Low-temperature straw degradation fungus JGDW-1, microbial agent thereof and application of fungus

A technology of JGDW-1, fungal agent, applied in the field of agricultural resources and environment, can solve the problems of low temperature, slow cellulose degradation, long fermentation cycle, etc., achieve broad application prospects, promote sustainable development, and accelerate straw returning to the field for maturity Effect

Active Publication Date: 2019-03-19
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

Medium- and high-temperature cellulose-degrading bacteria must work under certain temperature conditions, and there are certain restrictions on temperature, while low-temperature cellulose-degrading bacteria have much lower temperature requirements for life activities than medium- and high-temperature bacteria, and are especially suitable for solving winter problems in northern China. Long time, low temperature, slow cellulose degradation, and long fermentation cycle

Method used

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  • Low-temperature straw degradation fungus JGDW-1, microbial agent thereof and application of fungus
  • Low-temperature straw degradation fungus JGDW-1, microbial agent thereof and application of fungus
  • Low-temperature straw degradation fungus JGDW-1, microbial agent thereof and application of fungus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Isolation and Identification of Straw Degrading Fungal Strain JGDW-1

[0032] The degrading fungal strain JGDW-1 was screened from a straw accumulation place in Weifang City, Shandong Province. The suspension of the soil samples diluted step by step was applied to the CMC-Congo red plate by the gradient dilution coating method, and cultured at 10°C to screen the cellulose-degrading bacteria. Purify and isolate strains that produce larger transparent circles and grow faster on CMC-Congo red plates, inoculate them on rice straw powder medium with rice straw as the only carbon source, and culture at 10°C to compare the growth rates of each strain and growth conditions, and finally obtained the fungal strain JGDW-1 that can efficiently degrade rice straw under low temperature conditions.

[0033]CMC-Congo red medium: sodium carboxymethylcellulose (CMC-Na) 10.0g, (NH 4 ) 2 SO 4 2.0g, KH 2 PO 4 1.0g, MgSO 4 ·7H 2 O 0.5g, Congo red 0.2g, agar 20g, deionized ...

Embodiment 2

[0039] Example 2 Straw Degrading Bacteria JGDW-1 Inoculum Agent

[0040] Inoculate the strain JGDW-1 into an Erlenmeyer flask containing 50 mL of rice straw powder medium (same as in Example 1), culture it statically at 10°C for 7 days, add 30 mL of sterile water to the Erlenmeyer flask, and shake it on a shaker for 30 minutes , Filter the culture with gauze, which is the spore suspension. The spore suspension was inoculated in a liquid medium with an inoculation amount of 1%, cultured at 170 r / m for 7 days at 10°C, and centrifuged to remove bacteria and impurities to obtain a crude enzyme solution. Among them, liquid medium: rice straw powder 20g, (NH 4 ) 2 SO 4 1.4g, KH 2 PO 4 2g, MgSO 4 ·7H 2 O 0.3g, CaCl 2 2H 2 O 0.4g, Tryptone 1g, FeSO 4 ·7H 2 O0.0075g, MnSO 4 ·H 2 O 0.0025g, ZnSO 4 0.002g, CoCl 2 0.003g, 1000 mL deionized water, natural pH, autoclave at 121℃ for 20min. The rice straw powder is dried rice until the water content is below 1%, crushed, a...

Embodiment 3

[0046] The degradation effect of strain JGDW-1 on rice straw was studied by liquid fermentation in a triangular flask.

[0047] Each 250mL triangular flask is divided into 3.00g of rice straw shredded to a length of 2-3cm and 150mL of liquid culture medium after being pretreated with 2% NaOH solution and dried at 50°C. Inoculate 1.5mL per bottle 10 7 The spore suspension with a concentration of 1 spore / mL was mixed and placed on a constant temperature shaker at 10°C, 20°C, 30°C, 40°C at 170r / m, and the same amount of sterile water was inoculated as a blank control. Regularly observe the morphological changes of rice straw. After 7 days, wash off the bacteria on the straw, dry to constant weight, and measure its degradation rate.

[0048] The results showed that after inoculation at 10 and 20°C for 24 hours, the culture solution began to turn gray-black, and at this time, the rice straw began to soften and disintegrate and disperse. After 48 hours, it has completely turned bl...

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Abstract

The invention discloses a low-temperature straw degradation fungus JGDW-1 which is classified and named as Aureobasidium pullulans. The fungus is collected in a China General Microbiological Culture Collection Center on March 22, 2018, and the collection number of the fungus is CGMCC NO. 15390. The fungus can generate a degradation ring on CMC-congo red agar and can grow on the agar taking a cropstraw as a sole carbon source. The fungus can generate enzymes degraded by related cellulose and semi-cellulose, macromolecular substances in the crop straw are transformed into small molecular substances which can be absorbed and used by plants, agricultural waste is recycled under the condition of low temperature, and the fungus has a wide application prospect. Experiments indicate that after rice straws are inoculated with spore suspension, the rice straws are fermented and cultured by 170r / m liquid under the condition of 10 DEG C for 7 days, and the degradation rate of the rice straws is 47.3%.

Description

technical field [0001] The invention belongs to the field of agricultural resources and environment, in particular to the field of resource utilization of solid waste, and relates to a low-temperature (10-20°C) straw-degrading fungus JGDW-1 and its bacterial agent. The fungus JGDW-1 and its bacterial agent are specially used for Degrade waste crop stalks to produce organic fertilizers and realize the resource utilization of agricultural waste. Background technique [0002] my country is a large agricultural country, which generates a large amount of agricultural wastes every year, such as wheat, rice, corn and other crop straws. On the one hand, these straws contain a large amount of nutrients necessary for plant growth, such as C, but they mainly exist in the form of cellulose, which cannot be directly used by plants due to their large molecular weight. On the other hand, the application of chemical fertilizers in recent decades has seriously damaged the health of the soil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C05F11/08C12R1/645
CPCC05F11/08C12N1/14C12N1/145C12R2001/645
Inventor 徐阳春暴彦灼万金鑫韦中沈其荣
Owner NANJING AGRICULTURAL UNIVERSITY
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