Preparation method of thymosin

A technology of thymosin and thymus, applied in the field of preparation of thymosin, can solve the problems of high molecular weight of products, residual chemical reagents, low extraction efficiency of small molecular polypeptides, etc., and achieve the effects of high purity, controllable molecular weight and easy operation.

Inactive Publication Date: 2019-03-12
河北肽都生物科技集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems of chemical reagent residues, high product molecular weight, and low extraction efficiency of small molecule polypeptides existing in the existing technology for producing thymosin, the present invention provides a method for preparing thymosin

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  • Preparation method of thymosin

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Embodiment 1

[0028] This embodiment provides a kind of preparation method of thymosin, specifically comprises the following steps:

[0029] Step a, select 1000g of fresh porcine thymus, and use a liquid nitrogen grinder to grind it with liquid nitrogen as a raw material;

[0030] Step b, add 5000mL of purified water to the raw material, stir evenly, after ultrasonication for 20min, heat up to 35°C, add 8g of Candida lipase and 10g of Bacillus subtilis protease, keep for 40min, then add 3g of lactic acid bacteria aminopeptidase, keep The temperature is 4h, and finally the temperature is raised to 90°C, and the enzyme is kept for 15min;

[0031] Step c, filter with horizontal gong centrifuge (centrifugal speed is 3400r / min) after step c, deenzyme, filtrate is degreased and purified with three-phase centrifuge again; Use a nanofiltration machine with a nanofiltration membrane pore size of 0.18nm to desalt and concentrate, and the concentrate is further purified with an ion filter, and then f...

Embodiment 2

[0034] This embodiment provides a kind of preparation method of thymosin, specifically comprises the following steps:

[0035] Step a, select 10 kg of fresh bovine thymus, and use a liquid nitrogen grinder to grind it with liquid nitrogen as a raw material;

[0036] Step b: Add 60L of purified water to the raw material, stir evenly, after ultrasonication for 25min, heat up to 40°C, add 50g of Candida lipase, 50g of Aspergillus niger lipase and 120g of Bacillus subtilisin, keep for 35min, then add 40g For lactic acid bacteria aminopeptidase, keep the temperature for 3.5 hours, and finally raise the temperature to 93°C, and keep it for 12 minutes to kill the enzyme;

[0037] Step c, filter with horizontal gong centrifuge (centrifugal speed is 3600r / min) after step c, deenzyme, and filtrate degreases and purifies with three-phase centrifuge again; Use a nanofiltration machine with a nanofiltration membrane pore size of 0.2nm to desalt and concentrate, and the concentrate is furthe...

Embodiment 3

[0040] This embodiment provides a kind of preparation method of thymosin, specifically comprises the following steps:

[0041] Step a, select 100kg of fresh sheep thymus, and use a liquid nitrogen grinder to grind it with liquid nitrogen as a raw material;

[0042] Step b, add 400L of purified water to the raw material, stir evenly, after ultrasonication for 30min, heat up to 45°C, add 1.2kg of Aspergillus niger lipase and 1.5kg of Bacillus subtilis protease, keep for 30min, then add 0.5kg of lactic acid bacteria aminopeptidase, Keep the temperature for 3 hours, and finally raise the temperature to 95°C, and keep it for 10 minutes to inactivate the enzyme;

[0043] Step c, filter with horizontal gong centrifuge (centrifugal speed is 3800r / min) after step c, deenzyme, filtrate is degreased and purified with three-phase centrifuge again; Use a nanofiltration machine with a nanofiltration membrane pore size of 0.22nm to desalt and concentrate, and the concentrate is further puri...

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Abstract

The invention relates to the technical field of deep processing of animal thymuses, in particular to a preparation method of thymosin. According to the preparation method, fresh livestock thymuses areadopted as a main raw material, through pretreatment, enzymolysis, solid-liquid separation, degreasing purification, nanofiltration and concentration purification, a concentrated solution of the thymosin is obtained, and the concentrated solution can be freeze-dried to obtain a freeze-dried product. The preparation method is short in production cycle, low in cost and free of environment pollution, the obtained product is high in safety, high in purity, high in product activity and controllable in molecular weight, and can be widely used in the fields such as food, special medical food, healthcare products and medicines.

Description

technical field [0001] The invention relates to the technical field of deep processing of animal thymus, in particular to a preparation method of thymosin. Background technique [0002] The thymus is not only an immune organ, but also an endocrine organ. It is the source of lymphocytes, not only a place for T cell differentiation, but also B cells, which play an important immune role in the early childhood of animals. Recent studies have suggested that the adult thymus contributes to the remodeling of T cells. During antiretroviral therapy in patients with acquired immunodeficiency disease, the increased naive lymphocytes are mostly released from the thymus. The thymus can secrete thymosin (including thymosin α1 and thymosin β4) and thymopoietin. Thymosin α1 is an immune active peptide that mainly acts on the early and late stages of thymocyte maturation and can increase Th1, 2 on the surface of T cells. And the expression of Lyt-1, 2, 3 affect TH cell maturation in vitro....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/575C07K1/34A23L33/18
CPCA23L33/18C07K14/57581C12P21/06
Inventor 张晓东杨广杨子丰
Owner 河北肽都生物科技集团有限公司
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