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Epimedium sagittatum control extract and application thereof

A technology of epimedium extract and epimedium, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems that cannot reflect the whole picture of medicinal materials, it is difficult to ensure consistent quality, and cannot reflect the content of medicinal materials, and achieve stable properties , Easy to operate and easy to prepare

Active Publication Date: 2019-03-12
广州科曼生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, both chemical reference substances and traditional Chinese medicine reference materials have their limitations in the quality control of traditional Chinese medicine
First of all, the chemical components in traditional Chinese medicine are diverse, and a single or a few compounds cannot reflect the whole picture of the medicinal materials, and the existing standards often have many loopholes, and the phenomenon of shoddy goods often occurs
Traditional Chinese medicine control materials are affected by the place of origin and growth environment, so it is difficult to ensure the quality of each batch is consistent, and it can only be used for qualitative identification, and cannot reflect the content of medicinal materials

Method used

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  • Epimedium sagittatum control extract and application thereof
  • Epimedium sagittatum control extract and application thereof
  • Epimedium sagittatum control extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: Screening of Epimedium raw material medicinal material

[0033] This example provides a screening method for raw materials of Epimedium control extract (Arrow leaf) of the present invention.

[0034] Using thin-layer chromatography and high-performance liquid chromatography to analyze and screen the raw material of Epimedium (arrow leaf) with the chemical reference substance as the reference material.

[0035] Epimedium medicinal material was purchased from major medicinal material markets across the country, and was identified by Professor Xie Peishan as the dried leaf of Epimedium sagittatum (Sieb.et Zucc.) Maxim, a plant of Berberidaceae. After identification, 6 batches of Epimedium herbal medicine are Epimedium sagittatum, which meet the standard and can be used for future use.

[0036] 1 TLC

[0037] 1.1 Sample preparation

[0038]Chemical reference substance solution: Accurately weigh an appropriate amount of icariin and epimedin C chemical refere...

Embodiment 2

[0069] Embodiment 2: Preparation of Epimedium control extract

[0070] The present embodiment provides the preparation method of Epimedium reference extract (Arrow leaf) of the present invention, and its method flowchart is as follows image 3 shown.

[0071] One: extract

[0072] Select epimedium medicinal material (arrow leaf) and make it into powder, then add 50% ethanol (that is, the solid-liquid ratio is 1:25 (w / V)) of 25 times the volume of its mass, heat and reflux for extraction for 30 minutes, and then characterize at a medium speed Filter through filter paper, collect filter residue 1 and filtrate 1, extract filter residue 1 again in the same way, and combine the collected filtrate with filtrate 1 to obtain the extract, and evaporate the extract to dryness until there is no ethanol smell to obtain Epimedium Huskwort crude extract (Arrow leaf), fix Epimedium crude extract (Arrow leaf) with 1:20 macroporous resin column, absorb for 30-60min, wash with pure water unti...

Embodiment 3

[0085] The character analysis of embodiment 3 epimedium control extract (arrow leaf)

[0086] 1. Appearance state: the epimedium reference extract (arrow leaf) that embodiment 2 obtains is light gray purple powder.

[0087] 2. Moisture determination: according to the 2015 edition Chinese Pharmacopoeia Appendix IX G (reduced pressure drying method). The test result shows that the water content of the control extract of Epimedium (arrow leaf) is 0.50%.

[0088]3. Consistency test: Prepare 3 batches of epimedium reference extracts (arrow leaf) according to the method of embodiment 2, after measuring the TLC detection results for epimedin C and icariin between each batch The difference was very small, and the fluorescent spots of epimedin C and icariin were clearly displayed, and the distribution was very consistent; the contents of epimedin C and icariin were within the range of the blending standard as detected by high performance liquid chromatography. Therefore, the consiste...

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Abstract

The invention provides an epimedium sagittatum control extract. The epimedium sagittatum control extract is prepared by performing multiple extraction on epimedium sagittatum powder to obtain different batches of epimedium sagittatum extracts and then blending the different batches of epimedium sagittatum extracts. Due to the fact of being blended from the different batches of epimedium sagittatumextracts, the epimedium sagittatum control extracts of different batches can ensure consistency and meanwhile be stable uniform in characteristics and convenient to use. The epimedium sagittatum control extract is applied to quality control over epimedium sagittatum and epimedium sagittatum / epimedium sagittatum effective constituent-containing drugs or Chinese herbal preparations and can be directly put into use after being simply processed, thereby being simple in operation; especially when the epimedium sagittatum control extract is applied to multiple component content measurement, preparation of a control extract solution is simpler than that of control substance solutions, so that the epimedium sagittatum control extract can not only be applied to qualitative discrimination but alsoto semi-quantitative or even quantitative analysis of the drugs or Chines herbal preparations.

Description

technical field [0001] The invention relates to the technical field of quality control of traditional Chinese medicines, in particular to a preparation method of epimedium control extract (arrow leaf). Background technique [0002] The current quality control mode of traditional Chinese medicine is basically following the development of natural medicinal chemistry, with the idea of ​​analysis methods targeting one or several active ingredients of traditional Chinese medicine and quality standards that can be both qualitative and quantitative, referring to the quality of foreign herbal medicines. The control method draws on the model of chemical drug quality control, and establishes corresponding simple physical and chemical identification with the help of literature reports, and then develops to the quality standard of identification and content determination based on spectrum and chromatography. Every Chinese medicinal material is a complex of multiple components, which det...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/90G01N30/02
CPCG01N30/02G01N30/06G01N30/90
Inventor 郭隆钢许舜军孙帅许艺镌巫少娟赵岳锐谢培山许铮弟
Owner 广州科曼生物科技有限公司
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