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Method for preparing hericium erinaceus oligosaccharide on basis of film separation technology

A technology of Hericium erinaceus and oligosaccharide, which is applied in the field of efficient preparation of Hericium erinaceus oligosaccharides based on membrane separation technology, can solve many problems such as the research on the preparation process of oligosaccharides, and achieves low cost, short production cycle and high purity. high effect

Inactive Publication Date: 2019-03-08
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] At present, there are many studies on the preparation process of oligosaccharides.

Method used

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  • Method for preparing hericium erinaceus oligosaccharide on basis of film separation technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Add 100g of Hericium erinaceus powder into distilled water according to the mass ratio of material to liquid 1:20, extract at 70°C for 2h, filter with suction, repeat the extraction of the filter residue once, combine the filtrate obtained from the two extractions, and concentrate under reduced pressure to 1 / 4 of the original volume to obtain a concentrated solution, mix the obtained concentrated solution with 90% ethanol aqueous solution at a volume ratio of 1:3 for alcohol precipitation, and collect the supernatant;

[0042] (2) Place the supernatant of step (1) gained in the feed liquid tank of the high-pressure flat membrane machine, then put the PES ultrafiltration membrane of 1000Da in the high-pressure flat membrane machine, seal the oxygen barrier, at pressure 1MPa, rotating speed 600r Ultrafiltration at 1 / min, when the raw material liquid is concentrated to 1 / 4 of the original volume after ultrafiltration, add water to dilute 1 times and repeat the ultrafilt...

Embodiment 2

[0046] (1) Add 100 g of Hericium erinaceus powder into distilled water according to the mass ratio of material to liquid at 1:20, extract at 60°C for 3 hours, filter with suction, repeat the extraction of the filter residue once, combine the filtrate obtained from the two extractions, and concentrate under reduced pressure to 1 / 4 of the original volume to obtain a concentrated solution, mix the obtained concentrated solution with an ethanol aqueous solution with a volume fraction of 80% at a volume ratio of 1:3 for alcohol precipitation, and collect the supernatant;

[0047] (2) Place the supernatant of step (1) gained in the feed liquid tank of the high-pressure flat membrane machine, then put the PA ultrafiltration membrane of 1000Da in the high-pressure flat membrane machine, seal the oxygen barrier, at pressure 1MPa, rotating speed 600r Ultrafiltration at 1 / min, when the raw material liquid is concentrated to 1 / 4 of the original volume after ultrafiltration, add water to di...

Embodiment 3

[0051] (1) Add 100 g of Hericium erinaceus powder into distilled water according to the mass ratio of material to liquid at 1:20, extract at 80°C for 1 hour, filter with suction, repeat the extraction of the filter residue once, combine the filtrate obtained from the two extractions, and concentrate under reduced pressure to 1 / 4 of the original volume to obtain a concentrated solution, mix the obtained concentrated solution with an ethanol aqueous solution with a volume fraction of 95% at a volume ratio of 1:3 for alcohol precipitation, and collect the supernatant;

[0052] (2) Place the supernatant of step (1) gained in the feed liquid tank of the high-pressure flat membrane machine, then put the PES ultrafiltration membrane of 2000Da in the high-pressure flat membrane machine, seal the oxygen barrier, at pressure 1MPa, rotating speed 600r Ultrafiltration at 1 / min, when the raw material liquid is concentrated to 1 / 4 of the original volume after ultrafiltration, add water to di...

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Abstract

The invention provides a method for preparing hericium erinaceus oligosaccharide on the basis of a film separation technology. The molecular weight of the obtained oligosaccharide can be regulated andcontrolled, the hericium erinaceus oligosaccharide is mainly micromolecular hericium erinaceus oligosaccharide of which the molecular weight is 300-2000 Da, ultrafiltration and nanofiltration technologies are applied to separation of oligosaccharide of edible mushrooms, a purpose of efficiently purifying the oligosaccharide can be achieved, thus, the purity of the finally obtained oligosaccharideis high, the quality is good, the time consumed in the preparation process is short, the efficiency is high, chemical pollution is avoided, and the energy consumption is low.

Description

(1) Technical field [0001] The invention relates to a method for preparing edible fungus oligosaccharides, in particular to a method for efficiently preparing Hericium erinaceus oligosaccharides based on membrane separation technology. (2) Background technology [0002] Oligosaccharides, also known as oligosaccharides, generally refer to polymers composed of 2-10 monosaccharides. Due to the different positions and types of monosaccharides, a wide variety of oligosaccharides are formed. From the known common sucrose, maltose , lactose, etc., to xylooligosaccharides, soybean oligosaccharides, chitooligosaccharides, etc., are all important functional substances in organisms. Studies have shown that oligosaccharides have a good effect on improving lipids, lowering cholesterol, lowering blood sugar, promoting the proliferation of bifidobacteria in the intestine, and improving the structure of the flora. Edible fungi with delicious meat and rich nutrition contain a lot of polysac...

Claims

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Application Information

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IPC IPC(8): C07H1/08C07H3/04C07H3/06C08B37/00
CPCC07H1/08C07H3/04C07H3/06C08B37/0003Y02P20/10
Inventor 蔡铭孙培龙陈思杨开
Owner ZHEJIANG UNIV OF TECH
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