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A rice straw degrading fungus Trichoderma pseudokoningeni zjc-1 and its fungal agent

A technology of ZJC-1, Trichoderma pseudocorning, applied in fungi, organic fertilizers, microorganisms, etc., can solve the problems of secondary pollution and high treatment costs, and achieve the effect of protecting the environment, broad application prospects and promoting sustainable development.

Active Publication Date: 2016-06-08
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Straw resource treatment includes physical, chemical and biological methods, but the first two methods are costly and easy to cause secondary pollution. Therefore, microbial treatment is mostly used at present.

Method used

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  • A rice straw degrading fungus Trichoderma pseudokoningeni zjc-1 and its fungal agent
  • A rice straw degrading fungus Trichoderma pseudokoningeni zjc-1 and its fungal agent
  • A rice straw degrading fungus Trichoderma pseudokoningeni zjc-1 and its fungal agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1) Separation and purification of efficient straw degrading strains:

[0030] Weigh 10g soil of decayed branches and leaves from Zijin Mountain, add 90mL sterile water into a 500mL Erlenmeyer flask to make a 10% suspension, 25°C, 170r min -1 Shake culture under the conditions for 1d, then take 10mL suspension and dilute the suspension to 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 sample diluent, choose 10 -3 、10 -4 、10 -5 、10 -6 For four dilution gradients, absorb 100 μL of the sample dilution solution and add it to the rice straw powder medium, and use a sterilized applicator stick to gently spread the sample dilution solution on the surface of the medium evenly, repeat three times for each concentration, and make a mark. The coated plate was placed at 25°C and cultured upside down for 3 days, then taken out. Continue to isolate and purify the single colony grown on the cultured plate through the rice straw powder plate until the pure strain is obtained, spread...

Embodiment 2

[0037] Inoculate the strain ZJC-1 into an Erlenmeyer flask containing 50 mL of rice straw powder medium, culture it statically at 25°C for 7 days, add 30 mL of sterile water to the Erlenmeyer flask, shake it on a shaker for 30 minutes, and filter it with gauze Get the spore suspension bacterial agent after the thing, use physiological saline to adjust the spore concentration in the spore suspension to be 10 6 cfu / mL to obtain bacterial agent.

[0038] The rice straw powder medium is: KH 2 P 4 3.0g, NaNO 3 3.0g, CaCl 2 0.5g, MgSO 4 ·7H 2 O0.5g, FeSO 4 ·7H 2 O7.5mg, MnSO 4 ·H 2 O2.5mg, ZnSO 4 2.0 mg, CoCl 2 3.0mg, rice straw powder 10g, agar 20g, deionized water 1L, pH natural, autoclaved at 121°C for 20min. The rice straw powder refers to the rice straw powder that passes through a 40-mesh sieve after crushing and has a moisture content of less than 1%.

Embodiment 3

[0040] Inoculate the bacterial agent prepared in Example 2 into the liquid medium according to 1% of the volume of the liquid medium, cultivate it for 7 days at 25°C and 170r / min, take an appropriate amount of fermentation liquid every day, and centrifugally filter to remove bacteria and impurities. A crude enzyme extract is obtained. The activity of filter paper enzyme (total cellulase), endocellulase and xylanase in the crude enzyme extract was determined every day.

[0041] Preparation of liquid medium: rice straw powder 20g, (NH 4 ) 2 SO 4 1.4g, KH 2 PO 4 2g, MgSO 4 ·7H 2 O0.3g, CaCl 2 0.4g, tryptone 1g, FeSO 4 ·7H 2 O7.5mg, MnSO 4 ·H 2 O2.5mg, ZnSO 4 2.0 mg, CoCl 2 3.0mg, 1L deionized water, natural pH, autoclave at 121°C for 20min. The rice straw powder is crushed and passed through a 40-mesh sieve with a moisture content of less than 1%.

[0042] Definition and determination of enzyme activity: The determination of enzyme activity refers to the internatio...

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Abstract

The invention belongs to a waste resource utilization technology, and discloses a rice straw degrading fungi trichoderma koningiopsis ZJC-1 and a fungicide thereof. The strain ZJC-1 belongs to Trichoderma koningiopsis, and is collected in China General Microbiological Culture Collection Center (CGMCC) in March, 24th, 2014, and with the collection number of CGMCC NO.8948; the strain ZJC-1 can produce hydrolysis circles on a CMC-congo red culture medium, and can grow on a unique carbon source culture medium of agricultural waste rice straws. The invention further discloses a fungicide comprising the rice straw degrading fungi trichoderma koningiopsis ZJC-1, and a preparation method thereof. The rice straws are degraded by utilizing a microbial fermentation method so as to be converted into an organic fertilizer, thus being capable of changing wastes into valuables, protecting the environment, and having wide application prospects.

Description

technical field [0001] The invention belongs to the intensive agricultural production technology, and relates to a rice straw degrading fungus and its fungal agent, which are specially used for degrading waste rice straws to produce organic fertilizers and realize organic waste resource utilization. Background technique [0002] my country is a large agricultural country. How to deal with crop straws is a problem faced by most agricultural areas. If a large amount of waste straws are not disposed of in time, it will not only affect normal farming, but also become a place for breeding of diseases and insect pests; if it is burned, it will not only seriously It pollutes the environment and also wastes the useful resources and energy contained in the straw. Agricultural waste straw is rich in organic carbon, a large amount of nitrogen, phosphorus, potassium, silicon and other mineral nutrients, as well as a large number of trace elements, and is a very valuable agricultural reso...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C05F11/08C12P19/14C12P19/00C12R1/885
Inventor 徐阳春徐春淼韦中沈其荣
Owner NANJING AGRICULTURAL UNIVERSITY
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