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A lateral flow chromatography test paper and its preparation method and application

A lateral flow chromatography and test strip technology, applied in biological testing, analytical materials, instruments, etc., can solve problems such as inability to mass production, large environmental impact, and complex preparation, and achieve mass production, low environmental impact, and biological good compatibility

Active Publication Date: 2022-01-28
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above method has the disadvantages of complex preparation, cumbersome operation, high cost, great influence on the environment, and inability to produce in batches. It is still not suitable for improving the sensitivity of lateral flow chromatography test paper, and cannot be widely used in resource-poor areas.

Method used

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  • A lateral flow chromatography test paper and its preparation method and application
  • A lateral flow chromatography test paper and its preparation method and application
  • A lateral flow chromatography test paper and its preparation method and application

Examples

Experimental program
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Effect test

preparation example Construction

[0034] (3) Preparation of lateral flow test paper: lateral flow chromatography test paper includes sample pad, binding pad 2, nitrocellulose membrane 3, absorbent paper 4, paper 8 embedded with hydrophobic cotton thread and adhesive backing plate 5; sample pad 1, binding pad The pad 2, the paper 8 embedded with hydrophobic cotton thread, the nitrocellulose membrane 3 and the absorbent paper 4 are pasted on the adhesive backing board 5 with overlapping 2 mm in order.

[0035] Preparation of reagents: sodium citrate and chloroauric acid were used to prepare gold nanoparticles, and gold nanoparticles were modified with detection probes, and control probes and capture probes were modified with streptavidin.

[0036] Detection of flow test paper: Take 0.5 μL of the modified control probe and capture probe and drop them on the nitrocellulose membrane 3 respectively, add 8 μL of the modified gold nanoparticles on the binding pad 2, and put them in an oven at 37°C. Dry in medium tempe...

Embodiment 1

[0039] (1) Cotton thread pretreatment: Take a commercially available cotton thread and place it in a solution of cetyltrimethoxysilane with a concentration of 0.025% for 30 minutes, take it out, wash it under running water, and dry it to obtain a hydrophobic cotton thread 9; Alkyltrimethoxysilane is diluted with ethanol, and an appropriate amount of acetic acid is added as a catalyst, wherein ethanol: acetic acid is about 9:1.

[0040] (2) Paper preparation: Take an appropriate amount of coniferous wood fiber and deflagrate it in a standard fiber deflattering machine with a speed of 20000r / min to disperse the fibers evenly. The 2 treated cotton threads are placed on the forming net of the paper former, poured into the pulp suspension, and dehydrated quickly to obtain a wet paper sheet, and the wet paper sheet is dried at 105°C±2°C for 3 minutes under a pressure of 0.6MPa. The basis weight of the paper is 80g / m 2 .

[0041] (3) Preparation of lateral flow test paper: lateral fl...

Embodiment 2

[0046] (1) Cotton thread pretreatment: Take 2 commercially available cotton threads and place them in a 0.1% hexadecyltrimethoxysilane solution for 30 minutes, take them out, wash them under running water, and dry them to obtain hydrophobic cotton threads 9; After the cotton thread is treated with cetyltrimethoxysilane, the hydrophobicity of the cotton thread is uniformly enhanced; the cetyltrimethoxysilane is diluted with ethanol, and an appropriate amount of acetic acid is added as a catalyst, wherein ethanol: acetic acid is about 9:1 .

[0047] (2) Paper preparation: Take an appropriate amount of coniferous wood fiber and decompose it in a standard fiber deflattering machine, the decomposing speed is 20000r / min, so that the fibers are evenly dispersed, and the 2 treated cotton threads are placed on the forming net of the paper former, poured into the pulp suspension Liquid, rapid dehydration to form wet paper sheets, dry the wet paper sheets at 150°C±2°C for 3 minutes under...

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Abstract

The invention discloses a lateral flow chromatography test paper, which comprises a sample pad, a binding pad, a nitrocellulose membrane, water-absorbing paper, a paper embedded with hydrophobic cotton threads, and an adhesive sheet; a sample pad, a bonding pad, and paper embedded with hydrophobic cotton threads , nitrocellulose membrane and water-absorbing paper are overlapped by 2mm in sequence and fixedly pasted on the backing board. The cotton thread with a certain hydrophobic property is embedded in the paper during the papermaking process, and the two are combined as a fluid channel and added to the traditional side flow. In front of the nitrocellulose membrane of the chromatography test paper, change the structure of the test strip, slow down the flow rate of the target on the nitrocellulose membrane, prolong the reaction time between the target and the probe, thereby enhancing the detection sensitivity. The cotton thread used is a common textile material in daily life, which is cheap and easy to obtain, and is made of fiber materials similar to paper; and the cotton thread and paper are combined through the traditional papermaking process, the operation is simple and fast, and it is expected to realize mass production. For HIV nucleic acid detection.

Description

technical field [0001] The invention belongs to the field of test paper preparation, and in particular relates to a method and application for enhancing the sensitivity of lateral flow test paper. Background technique [0002] With the increasing incidence of infectious diseases, food contamination and water contamination, applications such as medical diagnosis, environmental monitoring and food safety testing are becoming more and more important, and they are mainly for analytes such as nucleic acids in body fluids and other biochemical fluids , detection of proteins, drugs and metabolites, etc. The traditional method is to carry out quantitative analysis of samples in the laboratory, but it often requires large and expensive analytical instruments, and requires personnel with corresponding professional skills to operate, and the demand for samples is also large. Developing countries and underdeveloped areas, or emergency situations, or home health testing are difficult to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/531G01N33/543G01N33/52G01N33/558
CPCG01N33/525G01N33/531G01N33/54306G01N33/558
Inventor 张素风刘丽娜唐蕊华李菲徐峰
Owner SHAANXI UNIV OF SCI & TECH
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