistant and conductant medicaments, diagnosis and treatment based on overall analysis of the illness and the patient condition, effect of activating blood and reinforcing qi, good curative effect on cardiovascular and cerebrovascular diseases such as the cerebral infarction and the like, no toxic or side effects and safe and reliable clinical application.

The invention provides an agkistrodon halys venom thrombin with a single component and a preparation method and application thereof. The thrombin is the protein of (a) or (b), wherein (a) is the protein consisting of amino acid sequence displayed by SEQ ID NO:1, and (b) is the protein which is derived from (a), provided with agkistrodon halys venom thrombin activity and formed by replacing, deleting or adding one or a plurality of amino acids in the amino acid sequence of (a). The agkistrodon halys venom thrombin is clinically used for preventing bleeding and stopping bleeding. In the invention, the agkistrodon halys venom thrombin is obtained from agkistrodon halys venom via ion-exchange chromatography, affinity chromatography and gel chromatography. The invention also provides a drug composite containing the agkistrodon halys venom thrombin.

The present invention provides one kind of Chinese medicine composition, and its suppository and application in preparing opioid drug-relieving medicine and, especially, opioid analgesic. The Chinese medicine composition contains the main active components including tea, cassia twig, borneol and freeze dried snake venom of Agkistrodon halys Pallas. The Chinese medicine composition and its suppository is superior to commonly used Western non-opium excitant, and has fast detoxification, high safety, no toxic side effect and no addiction, and is suitable for use in household.

The invention provides traditional Chinese medicinal liquor for treating rheumatoid. The medicinal liquor is prepared from the following raw medicinal materials in parts by weight: 15 parts of Chinese angelica, 12 parts of ligusticum wallichii, 18 parts of pawpaw, 6 parts of tripterygium glycosides, 18 parts of sappanwood, 15 parts of radix clematidis, 15 parts of safflower, 10 parts of agkistrodon halys and 6 parts of dinodon rufozonatum, has the functions of relieving pain and syndromes, and has an effect of quickly treating rheumatoid.

The present invention discloses an agkistrodon halys-papaya solid beverage, and particularly relates to an agkistrodon halys-papaya solid beverage adopting Chinese herbs such as agkistrodon halys and papaya as main raw materials. In the prior art, the taking way of the commonly used Chinese herbs requires long-time boiling so as to waste the time, and application ranges of tablets, granules, capsules and the like produced through the extraction process and adopting the traditional Chinese medicine extract as the main component are narrow. A purpose of the present invention is to solve the technical problems in the prior art. The agkistrodon halys-papaya solid beverage is prepared from the following raw materials by weight: 90-110 parts of agkistrodon halys, 90-110 parts of zoacys dhumnades, 700-900 parts of papaya, 1400-1600 parts of radix puerariae, 500-700 parts of cinnamomum cassia, and 280-320 parts of alpinia officinarum hance. With the technical scheme, the vacuum concentration, centrifugation separation and other modern processes are adopted, the product quality is comprehensively improved from the nutrition, the taste and the absorption, and the effects of wind-evil dispelling, collateral dredging and spasm stopping are provided.

The invention provides a genetic engineering bacterium expressing recombinant snake venom kininogenase efficiently. A snake venom kininogenase gene in Agkistrodon halys pallas is cloned through reverse transcription, a pronucleus recombinant expression plasmid pET30a / VK (venom kininogenase) is constructed and then is transferred into escherichia coli, and the strains which can secrete and express efficiently are screened out. A terminal N of foreign protein expressed by the expression system is provided with an His protein tag, purification is carried out by adopting a Ni column eluting method, follow-up purification operation of recombinant protein is greatly simplified, purity of the obtained recombinant protein reaches up to more than 90%, and purification efficiency is high and cost is low, thus the genetic engineering bacterium disclosed by the invention is applicable to large-scale preparation of protein.

The invention relates to the technical field of chemical analysis and quantitative detection, in particular to a quantitative detection method of venom thrombin-like enzyme. The quantitative detectionmethod of the venom thrombin-like enzyme comprises the following steps: taking an agkistrodon halys venom thrombin-like enzyme characteristic polypeptide reference substance with an amino acid sequence of LDSPVSNSAHPLSLPSSAPSVGSVCR to prepare a series of concentration reference substance solutions; respectively adding the test solution, carrying out enzymolysis, and taking the supernatant after enzymolysis as a series of solutions to be detected; and respectively injecting a to-be-detected solution into a liquid chromatography-mass spectrometer, and selecting a qualitative ion pair and a quantitative ion pair to detect the content of the characteristic polypeptide in the to-be-detected solution. According to the method, the content of the thrombin-like enzyme in the agkistrodon halys venom and the extract thereof is determined by adopting a standard addition method, the method is simple, convenient and rapid, the matrix interference is less, the sensitivity is high, the quantificationis accurate, the blank of the quality standard of the agkistrodon halys venom and the extract thereof is filled, and the quality control level is improved.

The invention relates to a poisonous snakes drug and its production method. The method comprises: soaking Deinagkistrodon acutus, Naja, Bungarus multicinctus, Bungarus fasciatus, Agkistrodon halys, Trimeresurus stejnegeri, Zaocys dhumnades and the like in baijiu. A production of a drug of various poisonous snakes comprises: soaking the poisonous snakes above in Baijiu for 2-4 months, including, in proportion with respect to the baijiu, Deinagkistrodon acutus 700 g, Naja 500 g, Bungarus multicinctus 500 g, Bungarus fasciatus 400 g, Agkistrodon halys 100 g, Trimeresurus stejnegeri 100 g, Zaocys dhumnades 500 g, and baijiu 10000 g. The drug is simple to make and has good efficacy, with curative rate up to 99%. The drug is also capable of treating skin diseases and cancers and resisting viruses. The drug has no special requirements on storage environment and treatment environment and is simple to use and convenient to carry.

The invention discloses a plaster for treating neck-shoulder and lumbocrural pain and a preparation method of the plaster. The plaster consists of the following components: flos carthami, radix aconiti kusenzoffii, radix aconiti, radix achyranthis bidentatae, lycopodium clavatum, tuberculate speranskia herb, sappan wood, eucommia bark, rhizoma drynariae, radix dipsaci from Sichuan of China, borax, semen strychni, cornu cervi, obscured homalomena rhizome, dadix veratri, clove, radix angelicae, herba piperis puberuli, herba asari, cassia bark, rhizoma arisaematis, unprocessed rhizoma pinelliae, rhizoma ligustici wallichii, rhizoma atractylodis, raw pangolin scales, ground beetles, cuttle bone, radix fici simplicissimae, Japanese onion herb, rhizoma of aconitum kongboense lauener, cassia bark, rhizoma seu radix notopterygii, radix angelicae pubescentis, mylabris radix asparag, honeysuckle flowers, caulis sinomenii, darura stramonium linn, panax notoginseng, cortex albiziae, fructus cnidii, chrysanthemum morofolium ramat, agkistrodon halys, roasted rhizoma atractylodis macrocephalae, root of samp soniihance, paniculate swallowwort root, radix clematidis, rhizoma corydalis, long-noded pit viper, zaocys dhumnade, sambucus williamsii hance, anise, red psychotria branchlet and leaf, parthenocissus tricuspidata, scorpio, centipede, dragon's blood, frankincense, myrrh, musk, dried ginger, gum tragacanth, beeswax and honey. The preparation method comprises the following steps: selecting, cleaning and soaking materials; and decocting the materials, and spreading the materials so as to obtain the plaster. The plaster provided by the invention has beneficial effects that the plaster is practical to use and the plaster is prepared from pure natural Chinese herbal medicines.

The invention relates to the technical field of traditional Chinese medicine health food, in particular to jujube with the same source of medicine and food for treating lung cancer and a preparation method thereof. The jujube is made by fumigation of traditional Chinese medicine decoction, and the decoction includes the following raw materials in parts by weight: 300-400 parts of fresh aloe leaves, 250-350 parts of cactus, 10-20 parts of dried ginseng, 10-15 parts of salvia miltiorrhiza, 5 parts by weight. ~10 parts of Ganoderma lucidum, 0.2~0.5 parts of Cordyceps sinensis, 20~30 parts of tea polyphenols, 5~8 parts of Baiji, 10~13 parts of Chuan Fritillaria, 6~9 parts of Digupi, 8~13 parts of Flat Fritillaria, 20-25 parts Panax notoginseng, 6-9 parts Polygalaceae, 3-5 parts Ginkgo biloba, 2-5 parts Eupatorium, 3-6 parts Agkistrodon, 6-12 parts Pueraria, 10-18 parts Scallion, 2-6 parts Polygonum multiflorum, 3-7 parts of Morus alba, 2-8 parts of Poria cocos, 10-15 parts of rhodiola, 2-6 parts of gecko, 10-26 parts of angelica. The invention has high cure rate and no side effect.

The invention relates to the technical field of chemical analysis and quantitative detection, in particular to an agkistrodon halys venom phospholipase A2 marker polypeptide and application thereof. The amino acid sequence of the characteristic polypeptide is WDDYTYSWK, the characteristic polypeptide can be used for detecting the content of the agkistrodon halys venom phospholipase A2 in a sample to be detected, and the detection method comprises the following steps: taking the characteristic polypeptide of the agkistrodon halys venom phospholipase A2 with the amino acid sequence being WDDYTYSWK, and preparing a series of concentration reference substance solutions; taking a sample to be detected, performing enzymolysis, and taking supernate after enzymolysis as a test solution; and respectively injecting into a liquid chromatography-mass spectrometer, and selecting double charges 632.4-624.0 as quantitative ions to detect the content of the characteristic polypeptide of the agkistrodon halys venom phospholipase A2 in a sample to be detected, so that the quality control level of an agkistrodon halys venom medicine can be greatly improved, and the method has a very good guiding effect on the quality control of the agkistrodon halys venom, an intermediate and a medicinal preparation thereof.

The present invention belongs to the technical field of food and health-care product processing, and more particularly to a fatigue preventing and body immunity and resistance activating health-care functional food and a preparation method thereof. Agkistrodon halys, zaocys dhumnades, sea cucumbers, soybeans, red dates, ginger powder and chicken meat are mixed, then water is added to boiled the mixture, and the obtained mixed soup material is subjected to filtration, packaging and sterilization to obtain the finished products. The nutritional value of the snakes are preserved to play the nutritional advantages that the snakes are full of treasures. By advanced filling and high-temperature process, the snake soup is processed into high-quality functional food which is nutritious, convenient, and has efficacies of caring health and tonifying body. The prepared health-care functional food needs no secondary processing, can be eaten once being heated, is free of any preservatives, has relative food mouthfeel and comprehensive and balanced nutrition, fully reflects the medicinal values of snake skin, snake meat, snake fat and snake galls, contains a variety of biologically active ingredients, and has very good efficacies of nourishing yin and supplementing kidney, improving sleep and preventing fatigue, and enhancing body immunity and resistance.

The inventon discloses a kidney-reinforcing and yang-tonifying healthcare snake wine. The components for preparing the wine are as follows: twelve Chinese medicines of agkistrodon halys, astragalus membranaceus, cynomorium songaricum, longspur epimedium, morinda officinalis, 180-220g of sweet cistanche, lycium barbarum, semen cuscutae, angelica sinensis, fructus psoraleae, cinnamon and Chinese yam are added into every one hundred jin of white wine, the agkistrodon halys is steamed and is placed with other Chinese medicines into a wine jar for being soaked for 1 to 3 years, and then the mixture is taken out and filtered to obtain a supernate. The snake wine prepared according to the invention has the effects of benefiting qi for activating blood circulation and reinforcing the kidney and tonifying yang, and a good health effect is realized for elder people and people with insufficiency of kidney-yang and sexual dysfunction when the wine is drunk in a long term.

The invention provides a composition for treating gout. The composition is prepared from the following raw materials in parts by weight of 45-55 parts of honeysuckle flowers, 25-35 parts of dandelions, 10-20 parts of glossy ganoderma, 2-8 parts of rhizoma gastrodiae, 2-8 parts of American ginseng, 2-8 parts of agkistrodon halys, 10-20 parts of chrysanthemums, 10-20 parts of raspberries and 10-20 parts of mulberries. According to the composition for treating gout provided by the invention, the raw material components have the effect of synergy, and the raw materials are from natural animal andplant sources, so that the composition is mild in action and free from toxic and side effects, and can be taken for a long term. The composition is notable in treatment effects, and is high in effective rate. The composition can be used for treating both manifestation and root cause of a disease, and has double effects of prevention and treatment.

The invention discloses a traditional Chinese medicine formula for treating lumbar and cervical spondylosis. According to the formula, the traditional Chinese medicine is prepared from the following components in parts by weight: 20 to 25 parts of fructus lycii, 10 to 15 parts of agkistrodon halys, 8 to 15 parts of rhizoma polygonati, 20 to 30 parts of rhizoma dioscoreae, 10 to 15 parts of fructus mori, 6 to 10 parts of cortex cinnamomi, and 10 to 20 parts of poria cocos. The optimal weight part ratios of all medicines in the formula are as follows: 24 parts of fructus lycii, 12 parts of agkistrodon halys,12 parts of rhizoma polygonati, 24 parts of rhizoma dioscoreae, 12 parts of fructus mori, 8 parts of cortex cinnamomi, and 15 parts of poria cocos. The traditional Chinese medicine formula disclosed by the invention is particularly suitable for patients suffering from insufficiency of kidney-qi and obstruction of governor vessels; the patients basically have no symptoms after resting in bed, and the patients feel comfortable in the morning, soreness, numbness and distending pain occurs only after noon or activity exhaustion; therefore, once the medicine is orally taken, the medicine takes effect quickly and has a significant effect.

The present invention relates to the technical field of chemical analysis and quantitative detection, in particular to a quantitative detection method for snake venom thrombin-like enzyme (SVTLE) from Agkistrodon halys pallas. The quantitative detection method for the SVTLE includes the following steps of taking a reference substance of marker peptide for the SVTLE from Agkistrodon halys pallas with an amino acid sequence of LDSPVSNSAHIAPLSLPSSAPSVGSVCR, and preparing a series of reference solutions with different concentrations; adding the reference solutions in test solutions respectively for enzymolysis, and then taking a supernatant after enzymolysis as a series of solutions to be detected; and adding the solutions to be detected in a liquid chromatogram-mass spectrometer, and then selecting a qualitative ion pair and a quantitative ion pair to detect contents of marker peptide in the solutions to be detected.

The invention discloses a method for identifying agkistrodon halys venom by applying mass spectrometry. The method comprises the following steps of: (1) dissolving agkistrodon halys venom to prepare aprotein solution; (2) carrying out SDS-PAGE analysis; and (3) carrying out LC-MS / MS mass spectrometry to carry out agkistrodon halys venom component identification. In addition, the invention furtherdiscloses application of the identification method in the preparation of products for identifying the agkistrodon halys venom and application in the preparation of agkistrodon halys venom resistant serum for treating agkistrodon halys venom poisoning. According to the invention, the agkistrodon halys venom can be identified in a laboratory; agkistrodon halys venom is more accurately subjected toquality control, so that specific drug anti-agkistrodon halys poison serum for treating agkistrodon halys venom poisoning is produced; and the method is very important for ensuring the safety, effectiveness and quality controllability of anti-agkistrodon halys venom serum products and is of great significance for reference of the diagnosis and legal medical expert identification of the types of venom caused by snake venom poisoning. The method has the advantages of high detection sensitivity, high correct detection rate, no cross reaction and simple operation process.

The invention relates to the field of traditional Chinese medicines, particularly to a traditional Chinese medicine formula for treating rheumatism and rheumatoid arthritis. Aiming at the modern western medicine which mainly adopts symptomatic treatment with non-ideal effect, the invention aims to provide the traditional Chinese medicine formula for treating rheumatism and rheumatoid arthritis, so as to effectively overcome defects in the prior art. The technical scheme for solving the problem is that the traditional Chinese medicine formula for treating rheumatism and rheumatoid arthritis is prepared from the following medicines: 150-200 g of zaocys dhumnade, 75-100 g of sealwort, 100-150 g of papaya, 125-175 g of coix seed, 90-120 g of arillus longan and 120-150 g of agkistrodon halys. According to the traditional Chinese medicine formula, Chinese medicine theories are utilized as guidance, and the purposes of treating rheumatism, rheumatoid arthritis, and neck-shoulder and lumbocrural pain are achieved due to scientific medicine compatibility. The traditional Chinese medicine formula is scientific in compatibility, reasonable in dosage, easy to produce, low in cost, convenient to use and good in curative effect, and is innovation for treating rheumatism, rheumatoid arthritis, and neck-shoulder and lumbocrural pain.

The invention relates to an ablation and embolism extinguishing preparation of Chinese medicine which is designed aiming at the technical problems existing in the prior art that a plurality of medicaments have long taking time, large dosage, are slow in taking effect and do not have ideal clinical efficacy. The components of the preparation is as follows according to weight percentage: 10 to 30g of rhizoma gastrodiae, 50 to 70g of radix salviae miltiorrhizae, 60 to 80g of astragalus root, 20 to 40g of red peony root, 10 to 30g of dlbergia wood, 30 to 50g of turmeric, 20 to 30g of agkistrodon halys, 20 to 30g of earthworm and 30 to 50g of szechwan lovage rhizome. The preparation method comprises the steps of: putting the pure Chinese medicines of rhizoma gastrodiae, salviae miltiorrhizae, astragalus root, red peony root, dlbergia wood, turmeric and szechwan lovage rhizome in a container after classifying and purifying; drying the agkistrodon halys and earthworm by mild fire, and then preparing into grains and packing into capsule after being ground into powder, screened on 100 mesh sieve and being fully mixed and stirred with all the purified Chinese medicine; and irradiating conventionally by medical Co for later use. The preparation has the characteristics and beneficial effects that: after years of clinical observation, the preparation has reliable embolism resisting and ablation effects, effectively reduces the blood plasma and the whole blood contrast viscosity and reduces the cohesiveness of blood platelet, effectively recovers sequela caused by cardiovascular and cerebrovascular diseases, and especially has the double effects of stroke rehabilitation and preventing from second attack.

The body lotion of Agkistrodon halys tissue extract of the present invention belongs to the technical field of preparation of cosmetics production enterprises. The body lotion made of traditional Chinese medicines such as chrysanthemum, woody, gentian, chrysanthemum, magnolia, and natural mineral medicine medical stone, tourmaline and other nanotechnology, can be applied, absorbed, and penetrated deep into the subcutaneous area through the skin 3— 5mm, automatically heats up, activates, organizes, promotes metabolism, automatically and continuously generates negative ion far infrared, effectively promotes skin blood circulation, prevents various skin diseases and skin aging and aging, skin care and beauty.